Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS08305

Experiment: 36hr PHYTO2 MOI 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS08300 and BPHYT_RS08305 are separated by 408 nucleotidesBPHYT_RS08305 and BPHYT_RS08310 are separated by 75 nucleotidesBPHYT_RS08310 and BPHYT_RS08315 are separated by 146 nucleotides BPHYT_RS08300: BPHYT_RS08300 - sugar transporter, at 1,898,298 to 1,899,518 _RS08300 BPHYT_RS08305: BPHYT_RS08305 - membrane protein, at 1,899,927 to 1,900,301 _RS08305 BPHYT_RS08310: BPHYT_RS08310 - GNAT family acetyltransferase, at 1,900,377 to 1,900,898 _RS08310 BPHYT_RS08315: BPHYT_RS08315 - chloride channel protein, at 1,901,045 to 1,902,376 _RS08315 Position (kb) 1899 1900 1901Strain fitness (log2 ratio) -2 -1 0 1 2at 1899.043 kb on + strand, within BPHYT_RS08300at 1899.082 kb on + strand, within BPHYT_RS08300at 1899.083 kb on - strand, within BPHYT_RS08300at 1899.083 kb on - strand, within BPHYT_RS08300at 1899.611 kb on - strandat 1899.910 kb on + strandat 1899.922 kb on + strandat 1900.008 kb on + strand, within BPHYT_RS08305at 1900.008 kb on + strand, within BPHYT_RS08305at 1900.008 kb on + strand, within BPHYT_RS08305at 1900.212 kb on + strand, within BPHYT_RS08305at 1900.212 kb on + strand, within BPHYT_RS08305at 1900.225 kb on + strand, within BPHYT_RS08305at 1900.300 kb on - strandat 1900.861 kb on - strandat 1900.861 kb on - strandat 1900.938 kb on - strandat 1901.002 kb on - strandat 1901.022 kb on + strandat 1901.076 kb on + strandat 1901.076 kb on + strandat 1901.196 kb on - strand, within BPHYT_RS08315at 1901.196 kb on - strand, within BPHYT_RS08315at 1901.201 kb on + strand, within BPHYT_RS08315at 1901.202 kb on - strand, within BPHYT_RS08315

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Per-strain Table

Position Strand Gene LocusTag Fraction 36hr PHYTO2 MOI 1
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1,899,043 + BPHYT_RS08300 0.61 +2.3
1,899,082 + BPHYT_RS08300 0.64 -0.3
1,899,083 - BPHYT_RS08300 0.64 +0.5
1,899,083 - BPHYT_RS08300 0.64 +0.6
1,899,611 - +1.3
1,899,910 + -1.5
1,899,922 + +0.2
1,900,008 + BPHYT_RS08305 0.22 +1.2
1,900,008 + BPHYT_RS08305 0.22 -0.4
1,900,008 + BPHYT_RS08305 0.22 +1.9
1,900,212 + BPHYT_RS08305 0.76 +1.4
1,900,212 + BPHYT_RS08305 0.76 -0.5
1,900,225 + BPHYT_RS08305 0.79 -1.0
1,900,300 - -0.7
1,900,861 - +1.1
1,900,861 - +0.2
1,900,938 - -2.0
1,901,002 - -0.9
1,901,022 + -0.9
1,901,076 + -1.3
1,901,076 + +0.6
1,901,196 - BPHYT_RS08315 0.11 -0.7
1,901,196 - BPHYT_RS08315 0.11 -0.5
1,901,201 + BPHYT_RS08315 0.12 +1.4
1,901,202 - BPHYT_RS08315 0.12 -1.2

Or see this region's nucleotide sequence