Experiment: 36hr PHYTO2 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS16315 and BPHYT_RS16320 are separated by 244 nucleotides BPHYT_RS16320 and BPHYT_RS16325 are separated by 98 nucleotides
BPHYT_RS16315: BPHYT_RS16315 - peptidase M48 Ste24p, at 3,701,945 to 3,703,654
_RS16315
BPHYT_RS16320: BPHYT_RS16320 - cyclic pyranopterin monophosphate synthase accessory protein, at 3,703,899 to 3,704,375
_RS16320
BPHYT_RS16325: BPHYT_RS16325 - polymerase, at 3,704,474 to 3,706,258
_RS16325
Position (kb)
3703
3704
3705 Strain fitness (log2 ratio)
-2
-1
0
1 at 3702.969 kb on - strand, within BPHYT_RS16315 at 3702.988 kb on - strand, within BPHYT_RS16315 at 3702.988 kb on - strand, within BPHYT_RS16315 at 3703.203 kb on + strand, within BPHYT_RS16315 at 3703.204 kb on - strand, within BPHYT_RS16315 at 3703.204 kb on - strand, within BPHYT_RS16315 at 3703.204 kb on - strand, within BPHYT_RS16315 at 3703.251 kb on + strand, within BPHYT_RS16315 at 3703.252 kb on - strand, within BPHYT_RS16315 at 3703.278 kb on + strand, within BPHYT_RS16315 at 3703.342 kb on + strand, within BPHYT_RS16315 at 3703.423 kb on + strand, within BPHYT_RS16315 at 3703.455 kb on + strand, within BPHYT_RS16315 at 3703.456 kb on - strand, within BPHYT_RS16315 at 3703.564 kb on + strand at 3703.703 kb on - strand at 3703.703 kb on - strand at 3703.727 kb on + strand at 3703.727 kb on + strand at 3703.868 kb on + strand at 3703.868 kb on + strand at 3703.868 kb on + strand at 3703.868 kb on + strand at 3703.869 kb on - strand at 3703.873 kb on + strand at 3703.874 kb on - strand at 3703.885 kb on + strand at 3703.940 kb on + strand at 3703.940 kb on + strand at 3703.940 kb on + strand at 3704.277 kb on + strand, within BPHYT_RS16320 at 3704.278 kb on - strand, within BPHYT_RS16320 at 3704.278 kb on - strand, within BPHYT_RS16320 at 3704.278 kb on - strand, within BPHYT_RS16320 at 3704.408 kb on + strand at 3704.467 kb on + strand at 3704.468 kb on - strand at 3704.593 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO2 MOI 1 remove 3,702,969 - BPHYT_RS16315 0.60 +0.8 3,702,988 - BPHYT_RS16315 0.61 +0.5 3,702,988 - BPHYT_RS16315 0.61 +0.8 3,703,203 + BPHYT_RS16315 0.74 +1.6 3,703,204 - BPHYT_RS16315 0.74 -0.3 3,703,204 - BPHYT_RS16315 0.74 -0.4 3,703,204 - BPHYT_RS16315 0.74 -0.4 3,703,251 + BPHYT_RS16315 0.76 -0.4 3,703,252 - BPHYT_RS16315 0.76 +0.1 3,703,278 + BPHYT_RS16315 0.78 -1.8 3,703,342 + BPHYT_RS16315 0.82 -1.5 3,703,423 + BPHYT_RS16315 0.86 +0.3 3,703,455 + BPHYT_RS16315 0.88 +0.4 3,703,456 - BPHYT_RS16315 0.88 -0.8 3,703,564 + -0.4 3,703,703 - +1.1 3,703,703 - +0.3 3,703,727 + +0.2 3,703,727 + -1.1 3,703,868 + -2.0 3,703,868 + +0.3 3,703,868 + +1.0 3,703,868 + -0.3 3,703,869 - +0.9 3,703,873 + +1.6 3,703,874 - -0.5 3,703,885 + +1.3 3,703,940 + +0.3 3,703,940 + +0.1 3,703,940 + -0.8 3,704,277 + BPHYT_RS16320 0.79 -2.3 3,704,278 - BPHYT_RS16320 0.79 -0.9 3,704,278 - BPHYT_RS16320 0.79 -1.2 3,704,278 - BPHYT_RS16320 0.79 -0.8 3,704,408 + +0.4 3,704,467 + -0.1 3,704,468 - -0.6 3,704,593 - -1.3
Or see this region's nucleotide sequence