Experiment: 36hr PHYTO2 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS01275 and BPHYT_RS01280 are separated by 254 nucleotides BPHYT_RS01280 and BPHYT_RS01285 are separated by 663 nucleotides
BPHYT_RS01275: BPHYT_RS01275 - DNA-directed DNA polymerase, at 292,094 to 293,251
_RS01275
BPHYT_RS01280: BPHYT_RS01280 - hypothetical protein, at 293,506 to 293,739
_RS01280
BPHYT_RS01285: BPHYT_RS01285 - polyketide cyclase, at 294,403 to 294,813
_RS01285
Position (kb) 293
294 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 292.534 kb on - strand, within BPHYT_RS01275 at 292.534 kb on - strand, within BPHYT_RS01275 at 292.800 kb on - strand, within BPHYT_RS01275 at 292.863 kb on + strand, within BPHYT_RS01275 at 292.936 kb on - strand, within BPHYT_RS01275 at 292.936 kb on - strand, within BPHYT_RS01275 at 293.044 kb on - strand, within BPHYT_RS01275 at 293.076 kb on + strand, within BPHYT_RS01275 at 293.077 kb on - strand, within BPHYT_RS01275 at 293.338 kb on + strand at 293.339 kb on - strand at 293.340 kb on + strand at 293.340 kb on + strand at 293.341 kb on - strand at 293.341 kb on - strand at 293.354 kb on - strand at 293.354 kb on - strand at 293.460 kb on + strand at 293.489 kb on + strand at 293.687 kb on - strand, within BPHYT_RS01280 at 293.779 kb on + strand at 293.780 kb on - strand at 294.040 kb on + strand at 294.067 kb on - strand at 294.166 kb on + strand at 294.167 kb on - strand at 294.183 kb on - strand at 294.210 kb on - strand at 294.301 kb on + strand at 294.302 kb on - strand at 294.306 kb on - strand at 294.337 kb on - strand at 294.383 kb on + strand at 294.383 kb on + strand at 294.384 kb on - strand at 294.384 kb on - strand at 294.384 kb on - strand at 294.384 kb on - strand at 294.384 kb on - strand at 294.384 kb on - strand at 294.439 kb on + strand at 294.439 kb on + strand at 294.494 kb on + strand, within BPHYT_RS01285 at 294.494 kb on + strand, within BPHYT_RS01285 at 294.496 kb on + strand, within BPHYT_RS01285 at 294.497 kb on - strand, within BPHYT_RS01285 at 294.497 kb on - strand, within BPHYT_RS01285 at 294.497 kb on - strand at 294.541 kb on + strand, within BPHYT_RS01285 at 294.542 kb on - strand, within BPHYT_RS01285 at 294.640 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO2 MOI 1 remove 292,534 - BPHYT_RS01275 0.38 +1.3 292,534 - BPHYT_RS01275 0.38 +3.4 292,800 - BPHYT_RS01275 0.61 -0.5 292,863 + BPHYT_RS01275 0.66 -0.5 292,936 - BPHYT_RS01275 0.73 +1.4 292,936 - BPHYT_RS01275 0.73 -0.0 293,044 - BPHYT_RS01275 0.82 -0.9 293,076 + BPHYT_RS01275 0.85 +0.8 293,077 - BPHYT_RS01275 0.85 -0.1 293,338 + +1.0 293,339 - -1.3 293,340 + -0.4 293,340 + +1.7 293,341 - +1.3 293,341 - +1.5 293,354 - +0.6 293,354 - +0.5 293,460 + +1.3 293,489 + +0.8 293,687 - BPHYT_RS01280 0.77 -1.6 293,779 + -0.4 293,780 - +0.4 294,040 + -0.8 294,067 - -1.3 294,166 + -1.2 294,167 - +0.7 294,183 - +1.7 294,210 - -1.9 294,301 + +0.2 294,302 - +0.0 294,306 - -0.4 294,337 - -1.8 294,383 + +1.4 294,383 + -0.9 294,384 - -0.4 294,384 - +0.1 294,384 - +0.1 294,384 - -0.1 294,384 - -0.3 294,384 - -0.5 294,439 + +3.4 294,439 + -1.0 294,494 + BPHYT_RS01285 0.22 -0.7 294,494 + BPHYT_RS01285 0.22 -1.6 294,496 + BPHYT_RS01285 0.23 -1.6 294,497 - BPHYT_RS01285 0.23 +0.6 294,497 - BPHYT_RS01285 0.23 +0.4 294,497 - +0.6 294,541 + BPHYT_RS01285 0.34 -0.7 294,542 - BPHYT_RS01285 0.34 -0.1 294,640 + +0.4
Or see this region's nucleotide sequence