Experiment: 36hr PHYTO2 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS12960 and BPHYT_RS12965 are separated by 147 nucleotides BPHYT_RS12965 and BPHYT_RS12970 are separated by 58 nucleotides
BPHYT_RS12960: BPHYT_RS12960 - membrane protein, at 2,958,719 to 2,959,552
_RS12960
BPHYT_RS12965: BPHYT_RS12965 - fatty acid hydroxylase, at 2,959,700 to 2,960,698
_RS12965
BPHYT_RS12970: BPHYT_RS12970 - hypothetical protein, at 2,960,757 to 2,961,776
_RS12970
Position (kb)
2959
2960
2961 Strain fitness (log2 ratio)
-1
0
1
2
3
4
5 at 2958.795 kb on + strand at 2958.795 kb on + strand at 2958.796 kb on - strand at 2958.809 kb on + strand, within BPHYT_RS12960 at 2958.810 kb on - strand, within BPHYT_RS12960 at 2958.821 kb on + strand, within BPHYT_RS12960 at 2958.822 kb on - strand, within BPHYT_RS12960 at 2958.837 kb on + strand, within BPHYT_RS12960 at 2958.837 kb on + strand, within BPHYT_RS12960 at 2958.837 kb on + strand, within BPHYT_RS12960 at 2958.837 kb on + strand, within BPHYT_RS12960 at 2958.838 kb on - strand, within BPHYT_RS12960 at 2958.838 kb on - strand, within BPHYT_RS12960 at 2959.023 kb on + strand, within BPHYT_RS12960 at 2959.039 kb on - strand, within BPHYT_RS12960 at 2959.168 kb on - strand, within BPHYT_RS12960 at 2959.306 kb on + strand, within BPHYT_RS12960 at 2959.363 kb on - strand, within BPHYT_RS12960 at 2959.648 kb on + strand at 2959.648 kb on + strand at 2959.649 kb on - strand at 2959.917 kb on + strand, within BPHYT_RS12965 at 2960.007 kb on + strand, within BPHYT_RS12965 at 2960.008 kb on - strand, within BPHYT_RS12965 at 2960.069 kb on + strand, within BPHYT_RS12965 at 2960.242 kb on - strand, within BPHYT_RS12965 at 2960.274 kb on + strand, within BPHYT_RS12965 at 2960.275 kb on - strand, within BPHYT_RS12965 at 2960.347 kb on + strand, within BPHYT_RS12965 at 2960.347 kb on + strand, within BPHYT_RS12965 at 2960.348 kb on - strand, within BPHYT_RS12965 at 2960.440 kb on + strand, within BPHYT_RS12965 at 2960.440 kb on + strand, within BPHYT_RS12965 at 2960.441 kb on - strand, within BPHYT_RS12965 at 2960.441 kb on - strand, within BPHYT_RS12965 at 2960.441 kb on - strand, within BPHYT_RS12965 at 2960.442 kb on + strand, within BPHYT_RS12965 at 2960.443 kb on - strand, within BPHYT_RS12965 at 2960.443 kb on - strand, within BPHYT_RS12965 at 2960.443 kb on - strand at 2960.443 kb on - strand, within BPHYT_RS12965 at 2960.523 kb on + strand, within BPHYT_RS12965 at 2960.562 kb on + strand, within BPHYT_RS12965 at 2960.563 kb on - strand, within BPHYT_RS12965 at 2960.574 kb on + strand, within BPHYT_RS12965 at 2960.590 kb on - strand, within BPHYT_RS12965 at 2960.637 kb on + strand at 2960.638 kb on - strand at 2960.800 kb on + strand at 2960.801 kb on - strand at 2960.801 kb on - strand at 2960.894 kb on + strand, within BPHYT_RS12970 at 2960.895 kb on - strand, within BPHYT_RS12970 at 2960.905 kb on + strand, within BPHYT_RS12970 at 2960.975 kb on + strand, within BPHYT_RS12970 at 2960.976 kb on - strand, within BPHYT_RS12970 at 2961.131 kb on - strand, within