Experiment: 36hr PHYTO2 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS01765 and BPHYT_RS01770 are separated by 10 nucleotides BPHYT_RS01770 and BPHYT_RS01775 are separated by 378 nucleotides BPHYT_RS01775 and BPHYT_RS01780 overlap by 4 nucleotides BPHYT_RS01780 and BPHYT_RS01785 are separated by 375 nucleotides
BPHYT_RS01765: BPHYT_RS01765 - carbon-monoxide dehydrogenase, at 401,180 to 403,561
_RS01765
BPHYT_RS01770: BPHYT_RS01770 - carbon monoxide dehydrogenase, at 403,572 to 404,090
_RS01770
BPHYT_RS01775: BPHYT_RS01775 - hypothetical protein, at 404,469 to 404,813
_RS01775
BPHYT_RS01780: BPHYT_RS01780 - hypothetical protein, at 404,810 to 405,178
_RS01780
BPHYT_RS01785: BPHYT_RS01785 - amino acid permease, at 405,554 to 406,945
_RS01785
Position (kb) 404
405 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 403.479 kb on + strand at 403.506 kb on + strand at 403.507 kb on - strand at 403.516 kb on + strand at 403.516 kb on + strand at 403.516 kb on + strand at 403.517 kb on - strand at 404.202 kb on - strand at 404.226 kb on + strand at 404.305 kb on - strand at 404.307 kb on + strand at 404.307 kb on + strand at 404.308 kb on - strand at 404.335 kb on - strand at 404.406 kb on + strand at 404.425 kb on + strand at 404.425 kb on + strand at 404.425 kb on + strand at 404.425 kb on + strand at 404.426 kb on - strand at 404.470 kb on + strand at 404.588 kb on + strand, within BPHYT_RS01775 at 404.589 kb on - strand, within BPHYT_RS01775 at 404.596 kb on + strand, within BPHYT_RS01775 at 404.597 kb on - strand, within BPHYT_RS01775 at 404.705 kb on - strand, within BPHYT_RS01775 at 404.717 kb on + strand, within BPHYT_RS01775 at 404.718 kb on - strand, within BPHYT_RS01775 at 404.720 kb on + strand, within BPHYT_RS01775 at 404.721 kb on - strand, within BPHYT_RS01775 at 404.721 kb on - strand, within BPHYT_RS01775 at 404.820 kb on - strand at 404.867 kb on + strand, within BPHYT_RS01780 at 404.868 kb on - strand, within BPHYT_RS01780 at 404.868 kb on - strand, within BPHYT_RS01780 at 404.868 kb on - strand, within BPHYT_RS01780 at 404.876 kb on - strand, within BPHYT_RS01780 at 405.024 kb on - strand, within BPHYT_RS01780 at 405.144 kb on - strand at 405.146 kb on - strand at 405.214 kb on + strand at 405.214 kb on + strand at 405.284 kb on - strand at 405.325 kb on - strand at 405.373 kb on + strand at 405.373 kb on + strand at 405.527 kb on + strand at 405.797 kb on - strand, within BPHYT_RS01785
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO2 MOI 1 remove 403,479 + +0.0 403,506 + -0.4 403,507 - -1.2 403,516 + -0.7 403,516 + +0.9 403,516 + +0.3 403,517 - +2.8 404,202 - -1.4 404,226 + -0.3 404,305 - -0.1 404,307 + -0.3 404,307 + +0.4 404,308 - +0.4 404,335 - -0.8 404,406 + +0.2 404,425 + +0.0 404,425 + -1.0 404,425 + -1.2 404,425 + +1.2 404,426 - -0.4 404,470 + -0.6 404,588 + BPHYT_RS01775 0.34 -0.3 404,589 - BPHYT_RS01775 0.35 -3.0 404,596 + BPHYT_RS01775 0.37 -0.4 404,597 - BPHYT_RS01775 0.37 -0.4 404,705 - BPHYT_RS01775 0.68 -0.2 404,717 + BPHYT_RS01775 0.72 +0.3 404,718 - BPHYT_RS01775 0.72 -0.1 404,720 + BPHYT_RS01775 0.73 -1.8 404,721 - BPHYT_RS01775 0.73 -1.9 404,721 - BPHYT_RS01775 0.73 -0.8 404,820 - +2.9 404,867 + BPHYT_RS01780 0.15 -0.3 404,868 - BPHYT_RS01780 0.16 -0.4 404,868 - BPHYT_RS01780 0.16 +0.8 404,868 - BPHYT_RS01780 0.16 -0.8 404,876 - BPHYT_RS01780 0.18 +0.0 405,024 - BPHYT_RS01780 0.58 -0.8 405,144 - -0.6 405,146 - +0.1 405,214 + -0.6 405,214 + -2.8 405,284 - +0.2 405,325 - +0.3 405,373 + +0.4 405,373 + +0.2 405,527 + -0.2 405,797 - BPHYT_RS01785 0.17 +0.8
Or see this region's nucleotide sequence