Experiment: 36hr PHYTO2 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS20045 and BPHYT_RS20050 are separated by 117 nucleotides BPHYT_RS20050 and BPHYT_RS20055 are separated by 342 nucleotides BPHYT_RS20055 and BPHYT_RS20060 are separated by 227 nucleotides BPHYT_RS20060 and BPHYT_RS20065 overlap by 1 nucleotides
BPHYT_RS20045: BPHYT_RS20045 - farnesyl-diphosphate farnesyltransferase, at 42,130 to 43,176
_RS20045
BPHYT_RS20050: BPHYT_RS20050 - hypothetical protein, at 43,294 to 43,542
_RS20050
BPHYT_RS20055: BPHYT_RS20055 - hypothetical protein, at 43,885 to 44,202
_RS20055
BPHYT_RS20060: BPHYT_RS20060 - hypothetical protein, at 44,430 to 45,122
_RS20060
BPHYT_RS20065: BPHYT_RS20065 - hypothetical protein, at 45,122 to 45,604
_RS20065
Position (kb) 43
44
45 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 43.219 kb on + strand at 43.242 kb on - strand at 43.312 kb on - strand at 43.776 kb on + strand at 43.843 kb on - strand at 43.886 kb on + strand at 44.069 kb on + strand, within BPHYT_RS20055 at 44.069 kb on + strand, within BPHYT_RS20055 at 44.236 kb on + strand at 44.360 kb on - strand at 44.639 kb on - strand, within BPHYT_RS20060 at 45.145 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO2 MOI 1 remove 43,219 + -0.1 43,242 - -0.9 43,312 - -2.5 43,776 + +0.9 43,843 - +1.1 43,886 + -1.5 44,069 + BPHYT_RS20055 0.58 +1.3 44,069 + BPHYT_RS20055 0.58 -3.6 44,236 + +0.4 44,360 - -2.7 44,639 - BPHYT_RS20060 0.30 +1.3 45,145 + -0.6
Or see this region's nucleotide sequence