Experiment: 36hr PHYTO2 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS08685 and BPHYT_RS08690 are separated by 90 nucleotides BPHYT_RS08690 and BPHYT_RS08695 are separated by 157 nucleotides BPHYT_RS08695 and BPHYT_RS08700 are separated by 88 nucleotides BPHYT_RS08700 and BPHYT_RS08705 are separated by 143 nucleotides BPHYT_RS08705 and BPHYT_RS08710 are separated by 430 nucleotides
BPHYT_RS08685: BPHYT_RS08685 - hypothetical protein, at 1,974,732 to 1,974,956
_RS08685
BPHYT_RS08690: BPHYT_RS08690 - hypothetical protein, at 1,975,047 to 1,975,292
_RS08690
BPHYT_RS08695: BPHYT_RS08695 - tRNA-His, at 1,975,450 to 1,975,525
_RS08695
BPHYT_RS08700: BPHYT_RS08700 - tRNA-dihydrouridine synthase A, at 1,975,614 to 1,976,615
_RS08700
BPHYT_RS08705: BPHYT_RS08705 - hypothetical protein, at 1,976,759 to 1,977,064
_RS08705
BPHYT_RS08710: BPHYT_RS08710 - hypothetical protein, at 1,977,495 to 1,979,087
_RS08710
Position (kb) 1975
1976
1977 Strain fitness (log2 ratio) -2
-1
0
1
2 at 1974.677 kb on + strand at 1974.822 kb on - strand, within BPHYT_RS08685 at 1974.861 kb on + strand, within BPHYT_RS08685 at 1974.861 kb on + strand, within BPHYT_RS08685 at 1974.862 kb on - strand, within BPHYT_RS08685 at 1974.862 kb on - strand, within BPHYT_RS08685 at 1974.925 kb on + strand, within BPHYT_RS08685 at 1974.926 kb on - strand, within BPHYT_RS08685 at 1975.104 kb on + strand, within BPHYT_RS08690 at 1975.104 kb on + strand, within BPHYT_RS08690 at 1975.104 kb on + strand, within BPHYT_RS08690 at 1975.104 kb on + strand, within BPHYT_RS08690 at 1975.105 kb on - strand, within BPHYT_RS08690 at 1975.105 kb on - strand, within BPHYT_RS08690 at 1975.435 kb on - strand at 1975.698 kb on - strand at 1975.744 kb on + strand, within BPHYT_RS08700 at 1975.780 kb on + strand, within BPHYT_RS08700 at 1975.781 kb on - strand, within BPHYT_RS08700 at 1975.781 kb on - strand, within BPHYT_RS08700 at 1975.867 kb on + strand, within BPHYT_RS08700 at 1975.867 kb on + strand, within BPHYT_RS08700 at 1976.036 kb on - strand, within BPHYT_RS08700 at 1976.170 kb on + strand, within BPHYT_RS08700 at 1976.171 kb on - strand, within BPHYT_RS08700 at 1976.347 kb on + strand, within BPHYT_RS08700 at 1976.412 kb on + strand, within BPHYT_RS08700 at 1976.412 kb on + strand, within BPHYT_RS08700 at 1976.532 kb on + strand at 1976.685 kb on - strand at 1976.953 kb on + strand, within BPHYT_RS08705 at 1976.953 kb on + strand, within BPHYT_RS08705 at 1976.954 kb on - strand, within BPHYT_RS08705 at 1976.980 kb on + strand, within BPHYT_RS08705 at 1977.011 kb on - strand, within BPHYT_RS08705 at 1977.047 kb on - strand at 1977.059 kb on - strand at 1977.064 kb on + strand at 1977.065 kb on - strand at 1977.070 kb on + strand at 1977.314 kb on + strand at 1977.314 kb on + strand at 1977.314 kb on + strand at 1977.373 kb on - strand at 1977.392 kb on - strand at 1977.483 kb on - strand at 1977.483 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO2 MOI 1 remove 1,974,677 + -0.0 1,974,822 - BPHYT_RS08685 0.40 +0.9 1,974,861 + BPHYT_RS08685 0.57 +0.5 1,974,861 + BPHYT_RS08685 0.57 +0.6 1,974,862 - BPHYT_RS08685 0.58 +0.3 1,974,862 - BPHYT_RS08685 0.58 +0.3 1,974,925 + BPHYT_RS08685 0.86 +0.7 1,974,926 - BPHYT_RS08685 0.86 +0.5 1,975,104 + BPHYT_RS08690 0.23 -1.3 1,975,104 + BPHYT_RS08690 0.23 -0.2 1,975,104 + BPHYT_RS08690 0.23 -1.8 1,975,104 + BPHYT_RS08690 0.23 -0.5 1,975,105 - BPHYT_RS08690 0.24 -0.7 1,975,105 - BPHYT_RS08690 0.24 +1.1 1,975,435 - +1.0 1,975,698 - +0.3 1,975,744 + BPHYT_RS08700 0.13 -0.5 1,975,780 + BPHYT_RS08700 0.17 -0.6 1,975,781 - BPHYT_RS08700 0.17 -1.0 1,975,781 - BPHYT_RS08700 0.17 -2.5 1,975,867 + BPHYT_RS08700 0.25 +0.8 1,975,867 + BPHYT_RS08700 0.25 +1.1 1,976,036 - BPHYT_RS08700 0.42 +1.5 1,976,170 + BPHYT_RS08700 0.55 -0.3 1,976,171 - BPHYT_RS08700 0.56 +0.1 1,976,347 + BPHYT_RS08700 0.73 +0.7 1,976,412 + BPHYT_RS08700 0.80 +1.0 1,976,412 + BPHYT_RS08700 0.80 -1.0 1,976,532 + -0.3 1,976,685 - -0.3 1,976,953 + BPHYT_RS08705 0.63 -0.5 1,976,953 + BPHYT_RS08705 0.63 +0.5 1,976,954 - BPHYT_RS08705 0.64 -0.0 1,976,980 + BPHYT_RS08705 0.72 -2.4 1,977,011 - BPHYT_RS08705 0.82 -1.7 1,977,047 - -1.3 1,977,059 - -1.3 1,977,064 + -0.4 1,977,065 - +0.1 1,977,070 + -1.6 1,977,314 + +0.9 1,977,314 + -1.2 1,977,314 + +0.7 1,977,373 - +1.5 1,977,392 - +0.0 1,977,483 - +0.4 1,977,483 - +2.3
Or see this region's nucleotide sequence