Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS10625

Experiment: 36hr PHYTO2 MOI 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS10620 and BPHYT_RS10625 are separated by 119 nucleotidesBPHYT_RS10625 and BPHYT_RS10630 are separated by 108 nucleotides BPHYT_RS10620: BPHYT_RS10620 - 2-aminoethylphosphonate:pyruvate aminotransferase, at 2,420,286 to 2,421,518 _RS10620 BPHYT_RS10625: BPHYT_RS10625 - LysR family transcriptional regulator, at 2,421,638 to 2,422,546 _RS10625 BPHYT_RS10630: BPHYT_RS10630 - oxidoreductase, at 2,422,655 to 2,423,401 _RS10630 Position (kb) 2421 2422 2423Strain fitness (log2 ratio) -2 -1 0 1at 2421.053 kb on + strand, within BPHYT_RS10620at 2421.107 kb on - strand, within BPHYT_RS10620at 2421.129 kb on - strand, within BPHYT_RS10620at 2421.175 kb on + strand, within BPHYT_RS10620at 2421.247 kb on + strand, within BPHYT_RS10620at 2421.320 kb on + strand, within BPHYT_RS10620at 2421.561 kb on - strandat 2421.853 kb on + strand, within BPHYT_RS10625at 2422.287 kb on - strand, within BPHYT_RS10625at 2422.489 kb on - strandat 2422.941 kb on + strand, within BPHYT_RS10630at 2422.941 kb on + strand, within BPHYT_RS10630at 2422.942 kb on - strand, within BPHYT_RS10630at 2423.157 kb on + strand, within BPHYT_RS10630at 2423.415 kb on - strandat 2423.495 kb on + strandat 2423.496 kb on - strandat 2423.496 kb on - strandat 2423.500 kb on - strandat 2423.536 kb on - strandat 2423.540 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 36hr PHYTO2 MOI 1
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2,421,053 + BPHYT_RS10620 0.62 -0.3
2,421,107 - BPHYT_RS10620 0.67 +0.9
2,421,129 - BPHYT_RS10620 0.68 +1.4
2,421,175 + BPHYT_RS10620 0.72 +0.5
2,421,247 + BPHYT_RS10620 0.78 -0.4
2,421,320 + BPHYT_RS10620 0.84 +0.5
2,421,561 - +0.8
2,421,853 + BPHYT_RS10625 0.24 -0.6
2,422,287 - BPHYT_RS10625 0.71 +0.1
2,422,489 - +0.0
2,422,941 + BPHYT_RS10630 0.38 -1.6
2,422,941 + BPHYT_RS10630 0.38 -1.6
2,422,942 - BPHYT_RS10630 0.38 -1.4
2,423,157 + BPHYT_RS10630 0.67 +0.3
2,423,415 - -0.6
2,423,495 + -1.9
2,423,496 - +1.0
2,423,496 - -1.6
2,423,500 - +0.8
2,423,536 - -0.7
2,423,540 - -0.4

Or see this region's nucleotide sequence