Experiment: 36hr PHYTO2 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS01290 and BPHYT_RS01295 are separated by 61 nucleotides BPHYT_RS01295 and BPHYT_RS01300 are separated by 87 nucleotides BPHYT_RS01300 and BPHYT_RS01305 are separated by 116 nucleotides
BPHYT_RS01290: BPHYT_RS01290 - FAD dependent oxidoreductase, at 294,921 to 296,360
_RS01290
BPHYT_RS01295: BPHYT_RS01295 - glutathione S-transferase, at 296,422 to 297,096
_RS01295
BPHYT_RS01300: BPHYT_RS01300 - ArsR family transcriptional regulator, at 297,184 to 297,879
_RS01300
BPHYT_RS01305: BPHYT_RS01305 - MFS transporter, at 297,996 to 299,387
_RS01305
Position (kb) 297
298 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 296.278 kb on - strand at 296.292 kb on - strand at 296.307 kb on + strand at 296.308 kb on - strand at 296.335 kb on + strand at 296.437 kb on + strand at 296.460 kb on + strand at 296.460 kb on + strand at 296.461 kb on - strand at 296.480 kb on + strand at 296.639 kb on - strand, within BPHYT_RS01295 at 296.747 kb on + strand, within BPHYT_RS01295 at 296.747 kb on + strand, within BPHYT_RS01295 at 296.748 kb on - strand, within BPHYT_RS01295 at 296.819 kb on + strand, within BPHYT_RS01295 at 296.826 kb on + strand, within BPHYT_RS01295 at 297.059 kb on + strand at 297.094 kb on + strand at 297.193 kb on - strand at 297.196 kb on - strand at 297.339 kb on - strand, within BPHYT_RS01300 at 297.542 kb on + strand, within BPHYT_RS01300 at 297.672 kb on - strand, within BPHYT_RS01300 at 297.672 kb on - strand, within BPHYT_RS01300 at 297.811 kb on - strand at 297.864 kb on + strand at 297.864 kb on + strand at 297.864 kb on + strand at 297.865 kb on - strand at 297.897 kb on + strand at 297.907 kb on + strand at 297.976 kb on - strand at 297.993 kb on + strand at 298.045 kb on + strand at 298.082 kb on - strand at 298.085 kb on + strand at 298.085 kb on + strand at 298.085 kb on + strand at 298.086 kb on - strand at 298.086 kb on - strand at 298.139 kb on + strand, within BPHYT_RS01305 at 298.171 kb on - strand, within BPHYT_RS01305 at 298.173 kb on - strand, within BPHYT_RS01305 at 298.173 kb on - strand, within BPHYT_RS01305 at 298.382 kb on + strand, within BPHYT_RS01305 at 298.382 kb on + strand, within BPHYT_RS01305 at 298.382 kb on + strand, within BPHYT_RS01305 at 298.382 kb on + strand, within BPHYT_RS01305 at 298.538 kb on - strand, within BPHYT_RS01305 at 298.875 kb on + strand, within BPHYT_RS01305
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO2 MOI 1 remove 296,278 - -0.8 296,292 - -0.3 296,307 + +0.3 296,308 - -0.3 296,335 + -0.4 296,437 + -0.3 296,460 + -1.0 296,460 + -0.2 296,461 - -0.2 296,480 + +0.4 296,639 - BPHYT_RS01295 0.32 -0.6 296,747 + BPHYT_RS01295 0.48 +0.6 296,747 + BPHYT_RS01295 0.48 +1.1 296,748 - BPHYT_RS01295 0.48 +0.4 296,819 + BPHYT_RS01295 0.59 -0.6 296,826 + BPHYT_RS01295 0.60 -0.6 297,059 + -0.3 297,094 + +4.3 297,193 - -0.4 297,196 - +0.1 297,339 - BPHYT_RS01300 0.22 -1.1 297,542 + BPHYT_RS01300 0.51 -1.6 297,672 - BPHYT_RS01300 0.70 -0.3 297,672 - BPHYT_RS01300 0.70 +0.8 297,811 - -0.7 297,864 + -1.6 297,864 + +0.8 297,864 + +0.2 297,865 - +0.1 297,897 + -1.2 297,907 + +2.4 297,976 - +0.8 297,993 + +0.8 298,045 + +0.0 298,082 - +0.6 298,085 + +1.0 298,085 + -0.3 298,085 + -0.1 298,086 - +0.1 298,086 - +0.1 298,139 + BPHYT_RS01305 0.10 +2.0 298,171 - BPHYT_RS01305 0.13 +2.4 298,173 - BPHYT_RS01305 0.13 +0.3 298,173 - BPHYT_RS01305 0.13 +0.1 298,382 + BPHYT_RS01305 0.28 -1.0 298,382 + BPHYT_RS01305 0.28 +1.2 298,382 + BPHYT_RS01305 0.28 -3.1 298,382 + BPHYT_RS01305 0.28 +0.3 298,538 - BPHYT_RS01305 0.39 -0.6 298,875 + BPHYT_RS01305 0.63 -0.6
Or see this region's nucleotide sequence