Experiment: 36hr PHYTO2 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS19345 and BPHYT_RS19350 are separated by 144 nucleotides BPHYT_RS19350 and BPHYT_RS19355 are separated by 88 nucleotides
BPHYT_RS19345: BPHYT_RS19345 - thiamine pyrophosphate protein, at 4,345,796 to 4,347,514
_RS19345
BPHYT_RS19350: BPHYT_RS19350 - amino acid ABC transporter substrate-binding protein, at 4,347,659 to 4,348,807
_RS19350
BPHYT_RS19355: BPHYT_RS19355 - bifunctional proline dehydrogenase/pyrroline-5-carboxylate dehydrogenase, at 4,348,896 to 4,352,825
_RS19355
Position (kb) 4347
4348
4349 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4 at 4346.690 kb on + strand at 4346.888 kb on + strand, within BPHYT_RS19345 at 4346.888 kb on + strand, within BPHYT_RS19345 at 4347.005 kb on + strand, within BPHYT_RS19345 at 4347.035 kb on + strand, within BPHYT_RS19345 at 4347.084 kb on - strand, within BPHYT_RS19345 at 4347.084 kb on - strand, within BPHYT_RS19345 at 4347.120 kb on - strand, within BPHYT_RS19345 at 4347.201 kb on - strand, within BPHYT_RS19345 at 4347.288 kb on + strand, within BPHYT_RS19345 at 4347.288 kb on + strand, within BPHYT_RS19345 at 4347.289 kb on - strand, within BPHYT_RS19345 at 4347.289 kb on - strand, within BPHYT_RS19345 at 4347.657 kb on - strand at 4347.660 kb on + strand at 4347.661 kb on - strand at 4347.694 kb on + strand at 4347.695 kb on - strand at 4347.695 kb on - strand at 4347.712 kb on - strand at 4347.712 kb on - strand at 4347.721 kb on + strand at 4347.722 kb on - strand at 4347.811 kb on - strand, within BPHYT_RS19350 at 4347.873 kb on + strand, within BPHYT_RS19350 at 4347.889 kb on - strand, within BPHYT_RS19350 at 4347.921 kb on + strand, within BPHYT_RS19350 at 4347.933 kb on + strand, within BPHYT_RS19350 at 4347.934 kb on - strand, within BPHYT_RS19350 at 4348.122 kb on + strand, within BPHYT_RS19350 at 4348.122 kb on + strand, within BPHYT_RS19350 at 4348.125 kb on + strand, within BPHYT_RS19350 at 4348.125 kb on + strand, within BPHYT_RS19350 at 4348.126 kb on - strand, within BPHYT_RS19350 at 4348.198 kb on - strand, within BPHYT_RS19350 at 4348.402 kb on - strand, within BPHYT_RS19350 at 4348.449 kb on + strand, within BPHYT_RS19350 at 4348.449 kb on + strand, within BPHYT_RS19350 at 4348.645 kb on + strand, within BPHYT_RS19350 at 4348.671 kb on + strand, within BPHYT_RS19350 at 4348.807 kb on + strand at 4348.807 kb on + strand at 4348.808 kb on - strand at 4348.808 kb on - strand at 4348.808 kb on - strand at 4348.808 kb on - strand at 4348.808 kb on - strand at 4348.822 kb on - strand at 4348.984 kb on + strand at 4349.277 kb on - strand at 4349.308 kb on + strand, within