Experiment: 36hr PHYTO2 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS20215 and BPHYT_RS20220 are separated by 128 nucleotides BPHYT_RS20220 and BPHYT_RS20225 are separated by 87 nucleotides BPHYT_RS20225 and BPHYT_RS20230 overlap by 4 nucleotides BPHYT_RS20230 and BPHYT_RS20235 overlap by 4 nucleotides
BPHYT_RS20215: BPHYT_RS20215 - RNA polymerase sigma 70, at 80,806 to 81,477
_RS20215
BPHYT_RS20220: BPHYT_RS20220 - antibiotic synthesis protein MbtH, at 81,606 to 81,845
_RS20220
BPHYT_RS20225: BPHYT_RS20225 - taurine catabolism dioxygenase tauD/tfdA, at 81,933 to 82,967
_RS20225
BPHYT_RS20230: BPHYT_RS20230 - ABC transporter, at 82,964 to 83,830
_RS20230
BPHYT_RS20235: BPHYT_RS20235 - iron ABC transporter, at 83,827 to 85,935
_RS20235
Position (kb) 81
82
83 Strain fitness (log2 ratio) -2
-1
0
1
2 at 81.094 kb on + strand, within BPHYT_RS20215 at 81.094 kb on + strand, within BPHYT_RS20215 at 81.095 kb on - strand, within BPHYT_RS20215 at 81.507 kb on + strand at 81.664 kb on - strand, within BPHYT_RS20220 at 81.693 kb on + strand, within BPHYT_RS20220 at 81.933 kb on - strand at 82.029 kb on + strand at 82.097 kb on - strand, within BPHYT_RS20225 at 82.176 kb on + strand, within BPHYT_RS20225 at 82.221 kb on + strand, within BPHYT_RS20225 at 82.222 kb on - strand, within BPHYT_RS20225 at 82.222 kb on - strand, within BPHYT_RS20225 at 82.279 kb on - strand, within BPHYT_RS20225 at 82.302 kb on - strand, within BPHYT_RS20225 at 82.645 kb on + strand, within BPHYT_RS20225 at 82.729 kb on - strand, within BPHYT_RS20225 at 82.729 kb on - strand, within BPHYT_RS20225 at 82.914 kb on + strand at 82.915 kb on - strand at 83.809 kb on - strand at 83.884 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO2 MOI 1 remove 81,094 + BPHYT_RS20215 0.43 +0.0 81,094 + BPHYT_RS20215 0.43 -1.6 81,095 - BPHYT_RS20215 0.43 -1.9 81,507 + -0.6 81,664 - BPHYT_RS20220 0.24 -0.8 81,693 + BPHYT_RS20220 0.36 +2.4 81,933 - +0.4 82,029 + -0.5 82,097 - BPHYT_RS20225 0.16 -1.4 82,176 + BPHYT_RS20225 0.23 -0.7 82,221 + BPHYT_RS20225 0.28 -2.0 82,222 - BPHYT_RS20225 0.28 -0.9 82,222 - BPHYT_RS20225 0.28 -1.0 82,279 - BPHYT_RS20225 0.33 -2.0 82,302 - BPHYT_RS20225 0.36 +0.4 82,645 + BPHYT_RS20225 0.69 -0.5 82,729 - BPHYT_RS20225 0.77 -1.7 82,729 - BPHYT_RS20225 0.77 -2.1 82,914 + -1.6 82,915 - -0.1 83,809 - -0.1 83,884 + -0.5
Or see this region's nucleotide sequence