Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS20420

Experiment: 36hr PHYTO2 MOI 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS20415 and BPHYT_RS20420 are separated by 147 nucleotidesBPHYT_RS20420 and BPHYT_RS20425 are separated by 433 nucleotidesBPHYT_RS20425 and BPHYT_RS20430 are separated by 252 nucleotides BPHYT_RS20415: BPHYT_RS20415 - 5-dehydro-4-deoxyglucarate dehydratase, at 143,071 to 143,988 _RS20415 BPHYT_RS20420: BPHYT_RS20420 - DSBA oxidoreductase, at 144,136 to 145,644 _RS20420 BPHYT_RS20425: BPHYT_RS20425 - hypothetical protein, at 146,078 to 146,386 _RS20425 BPHYT_RS20430: BPHYT_RS20430 - 3-dehydroquinate dehydratase, at 146,639 to 147,094 _RS20430 Position (kb) 144 145 146Strain fitness (log2 ratio) -2 -1 0 1 2 3 4at 143.170 kb on + strand, within BPHYT_RS20415at 143.170 kb on + strand, within BPHYT_RS20415at 143.171 kb on - strand, within BPHYT_RS20415at 143.497 kb on + strand, within BPHYT_RS20415at 143.497 kb on + strand, within BPHYT_RS20415at 143.498 kb on - strand, within BPHYT_RS20415at 143.671 kb on + strand, within BPHYT_RS20415at 143.685 kb on + strand, within BPHYT_RS20415at 143.706 kb on - strand, within BPHYT_RS20415at 143.707 kb on + strand, within BPHYT_RS20415at 143.708 kb on - strand, within BPHYT_RS20415at 143.851 kb on + strand, within BPHYT_RS20415at 144.223 kb on - strandat 144.223 kb on - strandat 144.226 kb on + strandat 144.227 kb on - strandat 144.227 kb on - strandat 144.721 kb on - strand, within BPHYT_RS20420at 145.142 kb on + strand, within BPHYT_RS20420at 145.250 kb on + strand, within BPHYT_RS20420at 145.470 kb on - strand, within BPHYT_RS20420at 145.520 kb on + strandat 145.521 kb on - strandat 145.541 kb on + strandat 145.541 kb on + strandat 145.541 kb on + strandat 145.542 kb on - strandat 145.544 kb on + strandat 145.798 kb on + strandat 145.876 kb on + strandat 145.899 kb on + strandat 145.904 kb on - strandat 146.124 kb on + strand, within BPHYT_RS20425at 146.304 kb on - strand, within BPHYT_RS20425at 146.307 kb on - strand, within BPHYT_RS20425at 146.466 kb on + strandat 146.466 kb on + strandat 146.467 kb on - strandat 146.467 kb on - strandat 146.509 kb on - strandat 146.596 kb on + strandat 146.596 kb on + strandat 146.597 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 36hr PHYTO2 MOI 1
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143,170 + BPHYT_RS20415 0.11 +4.0
143,170 + BPHYT_RS20415 0.11 -0.1
143,171 - BPHYT_RS20415 0.11 -0.9
143,497 + BPHYT_RS20415 0.46 -2.0
143,497 + BPHYT_RS20415 0.46 -1.5
143,498 - BPHYT_RS20415 0.47 +0.9
143,671 + BPHYT_RS20415 0.65 -0.7
143,685 + BPHYT_RS20415 0.67 -0.1
143,706 - BPHYT_RS20415 0.69 -1.4
143,707 + BPHYT_RS20415 0.69 -1.2
143,708 - BPHYT_RS20415 0.69 +2.0
143,851 + BPHYT_RS20415 0.85 +2.4
144,223 - -0.7
144,223 - -0.6
144,226 + +0.1
144,227 - -0.5
144,227 - +2.0
144,721 - BPHYT_RS20420 0.39 -1.0
145,142 + BPHYT_RS20420 0.67 +0.7
145,250 + BPHYT_RS20420 0.74 +0.7
145,470 - BPHYT_RS20420 0.88 -1.6
145,520 + -0.5
145,521 - -0.1
145,541 + +0.1
145,541 + -0.7
145,541 + +0.7
145,542 - -0.7
145,544 + +0.6
145,798 + +0.5
145,876 + -0.2
145,899 + +1.6
145,904 - -0.9
146,124 + BPHYT_RS20425 0.15 -0.1
146,304 - BPHYT_RS20425 0.73 -1.3
146,307 - BPHYT_RS20425 0.74 +2.1
146,466 + -2.4
146,466 + -0.4
146,467 - +0.1
146,467 - -1.1
146,509 - +1.2
146,596 + +0.1
146,596 + -0.4
146,597 - -0.5

Or see this region's nucleotide sequence