BPHYT_RS19355 at 4349.308 kb on + strand, within BPHYT_RS19355 at 4349.308 kb on + strand, within BPHYT_RS19355 at 4349.374 kb on + strand, within BPHYT_RS19355 at 4349.375 kb on - strand, within BPHYT_RS19355 at 4349.554 kb on + strand, within BPHYT_RS19355 at 4349.555 kb on - strand, within BPHYT_RS19355 at 4349.555 kb on - strand, within BPHYT_RS19355 at 4349.650 kb on + strand, within BPHYT_RS19355 at 4349.650 kb on + strand, within BPHYT_RS19355 at 4349.651 kb on - strand, within BPHYT_RS19355 at 4349.651 kb on - strand, within BPHYT_RS19355 at 4349.651 kb on - strand, within BPHYT_RS19355 at 4349.709 kb on - strand, within BPHYT_RS19355
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO2 MOI 1 remove 4,346,690 + +4.4 4,346,888 + BPHYT_RS19345 0.64 +0.7 4,346,888 + BPHYT_RS19345 0.64 +0.4 4,347,005 + BPHYT_RS19345 0.70 -0.4 4,347,035 + BPHYT_RS19345 0.72 -0.8 4,347,084 - BPHYT_RS19345 0.75 -0.6 4,347,084 - BPHYT_RS19345 0.75 +0.4 4,347,120 - BPHYT_RS19345 0.77 -0.3 4,347,201 - BPHYT_RS19345 0.82 -0.9 4,347,288 + BPHYT_RS19345 0.87 +0.9 4,347,288 + BPHYT_RS19345 0.87 +0.1 4,347,289 - BPHYT_RS19345 0.87 +0.5 4,347,289 - BPHYT_RS19345 0.87 -1.1 4,347,657 - -0.1 4,347,660 + +0.4 4,347,661 - +0.3 4,347,694 + -1.5 4,347,695 - -0.7 4,347,695 - +0.8 4,347,712 - +0.3 4,347,712 - +0.3 4,347,721 + -0.6 4,347,722 - +0.0 4,347,811 - BPHYT_RS19350 0.13 -1.1 4,347,873 + BPHYT_RS19350 0.19 +0.8 4,347,889 - BPHYT_RS19350 0.20 +0.5 4,347,921 + BPHYT_RS19350 0.23 -0.8 4,347,933 + BPHYT_RS19350 0.24 -1.5 4,347,934 - BPHYT_RS19350 0.24 -1.1 4,348,122 + BPHYT_RS19350 0.40 -1.0 4,348,122 + BPHYT_RS19350 0.40 -2.6 4,348,125 + BPHYT_RS19350 0.41 +3.6 4,348,125 + BPHYT_RS19350 0.41 -1.3 4,348,126 - BPHYT_RS19350 0.41 -0.4 4,348,198 - BPHYT_RS19350 0.47 -0.5 4,348,402 - BPHYT_RS19350 0.65 -0.1 4,348,449 + BPHYT_RS19350 0.69 +0.8 4,348,449 + BPHYT_RS19350 0.69 -0.9 4,348,645 + BPHYT_RS19350 0.86 -0.8 4,348,671 + BPHYT_RS19350 0.88 -0.9 4,348,807 + +1.4 4,348,807 + -0.1 4,348,808 - -0.7 4,348,808 - +0.8 4,348,808 - +0.3 4,348,808 - -0.0 4,348,808 - +0.5 4,348,822 - -0.0 4,348,984 + -1.2 4,349,277 - +0.9 4,349,308 + BPHYT_RS19355 0.10 +0.5 4,349,308 + BPHYT_RS19355 0.10 -0.3 4,349,308 + BPHYT_RS19355 0.10 -2.2 4,349,374 + BPHYT_RS19355 0.12 +0.5 4,349,375 - BPHYT_RS19355 0.12 +2.4 4,349,554 + BPHYT_RS19355 0.17 -0.2 4,349,555 - BPHYT_RS19355 0.17 -0.6 4,349,555 - BPHYT_RS19355 0.17 +2.1 4,349,650 + BPHYT_RS19355 0.19 +0.3 4,349,650 + BPHYT_RS19355 0.19 +0.1 4,349,651 - BPHYT_RS19355 0.19 +1.4 4,349,651 - BPHYT_RS19355 0.19 +0.8 4,349,651 - BPHYT_RS19355 0.19 +0.9 4,349,709 - BPHYT_RS19355 0.21 +1.0
Or see this region's nucleotide sequence