Experiment: 36hr PHYTO2 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS13910 and BPHYT_RS13915 overlap by 4 nucleotides BPHYT_RS13915 and BPHYT_RS13920 are separated by 10 nucleotides BPHYT_RS13920 and BPHYT_RS13925 overlap by 4 nucleotides
BPHYT_RS13910: BPHYT_RS13910 - 5-dehydro-2-deoxygluconokinase (EC 2.7.1.92); possible 5-dehydro-2-deoxyphosphogluconate aldolase DUF2090 (EC 4.1.2.29) (from data) , at 3,175,184 to 3,177,229
_RS13910
BPHYT_RS13915: BPHYT_RS13915 - 3D-(3,5/4)-trihydroxycyclohexane-1,2-dione hydrolase, at 3,177,226 to 3,179,199
_RS13915
BPHYT_RS13920: BPHYT_RS13920 - Myo-inosose-2 dehydratase (EC 4.2.1.44) (from data) , at 3,179,210 to 3,180,127
_RS13920
BPHYT_RS13925: BPHYT_RS13925 - 5-deoxy-D-glucuronate isomerase (EC 5.3.1.30) (from data) , at 3,180,124 to 3,180,924
_RS13925
Position (kb)
3177
3178
3179
3180 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3176.654 kb on - strand, within BPHYT_RS13910 at 3176.847 kb on - strand, within BPHYT_RS13910 at 3177.240 kb on - strand at 3177.683 kb on + strand, within BPHYT_RS13915 at 3178.024 kb on + strand, within BPHYT_RS13915 at 3178.723 kb on + strand at 3179.009 kb on - strand at 3179.009 kb on - strand at 3179.230 kb on + strand at 3179.399 kb on + strand, within BPHYT_RS13920 at 3179.399 kb on + strand, within BPHYT_RS13920 at 3179.426 kb on + strand, within BPHYT_RS13920 at 3179.493 kb on + strand, within BPHYT_RS13920 at 3179.525 kb on + strand, within BPHYT_RS13920 at 3179.612 kb on + strand, within BPHYT_RS13920 at 3179.613 kb on - strand, within BPHYT_RS13920 at 3179.644 kb on + strand, within BPHYT_RS13920 at 3179.644 kb on + strand, within BPHYT_RS13920
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO2 MOI 1 remove 3,176,654 - BPHYT_RS13910 0.72 +1.8 3,176,847 - BPHYT_RS13910 0.81 +0.2 3,177,240 - -0.7 3,177,683 + BPHYT_RS13915 0.23 -0.2 3,178,024 + BPHYT_RS13915 0.40 +0.2 3,178,723 + +1.0 3,179,009 - -0.9 3,179,009 - +0.4 3,179,230 + -0.4 3,179,399 + BPHYT_RS13920 0.21 +0.1 3,179,399 + BPHYT_RS13920 0.21 -2.5 3,179,426 + BPHYT_RS13920 0.24 -0.7 3,179,493 + BPHYT_RS13920 0.31 -0.2 3,179,525 + BPHYT_RS13920 0.34 +0.6 3,179,612 + BPHYT_RS13920 0.44 +0.8 3,179,613 - BPHYT_RS13920 0.44 -0.4 3,179,644 + BPHYT_RS13920 0.47 +0.1 3,179,644 + BPHYT_RS13920 0.47 -0.1
Or see this region's nucleotide sequence