Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS12225

Experiment: 36hr PHYTO2 MOI 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS12220 and BPHYT_RS12225 are separated by 343 nucleotidesBPHYT_RS12225 and BPHYT_RS12230 are separated by 222 nucleotidesBPHYT_RS12230 and BPHYT_RS12235 are separated by 143 nucleotides BPHYT_RS12220: BPHYT_RS12220 - glutathione ABC transporter ATP-binding protein, at 2,789,984 to 2,791,936 _RS12220 BPHYT_RS12225: BPHYT_RS12225 - carnitine dehydratase, at 2,792,280 to 2,793,323 _RS12225 BPHYT_RS12230: BPHYT_RS12230 - hypothetical protein, at 2,793,546 to 2,793,824 _RS12230 BPHYT_RS12235: BPHYT_RS12235 - DNA repair protein RadA, at 2,793,968 to 2,795,344 _RS12235 Position (kb) 2792 2793 2794Strain fitness (log2 ratio) -2 -1 0 1 2 3at 2791.470 kb on + strand, within BPHYT_RS12220at 2791.470 kb on + strand, within BPHYT_RS12220at 2791.470 kb on + strand, within BPHYT_RS12220at 2791.470 kb on + strand, within BPHYT_RS12220at 2791.471 kb on - strand, within BPHYT_RS12220at 2792.063 kb on + strandat 2792.064 kb on - strandat 2792.187 kb on - strandat 2792.691 kb on + strand, within BPHYT_RS12225at 2792.692 kb on - strand, within BPHYT_RS12225at 2792.692 kb on - strand, within BPHYT_RS12225at 2792.906 kb on - strand, within BPHYT_RS12225at 2792.974 kb on - strand, within BPHYT_RS12225at 2793.321 kb on + strandat 2793.322 kb on - strandat 2793.604 kb on + strand, within BPHYT_RS12230at 2793.693 kb on + strand, within BPHYT_RS12230at 2793.873 kb on + strandat 2793.894 kb on - strandat 2793.898 kb on + strandat 2793.898 kb on + strandat 2793.915 kb on + strandat 2793.915 kb on + strandat 2793.970 kb on - strandat 2793.970 kb on - strandat 2793.970 kb on - strandat 2794.028 kb on + strandat 2794.028 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 36hr PHYTO2 MOI 1
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2,791,470 + BPHYT_RS12220 0.76 +0.3
2,791,470 + BPHYT_RS12220 0.76 +0.1
2,791,470 + BPHYT_RS12220 0.76 -0.6
2,791,470 + BPHYT_RS12220 0.76 -1.0
2,791,471 - BPHYT_RS12220 0.76 -0.2
2,792,063 + +0.5
2,792,064 - -1.3
2,792,187 - +0.0
2,792,691 + BPHYT_RS12225 0.39 +0.5
2,792,692 - BPHYT_RS12225 0.39 -0.1
2,792,692 - BPHYT_RS12225 0.39 -1.8
2,792,906 - BPHYT_RS12225 0.60 +2.3
2,792,974 - BPHYT_RS12225 0.66 +0.7
2,793,321 + -0.7
2,793,322 - +0.5
2,793,604 + BPHYT_RS12230 0.21 +0.6
2,793,693 + BPHYT_RS12230 0.53 -0.2
2,793,873 + +3.3
2,793,894 - +1.9
2,793,898 + +0.0
2,793,898 + +0.6
2,793,915 + +0.8
2,793,915 + -0.7
2,793,970 - +0.2
2,793,970 - +0.4
2,793,970 - -0.2
2,794,028 + -0.5
2,794,028 + -1.4

Or see this region's nucleotide sequence