Experiment: 36hr PHYTO2 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS12220 and BPHYT_RS12225 are separated by 343 nucleotides BPHYT_RS12225 and BPHYT_RS12230 are separated by 222 nucleotides BPHYT_RS12230 and BPHYT_RS12235 are separated by 143 nucleotides
BPHYT_RS12220: BPHYT_RS12220 - glutathione ABC transporter ATP-binding protein, at 2,789,984 to 2,791,936
_RS12220
BPHYT_RS12225: BPHYT_RS12225 - carnitine dehydratase, at 2,792,280 to 2,793,323
_RS12225
BPHYT_RS12230: BPHYT_RS12230 - hypothetical protein, at 2,793,546 to 2,793,824
_RS12230
BPHYT_RS12235: BPHYT_RS12235 - DNA repair protein RadA, at 2,793,968 to 2,795,344
_RS12235
Position (kb)
2792
2793
2794 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2791.470 kb on + strand, within BPHYT_RS12220 at 2791.470 kb on + strand, within BPHYT_RS12220 at 2791.470 kb on + strand, within BPHYT_RS12220 at 2791.470 kb on + strand, within BPHYT_RS12220 at 2791.471 kb on - strand, within BPHYT_RS12220 at 2792.063 kb on + strand at 2792.064 kb on - strand at 2792.187 kb on - strand at 2792.691 kb on + strand, within BPHYT_RS12225 at 2792.692 kb on - strand, within BPHYT_RS12225 at 2792.692 kb on - strand, within BPHYT_RS12225 at 2792.906 kb on - strand, within BPHYT_RS12225 at 2792.974 kb on - strand, within BPHYT_RS12225 at 2793.321 kb on + strand at 2793.322 kb on - strand at 2793.604 kb on + strand, within BPHYT_RS12230 at 2793.693 kb on + strand, within BPHYT_RS12230 at 2793.873 kb on + strand at 2793.894 kb on - strand at 2793.898 kb on + strand at 2793.898 kb on + strand at 2793.915 kb on + strand at 2793.915 kb on + strand at 2793.970 kb on - strand at 2793.970 kb on - strand at 2793.970 kb on - strand at 2794.028 kb on + strand at 2794.028 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO2 MOI 1 remove 2,791,470 + BPHYT_RS12220 0.76 +0.3 2,791,470 + BPHYT_RS12220 0.76 +0.1 2,791,470 + BPHYT_RS12220 0.76 -0.6 2,791,470 + BPHYT_RS12220 0.76 -1.0 2,791,471 - BPHYT_RS12220 0.76 -0.2 2,792,063 + +0.5 2,792,064 - -1.3 2,792,187 - +0.0 2,792,691 + BPHYT_RS12225 0.39 +0.5 2,792,692 - BPHYT_RS12225 0.39 -0.1 2,792,692 - BPHYT_RS12225 0.39 -1.8 2,792,906 - BPHYT_RS12225 0.60 +2.3 2,792,974 - BPHYT_RS12225 0.66 +0.7 2,793,321 + -0.7 2,793,322 - +0.5 2,793,604 + BPHYT_RS12230 0.21 +0.6 2,793,693 + BPHYT_RS12230 0.53 -0.2 2,793,873 + +3.3 2,793,894 - +1.9 2,793,898 + +0.0 2,793,898 + +0.6 2,793,915 + +0.8 2,793,915 + -0.7 2,793,970 - +0.2 2,793,970 - +0.4 2,793,970 - -0.2 2,794,028 + -0.5 2,794,028 + -1.4
Or see this region's nucleotide sequence