Experiment: 36hr PHYTO2 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS05770 and BPHYT_RS05775 are separated by 81 nucleotides BPHYT_RS05775 and BPHYT_RS05780 overlap by 4 nucleotides BPHYT_RS05780 and BPHYT_RS05785 overlap by 4 nucleotides BPHYT_RS05785 and BPHYT_RS05790 are separated by 62 nucleotides
BPHYT_RS05770: BPHYT_RS05770 - peptidase U35, at 1,313,140 to 1,315,152
_RS05770
BPHYT_RS05775: BPHYT_RS05775 - hypothetical protein, at 1,315,234 to 1,315,563
_RS05775
BPHYT_RS05780: BPHYT_RS05780 - hypothetical protein, at 1,315,560 to 1,315,871
_RS05780
BPHYT_RS05785: BPHYT_RS05785 - hypothetical protein, at 1,315,868 to 1,316,299
_RS05785
BPHYT_RS05790: BPHYT_RS05790 - hypothetical protein, at 1,316,362 to 1,317,291
_RS05790
Position (kb) 1315
1316 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 1314.346 kb on + strand, within BPHYT_RS05770 at 1314.473 kb on - strand, within BPHYT_RS05770 at 1314.478 kb on + strand, within BPHYT_RS05770 at 1314.599 kb on - strand, within BPHYT_RS05770 at 1314.640 kb on + strand, within BPHYT_RS05770 at 1314.641 kb on - strand, within BPHYT_RS05770 at 1314.641 kb on - strand, within BPHYT_RS05770 at 1314.641 kb on - strand, within BPHYT_RS05770 at 1314.739 kb on - strand, within BPHYT_RS05770 at 1314.875 kb on - strand, within BPHYT_RS05770 at 1314.875 kb on - strand, within BPHYT_RS05770 at 1315.009 kb on + strand at 1315.040 kb on - strand at 1315.150 kb on + strand at 1315.150 kb on + strand at 1315.151 kb on - strand at 1315.376 kb on - strand, within BPHYT_RS05775 at 1315.393 kb on + strand, within BPHYT_RS05775 at 1315.722 kb on + strand, within BPHYT_RS05780 at 1315.722 kb on + strand, within BPHYT_RS05780 at 1315.735 kb on - strand, within BPHYT_RS05780
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO2 MOI 1 remove 1,314,346 + BPHYT_RS05770 0.60 +0.4 1,314,473 - BPHYT_RS05770 0.66 -0.3 1,314,478 + BPHYT_RS05770 0.66 +0.0 1,314,599 - BPHYT_RS05770 0.72 -0.8 1,314,640 + BPHYT_RS05770 0.75 -0.3 1,314,641 - BPHYT_RS05770 0.75 +0.9 1,314,641 - BPHYT_RS05770 0.75 -0.2 1,314,641 - BPHYT_RS05770 0.75 +0.1 1,314,739 - BPHYT_RS05770 0.79 -0.3 1,314,875 - BPHYT_RS05770 0.86 -1.3 1,314,875 - BPHYT_RS05770 0.86 +0.4 1,315,009 + -1.2 1,315,040 - +0.2 1,315,150 + -1.9 1,315,150 + +3.0 1,315,151 - -0.5 1,315,376 - BPHYT_RS05775 0.43 -0.8 1,315,393 + BPHYT_RS05775 0.48 -1.7 1,315,722 + BPHYT_RS05780 0.52 -0.2 1,315,722 + BPHYT_RS05780 0.52 +0.9 1,315,735 - BPHYT_RS05780 0.56 +1.0
Or see this region's nucleotide sequence