Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS12740

Experiment: 36hr PHYTO2 MOI 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS12735 and BPHYT_RS12740 are separated by 149 nucleotidesBPHYT_RS12740 and BPHYT_RS12745 are separated by 313 nucleotides BPHYT_RS12735: BPHYT_RS12735 - peptide chain release factor 2, at 2,904,752 to 2,905,498 _RS12735 BPHYT_RS12740: BPHYT_RS12740 - lysine--tRNA ligase, at 2,905,648 to 2,907,204 _RS12740 BPHYT_RS12745: BPHYT_RS12745 - membrane protein, at 2,907,518 to 2,908,261 _RS12745 Position (kb) 2905 2906 2907 2908Strain fitness (log2 ratio) -2 -1 0 1at 2907.397 kb on + strandat 2907.397 kb on + strandat 2907.398 kb on - strandat 2907.398 kb on - strandat 2907.400 kb on - strandat 2907.400 kb on - strandat 2907.480 kb on + strandat 2907.860 kb on + strand, within BPHYT_RS12745at 2907.860 kb on + strand, within BPHYT_RS12745at 2907.893 kb on - strand, within BPHYT_RS12745at 2907.893 kb on - strand, within BPHYT_RS12745at 2907.896 kb on + strand, within BPHYT_RS12745at 2907.897 kb on - strand, within BPHYT_RS12745at 2907.897 kb on - strand, within BPHYT_RS12745at 2907.897 kb on - strand, within BPHYT_RS12745at 2907.926 kb on + strand, within BPHYT_RS12745at 2907.927 kb on - strand, within BPHYT_RS12745at 2908.190 kb on + strandat 2908.190 kb on + strandat 2908.190 kb on + strandat 2908.191 kb on - strandat 2908.191 kb on - strandat 2908.191 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 36hr PHYTO2 MOI 1
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2,907,397 + -1.0
2,907,397 + -0.3
2,907,398 - +0.1
2,907,398 - +0.7
2,907,400 - -0.1
2,907,400 - -0.7
2,907,480 + -0.0
2,907,860 + BPHYT_RS12745 0.46 -0.5
2,907,860 + BPHYT_RS12745 0.46 -0.7
2,907,893 - BPHYT_RS12745 0.50 -0.2
2,907,893 - BPHYT_RS12745 0.50 +0.6
2,907,896 + BPHYT_RS12745 0.51 -0.3
2,907,897 - BPHYT_RS12745 0.51 -1.0
2,907,897 - BPHYT_RS12745 0.51 +0.4
2,907,897 - BPHYT_RS12745 0.51 -0.2
2,907,926 + BPHYT_RS12745 0.55 -0.6
2,907,927 - BPHYT_RS12745 0.55 -0.5
2,908,190 + +0.8
2,908,190 + +0.6
2,908,190 + +1.3
2,908,191 - -2.3
2,908,191 - +0.9
2,908,191 - -0.5

Or see this region's nucleotide sequence