Experiment: 36hr PHYTO2 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS07315 and BPHYT_RS07320 are separated by 89 nucleotides BPHYT_RS07320 and BPHYT_RS07325 are separated by 50 nucleotides BPHYT_RS07325 and BPHYT_RS07330 are separated by 276 nucleotides
BPHYT_RS07315: BPHYT_RS07315 - lipase, at 1,659,609 to 1,660,565
_RS07315
BPHYT_RS07320: BPHYT_RS07320 - dihydrodipicolinate synthetase, at 1,660,655 to 1,661,563
_RS07320
BPHYT_RS07325: BPHYT_RS07325 - GntR family transcriptional regulator, at 1,661,614 to 1,662,273
_RS07325
BPHYT_RS07330: BPHYT_RS07330 - 5-oxopent-3-ene-1,2,5-tricarboxylate decarboxylase, at 1,662,550 to 1,663,470
_RS07330
Position (kb) 1660
1661
1662 Strain fitness (log2 ratio) -2
-1
0
1
2 at 1659.988 kb on - strand, within BPHYT_RS07315 at 1660.140 kb on + strand, within BPHYT_RS07315 at 1660.140 kb on + strand, within BPHYT_RS07315 at 1660.140 kb on + strand, within BPHYT_RS07315 at 1660.141 kb on - strand, within BPHYT_RS07315 at 1660.141 kb on - strand, within BPHYT_RS07315 at 1660.270 kb on - strand, within BPHYT_RS07315 at 1660.387 kb on - strand, within BPHYT_RS07315 at 1660.490 kb on + strand at 1660.490 kb on + strand at 1660.491 kb on - strand at 1660.616 kb on - strand at 1661.408 kb on - strand, within BPHYT_RS07320 at 1661.907 kb on - strand, within BPHYT_RS07325 at 1662.361 kb on - strand at 1662.476 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO2 MOI 1 remove 1,659,988 - BPHYT_RS07315 0.40 -0.2 1,660,140 + BPHYT_RS07315 0.55 -0.8 1,660,140 + BPHYT_RS07315 0.55 -0.2 1,660,140 + BPHYT_RS07315 0.55 +1.1 1,660,141 - BPHYT_RS07315 0.56 -0.2 1,660,141 - BPHYT_RS07315 0.56 -0.3 1,660,270 - BPHYT_RS07315 0.69 +1.6 1,660,387 - BPHYT_RS07315 0.81 +2.1 1,660,490 + +0.2 1,660,490 + -0.1 1,660,491 - -0.9 1,660,616 - -1.3 1,661,408 - BPHYT_RS07320 0.83 -1.1 1,661,907 - BPHYT_RS07325 0.44 -0.5 1,662,361 - -1.6 1,662,476 + -1.9
Or see this region's nucleotide sequence