Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS10695

Experiment: 36hr PHYTO2 MOI 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS10690 and BPHYT_RS10695 overlap by 4 nucleotidesBPHYT_RS10695 and BPHYT_RS10700 are separated by 32 nucleotidesBPHYT_RS10700 and BPHYT_RS10705 are separated by 40 nucleotides BPHYT_RS10690: BPHYT_RS10690 - Rieske (2Fe-2S) protein, at 2,433,692 to 2,434,975 _RS10690 BPHYT_RS10695: BPHYT_RS10695 - aromatic-ring-hydroxylating dioxygenase subunit beta, at 2,434,972 to 2,435,472 _RS10695 BPHYT_RS10700: BPHYT_RS10700 - 4-oxalocrotonate tautomerase, at 2,435,505 to 2,435,888 _RS10700 BPHYT_RS10705: BPHYT_RS10705 - MFS transporter, at 2,435,929 to 2,437,245 _RS10705 Position (kb) 2434 2435 2436Strain fitness (log2 ratio) -1 0 1at 2434.035 kb on - strand, within BPHYT_RS10690at 2434.137 kb on - strand, within BPHYT_RS10690at 2435.108 kb on - strand, within BPHYT_RS10695at 2435.510 kb on + strandat 2435.511 kb on - strandat 2436.430 kb on + strand, within BPHYT_RS10705

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Per-strain Table

Position Strand Gene LocusTag Fraction 36hr PHYTO2 MOI 1
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2,434,035 - BPHYT_RS10690 0.27 -0.4
2,434,137 - BPHYT_RS10690 0.35 +0.1
2,435,108 - BPHYT_RS10695 0.27 -0.2
2,435,510 + +1.0
2,435,511 - +0.0
2,436,430 + BPHYT_RS10705 0.38 -1.4

Or see this region's nucleotide sequence