Experiment: 36hr PHYTO2 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS05685 and BPHYT_RS05690 are separated by 20 nucleotides BPHYT_RS05690 and BPHYT_RS05695 are separated by 548 nucleotides BPHYT_RS05695 and BPHYT_RS05700 overlap by 11 nucleotides BPHYT_RS05700 and BPHYT_RS05705 overlap by 11 nucleotides BPHYT_RS05705 and BPHYT_RS05710 overlap by 4 nucleotides
BPHYT_RS05685: BPHYT_RS05685 - hypothetical protein, at 1,298,073 to 1,298,306
_RS05685
BPHYT_RS05690: BPHYT_RS05690 - hypothetical protein, at 1,298,327 to 1,298,698
_RS05690
BPHYT_RS05695: BPHYT_RS05695 - transcriptional regulator, at 1,299,247 to 1,300,137
_RS05695
BPHYT_RS05700: BPHYT_RS05700 - hypothetical protein, at 1,300,127 to 1,300,441
_RS05700
BPHYT_RS05705: BPHYT_RS05705 - hypothetical protein, at 1,300,431 to 1,300,676
_RS05705
BPHYT_RS05710: BPHYT_RS05710 - hypothetical protein, at 1,300,673 to 1,301,020
_RS05710
Position (kb)
1299
1300
1301 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1298.284 kb on + strand at 1298.284 kb on + strand at 1298.285 kb on - strand at 1298.285 kb on - strand at 1298.294 kb on + strand at 1298.298 kb on + strand at 1298.298 kb on + strand at 1298.299 kb on - strand at 1298.299 kb on - strand at 1298.424 kb on + strand, within BPHYT_RS05690 at 1298.743 kb on + strand at 1298.941 kb on - strand at 1299.029 kb on + strand at 1299.177 kb on + strand at 1299.178 kb on - strand at 1299.259 kb on + strand at 1299.361 kb on + strand, within BPHYT_RS05695 at 1299.866 kb on + strand, within BPHYT_RS05695 at 1299.867 kb on - strand, within BPHYT_RS05695 at 1299.872 kb on + strand, within BPHYT_RS05695 at 1299.902 kb on - strand, within BPHYT_RS05695 at 1299.932 kb on + strand, within BPHYT_RS05695 at 1300.194 kb on + strand, within BPHYT_RS05700 at 1300.195 kb on - strand, within BPHYT_RS05700 at 1300.360 kb on + strand, within BPHYT_RS05700 at 1300.360 kb on + strand, within BPHYT_RS05700 at 1300.623 kb on - strand, within BPHYT_RS05705 at 1300.732 kb on + strand, within BPHYT_RS05710 at 1300.851 kb on + strand, within BPHYT_RS05710 at 1300.852 kb on - strand, within BPHYT_RS05710 at 1300.852 kb on - strand, within BPHYT_RS05710 at 1300.852 kb on - strand, within BPHYT_RS05710 at 1300.862 kb on + strand, within BPHYT_RS05710 at 1300.896 kb on + strand, within BPHYT_RS05710 at 1300.896 kb on + strand, within BPHYT_RS05710 at 1300.985 kb on - strand, within BPHYT_RS05710
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO2 MOI 1 remove 1,298,284 + -0.1 1,298,284 + -2.0 1,298,285 - +1.1 1,298,285 - -1.3 1,298,294 + -1.4 1,298,298 + +2.7 1,298,298 + +0.8 1,298,299 - +0.2 1,298,299 - -1.6 1,298,424 + BPHYT_RS05690 0.26 -1.1 1,298,743 + +1.0 1,298,941 - -1.7 1,299,029 + -0.8 1,299,177 + -0.0 1,299,178 - -0.6 1,299,259 + -1.4 1,299,361 + BPHYT_RS05695 0.13 -0.4 1,299,866 + BPHYT_RS05695 0.69 +0.5 1,299,867 - BPHYT_RS05695 0.70 +1.3 1,299,872 + BPHYT_RS05695 0.70 -0.4 1,299,902 - BPHYT_RS05695 0.74 -1.1 1,299,932 + BPHYT_RS05695 0.77 +2.2 1,300,194 + BPHYT_RS05700 0.21 +0.8 1,300,195 - BPHYT_RS05700 0.22 +1.7 1,300,360 + BPHYT_RS05700 0.74 +0.9 1,300,360 + BPHYT_RS05700 0.74 -0.2 1,300,623 - BPHYT_RS05705 0.78 +0.7 1,300,732 + BPHYT_RS05710 0.17 +0.5 1,300,851 + BPHYT_RS05710 0.51 +0.4 1,300,852 - BPHYT_RS05710 0.51 -1.6 1,300,852 - BPHYT_RS05710 0.51 +2.1 1,300,852 - BPHYT_RS05710 0.51 +0.6 1,300,862 + BPHYT_RS05710 0.54 +0.4 1,300,896 + BPHYT_RS05710 0.64 -1.0 1,300,896 + BPHYT_RS05710 0.64 +0.2 1,300,985 - BPHYT_RS05710 0.90 -0.3
Or see this region's nucleotide sequence