Experiment: 36hr PHYTO2 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS14120 and BPHYT_RS14125 are separated by 26 nucleotides BPHYT_RS14125 and BPHYT_RS14130 are separated by 28 nucleotides BPHYT_RS14130 and BPHYT_RS14135 are separated by 17 nucleotides BPHYT_RS14135 and BPHYT_RS14140 overlap by 4 nucleotides
BPHYT_RS14120: BPHYT_RS14120 - chemotaxis protein CheY, at 3,225,241 to 3,225,942
_RS14120
BPHYT_RS14125: BPHYT_RS14125 - transcriptional regulator, at 3,225,969 to 3,226,673
_RS14125
BPHYT_RS14130: BPHYT_RS14130 - phosphate ABC transporter ATP-binding protein, at 3,226,702 to 3,227,550
_RS14130
BPHYT_RS14135: BPHYT_RS14135 - phosphate transporter permease subunit PtsA, at 3,227,568 to 3,228,464
_RS14135
BPHYT_RS14140: BPHYT_RS14140 - phosphate transporter permease subunit PstC, at 3,228,461 to 3,229,462
_RS14140
Position (kb)
3226
3227
3228 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4
5 at 3225.714 kb on + strand, within BPHYT_RS14120 at 3225.845 kb on + strand, within BPHYT_RS14120 at 3225.846 kb on - strand, within BPHYT_RS14120 at 3225.971 kb on - strand at 3226.036 kb on + strand at 3226.147 kb on + strand, within BPHYT_RS14125 at 3226.148 kb on - strand, within BPHYT_RS14125 at 3226.679 kb on - strand at 3226.679 kb on - strand at 3226.688 kb on + strand at 3226.688 kb on + strand at 3226.688 kb on + strand at 3226.688 kb on + strand at 3226.689 kb on - strand at 3226.724 kb on + strand at 3226.724 kb on + strand at 3226.799 kb on + strand, within BPHYT_RS14130 at 3226.799 kb on + strand, within BPHYT_RS14130 at 3226.803 kb on + strand, within BPHYT_RS14130 at 3226.803 kb on + strand, within BPHYT_RS14130 at 3226.803 kb on + strand, within BPHYT_RS14130 at 3226.803 kb on + strand, within BPHYT_RS14130 at 3226.803 kb on + strand, within BPHYT_RS14130 at 3226.805 kb on + strand, within BPHYT_RS14130 at 3226.805 kb on + strand, within BPHYT_RS14130 at 3226.814 kb on + strand, within BPHYT_RS14130 at 3226.868 kb on + strand, within BPHYT_RS14130 at 3227.297 kb on + strand, within BPHYT_RS14130 at 3227.297 kb on + strand, within BPHYT_RS14130 at 3227.297 kb on + strand, within BPHYT_RS14130 at 3227.297 kb on + strand, within BPHYT_RS14130 at 3227.408 kb on + strand, within BPHYT_RS14130 at 3227.544 kb on + strand at 3227.569 kb on + strand at 3227.570 kb on - strand at 3227.570 kb on - strand at 3227.689 kb on + strand, within BPHYT_RS14135 at 3227.689 kb on + strand, within BPHYT_RS14135 at 3228.300 kb on - strand, within BPHYT_RS14135 at 3228.444 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO2 MOI 1 remove 3,225,714 + BPHYT_RS14120 0.67 -1.3 3,225,845 + BPHYT_RS14120 0.86 -1.0 3,225,846 - BPHYT_RS14120 0.86 -0.3 3,225,971 - -1.3 3,226,036 + +1.3 3,226,147 + BPHYT_RS14125 0.25 -0.6 3,226,148 - BPHYT_RS14125 0.25 -0.2 3,226,679 - +0.2 3,226,679 - +1.0 3,226,688 + +0.1 3,226,688 + -0.3 3,226,688 + +1.0 3,226,688 + -2.4 3,226,689 - +1.8 3,226,724 + -0.1 3,226,724 + +0.4 3,226,799 + BPHYT_RS14130 0.11 +0.0 3,226,799 + BPHYT_RS14130 0.11 -0.5 3,226,803 + BPHYT_RS14130 0.12 -1.3 3,226,803 + BPHYT_RS14130 0.12 +1.3 3,226,803 + BPHYT_RS14130 0.12 +1.8 3,226,803 + BPHYT_RS14130 0.12 +0.6 3,226,803 + BPHYT_RS14130 0.12 +0.5 3,226,805 + BPHYT_RS14130 0.12 +0.5 3,226,805 + BPHYT_RS14130 0.12 -0.2 3,226,814 + BPHYT_RS14130 0.13 +2.3 3,226,868 + BPHYT_RS14130 0.20 +2.2 3,227,297 + BPHYT_RS14130 0.70 +0.9 3,227,297 + BPHYT_RS14130 0.70 -0.2 3,227,297 + BPHYT_RS14130 0.70 +1.4 3,227,297 + BPHYT_RS14130 0.70 +5.0 3,227,408 + BPHYT_RS14130 0.83 +0.1 3,227,544 + +1.8 3,227,569 + -0.3 3,227,570 - +0.4 3,227,570 - +0.2 3,227,689 + BPHYT_RS14135 0.13 -0.3 3,227,689 + BPHYT_RS14135 0.13 +1.4 3,228,300 - BPHYT_RS14135 0.82 +2.6 3,228,444 - +2.5
Or see this region's nucleotide sequence