Experiment: 36hr PHYTO2 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS03315 and BPHYT_RS03320 are separated by 87 nucleotides BPHYT_RS03320 and BPHYT_RS03325 are separated by 25 nucleotides BPHYT_RS03325 and BPHYT_RS03330 are separated by 79 nucleotides BPHYT_RS03330 and BPHYT_RS03335 are separated by 44 nucleotides BPHYT_RS03335 and BPHYT_RS03340 are separated by 40 nucleotides
BPHYT_RS03315: BPHYT_RS03315 - polymerase, at 747,186 to 748,307
_RS03315
BPHYT_RS03320: BPHYT_RS03320 - lactoylglutathione lyase, at 748,395 to 748,781
_RS03320
BPHYT_RS03325: BPHYT_RS03325 - hypothetical protein, at 748,807 to 749,205
_RS03325
BPHYT_RS03330: BPHYT_RS03330 - hypothetical protein, at 749,285 to 750,187
_RS03330
BPHYT_RS03335: BPHYT_RS03335 - 1-acyl-sn-glycerol-3-phosphate acyltransferase, at 750,232 to 750,990
_RS03335
BPHYT_RS03340: BPHYT_RS03340 - D,D-heptose 1,7-bisphosphate phosphatase, at 751,031 to 751,585
_RS03340
Position (kb) 749
750
751 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 748.290 kb on - strand at 748.290 kb on - strand at 748.373 kb on - strand at 748.452 kb on + strand, within BPHYT_RS03320 at 748.505 kb on + strand, within BPHYT_RS03320 at 748.591 kb on - strand, within BPHYT_RS03320 at 748.749 kb on + strand at 748.780 kb on - strand at 748.813 kb on - strand at 749.057 kb on - strand, within BPHYT_RS03325 at 749.089 kb on + strand, within BPHYT_RS03325 at 749.130 kb on + strand, within BPHYT_RS03325 at 749.265 kb on + strand at 749.565 kb on + strand, within BPHYT_RS03330 at 749.964 kb on + strand, within BPHYT_RS03330 at 749.965 kb on - strand, within BPHYT_RS03330 at 750.172 kb on + strand at 750.208 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO2 MOI 1 remove 748,290 - +0.9 748,290 - -3.2 748,373 - -1.0 748,452 + BPHYT_RS03320 0.15 +1.3 748,505 + BPHYT_RS03320 0.28 +0.6 748,591 - BPHYT_RS03320 0.51 -0.6 748,749 + +1.3 748,780 - +0.6 748,813 - +1.2 749,057 - BPHYT_RS03325 0.63 -0.2 749,089 + BPHYT_RS03325 0.71 -0.3 749,130 + BPHYT_RS03325 0.81 +0.3 749,265 + -0.8 749,565 + BPHYT_RS03330 0.31 -1.5 749,964 + BPHYT_RS03330 0.75 -1.6 749,965 - BPHYT_RS03330 0.75 +0.8 750,172 + +1.0 750,208 + -2.4
Or see this region's nucleotide sequence