Experiment: 36hr PHYTO2 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS01280 and BPHYT_RS01285 are separated by 663 nucleotides BPHYT_RS01285 and BPHYT_RS01290 are separated by 107 nucleotides
BPHYT_RS01280: BPHYT_RS01280 - hypothetical protein, at 293,506 to 293,739
_RS01280
BPHYT_RS01285: BPHYT_RS01285 - polyketide cyclase, at 294,403 to 294,813
_RS01285
BPHYT_RS01290: BPHYT_RS01290 - FAD dependent oxidoreductase, at 294,921 to 296,360
_RS01290
Position (kb) 294
295 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 293.460 kb on + strand at 293.489 kb on + strand at 293.687 kb on - strand, within BPHYT_RS01280 at 293.779 kb on + strand at 293.780 kb on - strand at 294.040 kb on + strand at 294.067 kb on - strand at 294.166 kb on + strand at 294.167 kb on - strand at 294.183 kb on - strand at 294.210 kb on - strand at 294.301 kb on + strand at 294.302 kb on - strand at 294.306 kb on - strand at 294.337 kb on - strand at 294.383 kb on + strand at 294.383 kb on + strand at 294.384 kb on - strand at 294.384 kb on - strand at 294.384 kb on - strand at 294.384 kb on - strand at 294.384 kb on - strand at 294.384 kb on - strand at 294.439 kb on + strand at 294.439 kb on + strand at 294.494 kb on + strand, within BPHYT_RS01285 at 294.494 kb on + strand, within BPHYT_RS01285 at 294.496 kb on + strand, within BPHYT_RS01285 at 294.497 kb on - strand, within BPHYT_RS01285 at 294.497 kb on - strand, within BPHYT_RS01285 at 294.497 kb on - strand at 294.541 kb on + strand, within BPHYT_RS01285 at 294.542 kb on - strand, within BPHYT_RS01285 at 294.640 kb on + strand at 294.776 kb on - strand at 294.841 kb on + strand at 294.880 kb on - strand at 294.892 kb on - strand at 294.892 kb on - strand at 295.094 kb on + strand, within BPHYT_RS01290 at 295.094 kb on + strand, within BPHYT_RS01290 at 295.095 kb on - strand, within BPHYT_RS01290 at 295.097 kb on + strand, within BPHYT_RS01290 at 295.097 kb on + strand, within BPHYT_RS01290 at 295.098 kb on - strand, within BPHYT_RS01290 at 295.152 kb on + strand, within BPHYT_RS01290 at 295.153 kb on - strand, within BPHYT_RS01290 at 295.218 kb on + strand, within BPHYT_RS01290 at 295.284 kb on - strand, within BPHYT_RS01290 at 295.285 kb on + strand, within BPHYT_RS01290 at 295.285 kb on + strand, within BPHYT_RS01290 at 295.286 kb on - strand, within BPHYT_RS01290 at 295.286 kb on - strand, within BPHYT_RS01290 at 295.336 kb on + strand, within BPHYT_RS01290 at 295.353 kb on - strand, within BPHYT_RS01290 at 295.353 kb on - strand, within BPHYT_RS01290 at 295.486 kb on - strand, within BPHYT_RS01290 at 295.486 kb on - strand, within BPHYT_RS01290
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO2 MOI 1 remove 293,460 + +1.3 293,489 + +0.8 293,687 - BPHYT_RS01280 0.77 -1.6 293,779 + -0.4 293,780 - +0.4 294,040 + -0.8 294,067 - -1.3 294,166 + -1.2 294,167 - +0.7 294,183 - +1.7 294,210 - -1.9 294,301 + +0.2 294,302 - +0.0 294,306 - -0.4 294,337 - -1.8 294,383 + +1.4 294,383 + -0.9 294,384 - -0.4 294,384 - +0.1 294,384 - +0.1 294,384 - -0.1 294,384 - -0.3 294,384 - -0.5 294,439 + +3.4 294,439 + -1.0 294,494 + BPHYT_RS01285 0.22 -0.7 294,494 + BPHYT_RS01285 0.22 -1.6 294,496 + BPHYT_RS01285 0.23 -1.6 294,497 - BPHYT_RS01285 0.23 +0.6 294,497 - BPHYT_RS01285 0.23 +0.4 294,497 - +0.6 294,541 + BPHYT_RS01285 0.34 -0.7 294,542 - BPHYT_RS01285 0.34 -0.1 294,640 + +0.4 294,776 - -2.6 294,841 + -1.6 294,880 - -1.5 294,892 - -0.1 294,892 - -0.9 295,094 + BPHYT_RS01290 0.12 +0.1 295,094 + BPHYT_RS01290 0.12 +0.9 295,095 - BPHYT_RS01290 0.12 +0.2 295,097 + BPHYT_RS01290 0.12 -1.9 295,097 + BPHYT_RS01290 0.12 -0.6 295,098 - BPHYT_RS01290 0.12 -1.7 295,152 + BPHYT_RS01290 0.16 -2.4 295,153 - BPHYT_RS01290 0.16 +0.1 295,218 + BPHYT_RS01290 0.21 +0.4 295,284 - BPHYT_RS01290 0.25 +0.1 295,285 + BPHYT_RS01290 0.25 -1.6 295,285 + BPHYT_RS01290 0.25 +0.2 295,286 - BPHYT_RS01290 0.25 +0.3 295,286 - BPHYT_RS01290 0.25 -1.3 295,336 + BPHYT_RS01290 0.29 -0.8 295,353 - BPHYT_RS01290 0.30 +0.1 295,353 - BPHYT_RS01290 0.30 +1.2 295,486 - BPHYT_RS01290 0.39 -0.3 295,486 - BPHYT_RS01290 0.39 +0.0
Or see this region's nucleotide sequence