Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS04625

Experiment: 36hr PHYTO2 MOI 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS04615 and BPHYT_RS04620 are separated by 117 nucleotidesBPHYT_RS04620 and BPHYT_RS04625 are separated by 32 nucleotidesBPHYT_RS04625 and BPHYT_RS04630 overlap by 4 nucleotides BPHYT_RS04615: BPHYT_RS04615 - urease accessory protein UreD, at 1,068,876 to 1,069,757 _RS04615 BPHYT_RS04620: BPHYT_RS04620 - urea ABC transporter ATP-binding protein, at 1,069,875 to 1,070,567 _RS04620 BPHYT_RS04625: BPHYT_RS04625 - urea ABC transporter ATP-binding protein, at 1,070,600 to 1,071,466 _RS04625 BPHYT_RS04630: BPHYT_RS04630 - amino acid ABC transporter permease, at 1,071,463 to 1,072,647 _RS04630 Position (kb) 1070 1071 1072Strain fitness (log2 ratio) -2 -1 0 1at 1070.094 kb on - strand, within BPHYT_RS04620at 1070.199 kb on - strand, within BPHYT_RS04620at 1070.224 kb on + strand, within BPHYT_RS04620at 1070.225 kb on - strand, within BPHYT_RS04620at 1070.225 kb on - strand, within BPHYT_RS04620at 1070.329 kb on + strand, within BPHYT_RS04620at 1070.330 kb on - strand, within BPHYT_RS04620at 1070.445 kb on - strand, within BPHYT_RS04620at 1070.540 kb on - strandat 1070.540 kb on - strandat 1070.562 kb on + strandat 1070.562 kb on + strandat 1070.614 kb on - strandat 1071.163 kb on - strand, within BPHYT_RS04625at 1071.331 kb on - strand, within BPHYT_RS04625at 1071.569 kb on + strandat 1071.737 kb on + strand, within BPHYT_RS04630at 1071.810 kb on - strand, within BPHYT_RS04630at 1072.286 kb on + strand, within BPHYT_RS04630

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Per-strain Table

Position Strand Gene LocusTag Fraction 36hr PHYTO2 MOI 1
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1,070,094 - BPHYT_RS04620 0.32 +1.4
1,070,199 - BPHYT_RS04620 0.47 -2.0
1,070,224 + BPHYT_RS04620 0.50 -0.2
1,070,225 - BPHYT_RS04620 0.51 +0.5
1,070,225 - BPHYT_RS04620 0.51 +0.2
1,070,329 + BPHYT_RS04620 0.66 +0.8
1,070,330 - BPHYT_RS04620 0.66 -1.0
1,070,445 - BPHYT_RS04620 0.82 -0.4
1,070,540 - -0.4
1,070,540 - -0.1
1,070,562 + -0.7
1,070,562 + -0.9
1,070,614 - -1.3
1,071,163 - BPHYT_RS04625 0.65 -0.5
1,071,331 - BPHYT_RS04625 0.84 +0.1
1,071,569 + +0.3
1,071,737 + BPHYT_RS04630 0.23 -2.1
1,071,810 - BPHYT_RS04630 0.29 -0.7
1,072,286 + BPHYT_RS04630 0.69 -2.4

Or see this region's nucleotide sequence