Experiment: 36hr PHYTO2 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS02780 and BPHYT_RS02785 are separated by 42 nucleotides BPHYT_RS02785 and BPHYT_RS02790 are separated by 192 nucleotides BPHYT_RS02790 and BPHYT_RS02795 are separated by 508 nucleotides
BPHYT_RS02780: BPHYT_RS02780 - deacylase, at 624,538 to 625,101
_RS02780
BPHYT_RS02785: BPHYT_RS02785 - hypothetical protein, at 625,144 to 625,527
_RS02785
BPHYT_RS02790: BPHYT_RS02790 - hypothetical protein, at 625,720 to 626,763
_RS02790
BPHYT_RS02795: BPHYT_RS02795 - glycosyl transferase, at 627,272 to 628,243
_RS02795
Position (kb) 625
626
627 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 625.001 kb on - strand, within BPHYT_RS02780 at 625.065 kb on - strand at 625.079 kb on + strand at 625.079 kb on + strand at 625.079 kb on + strand at 625.079 kb on + strand at 625.079 kb on + strand at 625.080 kb on - strand at 625.081 kb on + strand at 625.081 kb on + strand at 625.091 kb on - strand at 625.255 kb on + strand, within BPHYT_RS02785 at 625.256 kb on - strand, within BPHYT_RS02785 at 625.256 kb on - strand, within BPHYT_RS02785 at 625.263 kb on + strand, within BPHYT_RS02785 at 625.264 kb on - strand, within BPHYT_RS02785 at 625.450 kb on + strand, within BPHYT_RS02785 at 625.464 kb on + strand, within BPHYT_RS02785 at 625.464 kb on + strand, within BPHYT_RS02785 at 625.790 kb on + strand at 625.831 kb on + strand, within BPHYT_RS02790 at 625.831 kb on + strand, within BPHYT_RS02790 at 625.832 kb on - strand, within BPHYT_RS02790 at 626.244 kb on - strand, within BPHYT_RS02790 at 626.381 kb on + strand, within BPHYT_RS02790 at 626.381 kb on + strand, within BPHYT_RS02790 at 626.382 kb on - strand, within BPHYT_RS02790 at 626.988 kb on - strand at 627.005 kb on - strand at 627.005 kb on - strand at 627.106 kb on - strand at 627.110 kb on + strand at 627.111 kb on - strand at 627.117 kb on - strand at 627.117 kb on - strand at 627.184 kb on + strand at 627.184 kb on + strand at 627.184 kb on + strand at 627.184 kb on + strand at 627.185 kb on - strand at 627.185 kb on - strand at 627.185 kb on - strand at 627.189 kb on + strand at 627.190 kb on - strand at 627.190 kb on - strand at 627.190 kb on - strand at 627.499 kb on + strand, within BPHYT_RS02795 at 627.611 kb on - strand, within BPHYT_RS02795 at 627.611 kb on - strand, within BPHYT_RS02795 at 627.612 kb on + strand, within BPHYT_RS02795 at 627.612 kb on + strand, within BPHYT_RS02795 at 627.612 kb on + strand, within BPHYT_RS02795 at 627.613 kb on - strand, within BPHYT_RS02795 at 627.613 kb on - strand, within BPHYT_RS02795 at 627.613 kb on - strand, within BPHYT_RS02795
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO2 MOI 1 remove 625,001 - BPHYT_RS02780 0.82 +2.1 625,065 - +0.1 625,079 + -2.9 625,079 + +0.4 625,079 + -0.6 625,079 + -0.1 625,079 + +0.4 625,080 - -0.7 625,081 + -2.0 625,081 + +0.5 625,091 - +0.7 625,255 + BPHYT_RS02785 0.29 -0.9 625,256 - BPHYT_RS02785 0.29 -0.4 625,256 - BPHYT_RS02785 0.29 -0.4 625,263 + BPHYT_RS02785 0.31 -0.5 625,264 - BPHYT_RS02785 0.31 -1.6 625,450 + BPHYT_RS02785 0.80 -0.7 625,464 + BPHYT_RS02785 0.83 -2.0 625,464 + BPHYT_RS02785 0.83 +1.0 625,790 + +0.3 625,831 + BPHYT_RS02790 0.11 +0.4 625,831 + BPHYT_RS02790 0.11 +0.7 625,832 - BPHYT_RS02790 0.11 -0.6 626,244 - BPHYT_RS02790 0.50 +0.4 626,381 + BPHYT_RS02790 0.63 +0.2 626,381 + BPHYT_RS02790 0.63 +0.8 626,382 - BPHYT_RS02790 0.63 -0.2 626,988 - -0.3 627,005 - -1.4 627,005 - -0.8 627,106 - -0.1 627,110 + +0.3 627,111 - +0.3 627,117 - +0.7 627,117 - +0.5 627,184 + -2.0 627,184 + +1.5 627,184 + +0.1 627,184 + +0.2 627,185 - -2.7 627,185 - -3.1 627,185 - +0.3 627,189 + +0.2 627,190 - -0.6 627,190 - +2.7 627,190 - +0.5 627,499 + BPHYT_RS02795 0.23 +0.7 627,611 - BPHYT_RS02795 0.35 +0.1 627,611 - BPHYT_RS02795 0.35 -0.2 627,612 + BPHYT_RS02795 0.35 +0.7 627,612 + BPHYT_RS02795 0.35 +0.4 627,612 + BPHYT_RS02795 0.35 +0.0 627,613 - BPHYT_RS02795 0.35 -0.6 627,613 - BPHYT_RS02795 0.35 +1.3 627,613 - BPHYT_RS02795 0.35 +0.1
Or see this region's nucleotide sequence