Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS11745

Experiment: 36hr PHYTO2 MOI 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS11740 and BPHYT_RS11745 are separated by 183 nucleotidesBPHYT_RS11745 and BPHYT_RS11750 are separated by 31 nucleotides BPHYT_RS11740: BPHYT_RS11740 - LysR family transcriptional regulator, at 2,662,654 to 2,663,580 _RS11740 BPHYT_RS11745: BPHYT_RS11745 - (2Fe-2S)-binding protein, at 2,663,764 to 2,664,927 _RS11745 BPHYT_RS11750: BPHYT_RS11750 - spermidine/putrescine ABC transporter ATP-binding protein, at 2,664,959 to 2,666,140 _RS11750 Position (kb) 2663 2664 2665Strain fitness (log2 ratio) -1 0 1at 2663.551 kb on + strandat 2663.625 kb on - strandat 2664.242 kb on - strand, within BPHYT_RS11745at 2665.019 kb on + strandat 2665.220 kb on + strand, within BPHYT_RS11750at 2665.536 kb on - strand, within BPHYT_RS11750

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Per-strain Table

Position Strand Gene LocusTag Fraction 36hr PHYTO2 MOI 1
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2,663,551 + +0.7
2,663,625 - +0.3
2,664,242 - BPHYT_RS11745 0.41 -0.3
2,665,019 + -0.3
2,665,220 + BPHYT_RS11750 0.22 +0.2
2,665,536 - BPHYT_RS11750 0.49 -1.3

Or see this region's nucleotide sequence