Experiment: 36hr PHYTO2 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS04615 and BPHYT_RS04620 are separated by 117 nucleotides BPHYT_RS04620 and BPHYT_RS04625 are separated by 32 nucleotides BPHYT_RS04625 and BPHYT_RS04630 overlap by 4 nucleotides
BPHYT_RS04615: BPHYT_RS04615 - urease accessory protein UreD, at 1,068,876 to 1,069,757
_RS04615
BPHYT_RS04620: BPHYT_RS04620 - urea ABC transporter ATP-binding protein, at 1,069,875 to 1,070,567
_RS04620
BPHYT_RS04625: BPHYT_RS04625 - urea ABC transporter ATP-binding protein, at 1,070,600 to 1,071,466
_RS04625
BPHYT_RS04630: BPHYT_RS04630 - amino acid ABC transporter permease, at 1,071,463 to 1,072,647
_RS04630
Position (kb)
1070
1071
1072 Strain fitness (log2 ratio)
-2
-1
0
1 at 1070.094 kb on - strand, within BPHYT_RS04620 at 1070.199 kb on - strand, within BPHYT_RS04620 at 1070.224 kb on + strand, within BPHYT_RS04620 at 1070.225 kb on - strand, within BPHYT_RS04620 at 1070.225 kb on - strand, within BPHYT_RS04620 at 1070.329 kb on + strand, within BPHYT_RS04620 at 1070.330 kb on - strand, within BPHYT_RS04620 at 1070.445 kb on - strand, within BPHYT_RS04620 at 1070.540 kb on - strand at 1070.540 kb on - strand at 1070.562 kb on + strand at 1070.562 kb on + strand at 1070.614 kb on - strand at 1071.163 kb on - strand, within BPHYT_RS04625 at 1071.331 kb on - strand, within BPHYT_RS04625 at 1071.569 kb on + strand at 1071.737 kb on + strand, within BPHYT_RS04630 at 1071.810 kb on - strand, within BPHYT_RS04630 at 1072.286 kb on + strand, within BPHYT_RS04630
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO2 MOI 1 remove 1,070,094 - BPHYT_RS04620 0.32 +1.4 1,070,199 - BPHYT_RS04620 0.47 -2.0 1,070,224 + BPHYT_RS04620 0.50 -0.2 1,070,225 - BPHYT_RS04620 0.51 +0.5 1,070,225 - BPHYT_RS04620 0.51 +0.2 1,070,329 + BPHYT_RS04620 0.66 +0.8 1,070,330 - BPHYT_RS04620 0.66 -1.0 1,070,445 - BPHYT_RS04620 0.82 -0.4 1,070,540 - -0.4 1,070,540 - -0.1 1,070,562 + -0.7 1,070,562 + -0.9 1,070,614 - -1.3 1,071,163 - BPHYT_RS04625 0.65 -0.5 1,071,331 - BPHYT_RS04625 0.84 +0.1 1,071,569 + +0.3 1,071,737 + BPHYT_RS04630 0.23 -2.1 1,071,810 - BPHYT_RS04630 0.29 -0.7 1,072,286 + BPHYT_RS04630 0.69 -2.4
Or see this region's nucleotide sequence