Experiment: 36hr PHYTO2 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS12480 and BPHYT_RS12485 overlap by 21 nucleotides BPHYT_RS12485 and BPHYT_RS12490 are separated by 32 nucleotides
BPHYT_RS12480: BPHYT_RS12480 - hypothetical protein, at 2,846,224 to 2,847,582
_RS12480
BPHYT_RS12485: BPHYT_RS12485 - peptidyl-prolyl cis-trans isomerase, at 2,847,562 to 2,848,104
_RS12485
BPHYT_RS12490: BPHYT_RS12490 - cupin, at 2,848,137 to 2,849,387
_RS12490
Position (kb)
2847
2848
2849 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2846.695 kb on - strand, within BPHYT_RS12480 at 2846.695 kb on - strand, within BPHYT_RS12480 at 2846.834 kb on - strand, within BPHYT_RS12480 at 2847.222 kb on + strand, within BPHYT_RS12480 at 2847.896 kb on + strand, within BPHYT_RS12485 at 2847.897 kb on - strand, within BPHYT_RS12485 at 2847.995 kb on + strand, within BPHYT_RS12485 at 2847.996 kb on - strand, within BPHYT_RS12485 at 2848.010 kb on + strand, within BPHYT_RS12485 at 2848.010 kb on + strand, within BPHYT_RS12485 at 2848.067 kb on + strand at 2848.068 kb on - strand at 2848.120 kb on - strand at 2848.132 kb on - strand at 2848.133 kb on + strand at 2848.133 kb on + strand at 2848.134 kb on - strand at 2848.134 kb on - strand at 2848.134 kb on - strand at 2848.134 kb on - strand at 2848.421 kb on + strand, within BPHYT_RS12490 at 2848.422 kb on - strand, within BPHYT_RS12490 at 2848.593 kb on + strand, within BPHYT_RS12490 at 2848.635 kb on + strand, within BPHYT_RS12490 at 2848.635 kb on + strand, within BPHYT_RS12490 at 2848.636 kb on - strand, within BPHYT_RS12490 at 2848.636 kb on - strand, within BPHYT_RS12490 at 2848.636 kb on - strand, within BPHYT_RS12490 at 2848.636 kb on - strand, within BPHYT_RS12490 at 2848.658 kb on - strand, within BPHYT_RS12490 at 2848.682 kb on + strand, within BPHYT_RS12490 at 2848.785 kb on + strand, within BPHYT_RS12490 at 2848.785 kb on + strand, within BPHYT_RS12490 at 2848.786 kb on - strand, within BPHYT_RS12490 at 2849.048 kb on + strand, within BPHYT_RS12490 at 2849.048 kb on + strand, within BPHYT_RS12490 at 2849.070 kb on + strand, within BPHYT_RS12490 at 2849.070 kb on + strand, within BPHYT_RS12490 at 2849.070 kb on + strand, within BPHYT_RS12490 at 2849.070 kb on + strand, within BPHYT_RS12490
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO2 MOI 1 remove 2,846,695 - BPHYT_RS12480 0.35 +1.1 2,846,695 - BPHYT_RS12480 0.35 +0.4 2,846,834 - BPHYT_RS12480 0.45 -1.4 2,847,222 + BPHYT_RS12480 0.73 -0.6 2,847,896 + BPHYT_RS12485 0.62 -0.8 2,847,897 - BPHYT_RS12485 0.62 -1.8 2,847,995 + BPHYT_RS12485 0.80 -0.3 2,847,996 - BPHYT_RS12485 0.80 -0.8 2,848,010 + BPHYT_RS12485 0.83 -1.8 2,848,010 + BPHYT_RS12485 0.83 -0.2 2,848,067 + +0.3 2,848,068 - -1.5 2,848,120 - -0.8 2,848,132 - +0.6 2,848,133 + +0.3 2,848,133 + +0.2 2,848,134 - +0.5 2,848,134 - +0.1 2,848,134 - -0.1 2,848,134 - -0.2 2,848,421 + BPHYT_RS12490 0.23 +0.3 2,848,422 - BPHYT_RS12490 0.23 -1.2 2,848,593 + BPHYT_RS12490 0.36 -0.2 2,848,635 + BPHYT_RS12490 0.40 +0.9 2,848,635 + BPHYT_RS12490 0.40 +1.0 2,848,636 - BPHYT_RS12490 0.40 +1.3 2,848,636 - BPHYT_RS12490 0.40 -0.9 2,848,636 - BPHYT_RS12490 0.40 -1.6 2,848,636 - BPHYT_RS12490 0.40 -1.3 2,848,658 - BPHYT_RS12490 0.42 -0.9 2,848,682 + BPHYT_RS12490 0.44 -1.1 2,848,785 + BPHYT_RS12490 0.52 +0.4 2,848,785 + BPHYT_RS12490 0.52 -0.5 2,848,786 - BPHYT_RS12490 0.52 -0.5 2,849,048 + BPHYT_RS12490 0.73 +0.7 2,849,048 + BPHYT_RS12490 0.73 -1.4 2,849,070 + BPHYT_RS12490 0.75 +0.5 2,849,070 + BPHYT_RS12490 0.75 +1.0 2,849,070 + BPHYT_RS12490 0.75 +0.8 2,849,070 + BPHYT_RS12490 0.75 +2.3
Or see this region's nucleotide sequence