BPHYT_RS12970 at 2961.160 kb on + strand, within BPHYT_RS12970 at 2961.161 kb on - strand, within BPHYT_RS12970 at 2961.302 kb on + strand, within BPHYT_RS12970 at 2961.311 kb on + strand, within BPHYT_RS12970 at 2961.376 kb on + strand, within BPHYT_RS12970 at 2961.377 kb on - strand, within BPHYT_RS12970 at 2961.377 kb on - strand, within BPHYT_RS12970 at 2961.382 kb on + strand, within BPHYT_RS12970 at 2961.382 kb on + strand, within BPHYT_RS12970 at 2961.382 kb on + strand, within BPHYT_RS12970 at 2961.383 kb on - strand, within BPHYT_RS12970 at 2961.383 kb on - strand, within BPHYT_RS12970 at 2961.383 kb on - strand, within BPHYT_RS12970
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO2 MOI 1 remove 2,958,795 + -0.8 2,958,795 + -0.5 2,958,796 - +0.5 2,958,809 + BPHYT_RS12960 0.11 -1.0 2,958,810 - BPHYT_RS12960 0.11 +0.2 2,958,821 + BPHYT_RS12960 0.12 +0.7 2,958,822 - BPHYT_RS12960 0.12 -0.7 2,958,837 + BPHYT_RS12960 0.14 -0.2 2,958,837 + BPHYT_RS12960 0.14 +0.0 2,958,837 + BPHYT_RS12960 0.14 +0.5 2,958,837 + BPHYT_RS12960 0.14 +0.6 2,958,838 - BPHYT_RS12960 0.14 +1.8 2,958,838 - BPHYT_RS12960 0.14 +0.0 2,959,023 + BPHYT_RS12960 0.36 -0.7 2,959,039 - BPHYT_RS12960 0.38 -0.4 2,959,168 - BPHYT_RS12960 0.54 -0.2 2,959,306 + BPHYT_RS12960 0.70 -0.3 2,959,363 - BPHYT_RS12960 0.77 +1.1 2,959,648 + +0.3 2,959,648 + -0.0 2,959,649 - +0.4 2,959,917 + BPHYT_RS12965 0.22 -0.3 2,960,007 + BPHYT_RS12965 0.31 -0.6 2,960,008 - BPHYT_RS12965 0.31 -0.1 2,960,069 + BPHYT_RS12965 0.37 -0.6 2,960,242 - BPHYT_RS12965 0.54 +0.0 2,960,274 + BPHYT_RS12965 0.57 -0.4 2,960,275 - BPHYT_RS12965 0.58 +5.0 2,960,347 + BPHYT_RS12965 0.65 +0.8 2,960,347 + BPHYT_RS12965 0.65 +0.9 2,960,348 - BPHYT_RS12965 0.65 +0.1 2,960,440 + BPHYT_RS12965 0.74 -0.5 2,960,440 + BPHYT_RS12965 0.74 +1.3 2,960,441 - BPHYT_RS12965 0.74 -0.2 2,960,441 - BPHYT_RS12965 0.74 -0.3 2,960,441 - BPHYT_RS12965 0.74 -0.2 2,960,442 + BPHYT_RS12965 0.74 +0.7 2,960,443 - BPHYT_RS12965 0.74 +1.0 2,960,443 - BPHYT_RS12965 0.74 -0.6 2,960,443 - +2.4 2,960,443 - BPHYT_RS12965 0.74 -0.0 2,960,523 + BPHYT_RS12965 0.82 -1.0 2,960,562 + BPHYT_RS12965 0.86 -0.6 2,960,563 - BPHYT_RS12965 0.86 -1.2 2,960,574 + BPHYT_RS12965 0.87 +1.7 2,960,590 - BPHYT_RS12965 0.89 -0.4 2,960,637 + -0.0 2,960,638 - -1.5 2,960,800 + +1.6 2,960,801 - +0.0 2,960,801 - +0.1 2,960,894 + BPHYT_RS12970 0.13 +2.0 2,960,895 - BPHYT_RS12970 0.14 +0.8 2,960,905 + BPHYT_RS12970 0.15 -0.5 2,960,975 + BPHYT_RS12970 0.21 -0.2 2,960,976 - BPHYT_RS12970 0.21 -1.4 2,961,131 - BPHYT_RS12970 0.37 -0.1 2,961,160 + BPHYT_RS12970 0.40 -0.6 2,961,161 - BPHYT_RS12970 0.40 -0.9 2,961,302 + BPHYT_RS12970 0.53 +0.6 2,961,311 + BPHYT_RS12970 0.54 +0.6 2,961,376 + BPHYT_RS12970 0.61 -1.7 2,961,377 - BPHYT_RS12970 0.61 +0.3 2,961,377 - BPHYT_RS12970 0.61 -0.6 2,961,382 + BPHYT_RS12970 0.61 +0.8 2,961,382 + BPHYT_RS12970 0.61 +0.3 2,961,382 + BPHYT_RS12970 0.61 -0.7 2,961,383 - BPHYT_RS12970 0.61 -0.4 2,961,383 - BPHYT_RS12970 0.61 -1.0 2,961,383 - BPHYT_RS12970 0.61 +0.8
Or see this region's nucleotide sequence