Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS20450

Experiment: 36hr PHYTO2 MOI 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS20445 and BPHYT_RS20450 are separated by 51 nucleotidesBPHYT_RS20450 and BPHYT_RS20455 are separated by 108 nucleotides BPHYT_RS20445: BPHYT_RS20445 - NAD-dependent dehydratase, at 149,568 to 150,632 _RS20445 BPHYT_RS20450: BPHYT_RS20450 - 6-chlorohydroxyquinol-1,2-dioxygenase, at 150,684 to 151,547 _RS20450 BPHYT_RS20455: BPHYT_RS20455 - hypothetical protein, at 151,656 to 152,333 _RS20455 Position (kb) 150 151 152Strain fitness (log2 ratio) -2 -1 0 1at 150.067 kb on + strand, within BPHYT_RS20445at 150.068 kb on - strand, within BPHYT_RS20445at 150.196 kb on + strand, within BPHYT_RS20445at 150.196 kb on + strand, within BPHYT_RS20445at 150.197 kb on - strand, within BPHYT_RS20445at 150.199 kb on + strand, within BPHYT_RS20445at 150.199 kb on + strand, within BPHYT_RS20445at 150.290 kb on - strand, within BPHYT_RS20445at 150.290 kb on - strand, within BPHYT_RS20445at 150.638 kb on + strandat 150.638 kb on + strandat 150.874 kb on + strand, within BPHYT_RS20450at 150.944 kb on + strand, within BPHYT_RS20450at 151.078 kb on + strand, within BPHYT_RS20450at 151.079 kb on - strand, within BPHYT_RS20450at 151.079 kb on - strand, within BPHYT_RS20450at 151.664 kb on + strandat 151.878 kb on - strand, within BPHYT_RS20455at 152.018 kb on - strand, within BPHYT_RS20455at 152.018 kb on - strand, within BPHYT_RS20455at 152.058 kb on - strand, within BPHYT_RS20455at 152.142 kb on - strand, within BPHYT_RS20455at 152.153 kb on + strand, within BPHYT_RS20455at 152.167 kb on + strand, within BPHYT_RS20455at 152.423 kb on + strandat 152.475 kb on + strandat 152.517 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 36hr PHYTO2 MOI 1
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150,067 + BPHYT_RS20445 0.47 +0.4
150,068 - BPHYT_RS20445 0.47 +0.2
150,196 + BPHYT_RS20445 0.59 -1.9
150,196 + BPHYT_RS20445 0.59 -0.4
150,197 - BPHYT_RS20445 0.59 +0.3
150,199 + BPHYT_RS20445 0.59 -0.4
150,199 + BPHYT_RS20445 0.59 -1.0
150,290 - BPHYT_RS20445 0.68 -0.6
150,290 - BPHYT_RS20445 0.68 -0.4
150,638 + +0.6
150,638 + -0.0
150,874 + BPHYT_RS20450 0.22 +0.2
150,944 + BPHYT_RS20450 0.30 -0.5
151,078 + BPHYT_RS20450 0.46 +0.9
151,079 - BPHYT_RS20450 0.46 +0.2
151,079 - BPHYT_RS20450 0.46 -1.7
151,664 + +0.4
151,878 - BPHYT_RS20455 0.33 -1.9
152,018 - BPHYT_RS20455 0.53 -0.2
152,018 - BPHYT_RS20455 0.53 +0.2
152,058 - BPHYT_RS20455 0.59 +0.6
152,142 - BPHYT_RS20455 0.72 +0.9
152,153 + BPHYT_RS20455 0.73 -0.1
152,167 + BPHYT_RS20455 0.75 +0.3
152,423 + -1.6
152,475 + -0.1
152,517 - -0.7

Or see this region's nucleotide sequence