Experiment: 36hr PHYTO2 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS15520 and BPHYT_RS15525 overlap by 4 nucleotides BPHYT_RS15525 and BPHYT_RS15530 are separated by 7 nucleotides BPHYT_RS15530 and BPHYT_RS15535 are separated by 123 nucleotides
BPHYT_RS15520: BPHYT_RS15520 - D-aminopeptidase, at 3,521,206 to 3,522,306
_RS15520
BPHYT_RS15525: BPHYT_RS15525 - glutathione ABC transporter permease GsiD, at 3,522,303 to 3,523,208
_RS15525
BPHYT_RS15530: BPHYT_RS15530 - glutathione ABC transporter permease, at 3,523,216 to 3,524,136
_RS15530
BPHYT_RS15535: BPHYT_RS15535 - glutathione ABC transporter substrate-binding protein GsiB, at 3,524,260 to 3,525,822
_RS15535
Position (kb)
3523
3524
3525 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 3522.545 kb on - strand, within BPHYT_RS15525 at 3522.633 kb on - strand, within BPHYT_RS15525 at 3522.634 kb on + strand, within BPHYT_RS15525 at 3522.634 kb on + strand, within BPHYT_RS15525 at 3522.634 kb on + strand, within BPHYT_RS15525 at 3522.634 kb on + strand, within BPHYT_RS15525 at 3522.634 kb on + strand, within BPHYT_RS15525 at 3522.635 kb on - strand, within BPHYT_RS15525 at 3522.832 kb on + strand, within BPHYT_RS15525 at 3522.835 kb on + strand, within BPHYT_RS15525 at 3522.836 kb on - strand, within BPHYT_RS15525 at 3523.250 kb on + strand at 3523.250 kb on + strand at 3523.619 kb on + strand, within BPHYT_RS15530 at 3523.619 kb on + strand, within BPHYT_RS15530 at 3523.619 kb on + strand, within BPHYT_RS15530 at 3523.619 kb on + strand, within BPHYT_RS15530 at 3523.620 kb on - strand, within BPHYT_RS15530 at 3524.052 kb on + strand at 3524.052 kb on + strand at 3524.053 kb on - strand at 3524.053 kb on - strand at 3524.053 kb on - strand at 3524.187 kb on + strand at 3524.310 kb on - strand at 3524.483 kb on + strand, within BPHYT_RS15535 at 3524.582 kb on + strand, within BPHYT_RS15535 at 3524.586 kb on - strand, within BPHYT_RS15535 at 3524.586 kb on - strand, within BPHYT_RS15535 at 3524.733 kb on - strand, within BPHYT_RS15535 at 3524.738 kb on + strand, within BPHYT_RS15535 at 3524.931 kb on - strand, within BPHYT_RS15535 at 3524.992 kb on - strand, within BPHYT_RS15535 at 3524.993 kb on + strand, within BPHYT_RS15535 at 3524.994 kb on - strand, within BPHYT_RS15535
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO2 MOI 1 remove 3,522,545 - BPHYT_RS15525 0.27 +0.7 3,522,633 - BPHYT_RS15525 0.36 -0.5 3,522,634 + BPHYT_RS15525 0.37 +0.1 3,522,634 + BPHYT_RS15525 0.37 -0.2 3,522,634 + BPHYT_RS15525 0.37 -0.5 3,522,634 + BPHYT_RS15525 0.37 -1.3 3,522,634 + BPHYT_RS15525 0.37 +0.6 3,522,635 - BPHYT_RS15525 0.37 +0.2 3,522,832 + BPHYT_RS15525 0.58 +0.6 3,522,835 + BPHYT_RS15525 0.59 +1.6 3,522,836 - BPHYT_RS15525 0.59 +0.6 3,523,250 + +0.1 3,523,250 + +1.0 3,523,619 + BPHYT_RS15530 0.44 -0.7 3,523,619 + BPHYT_RS15530 0.44 -2.6 3,523,619 + BPHYT_RS15530 0.44 -1.7 3,523,619 + BPHYT_RS15530 0.44 -0.7 3,523,620 - BPHYT_RS15530 0.44 +0.0 3,524,052 + -0.6 3,524,052 + +0.2 3,524,053 - -1.2 3,524,053 - -0.5 3,524,053 - -0.3 3,524,187 + -1.3 3,524,310 - +0.2 3,524,483 + BPHYT_RS15535 0.14 +3.3 3,524,582 + BPHYT_RS15535 0.21 +0.6 3,524,586 - BPHYT_RS15535 0.21 -1.2 3,524,586 - BPHYT_RS15535 0.21 -0.3 3,524,733 - BPHYT_RS15535 0.30 +0.0 3,524,738 + BPHYT_RS15535 0.31 -1.3 3,524,931 - BPHYT_RS15535 0.43 +0.7 3,524,992 - BPHYT_RS15535 0.47 +0.8 3,524,993 + BPHYT_RS15535 0.47 -1.8 3,524,994 - BPHYT_RS15535 0.47 -2.3
Or see this region's nucleotide sequence