Experiment: 36hr PHYTO2 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS00640 and BPHYT_RS00645 are separated by 25 nucleotides BPHYT_RS00645 and BPHYT_RS00650 overlap by 4 nucleotides
BPHYT_RS00640: BPHYT_RS00640 - acyl-CoA dehydrogenase, at 151,596 to 152,684
_RS00640
BPHYT_RS00645: BPHYT_RS00645 - diaminopimelate decarboxylase, at 152,710 to 153,918
_RS00645
BPHYT_RS00650: BPHYT_RS00650 - acyl-CoA ligase, at 153,915 to 155,534
_RS00650
Position (kb) 152
153
154 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4 at 151.783 kb on - strand, within BPHYT_RS00640 at 151.833 kb on + strand, within BPHYT_RS00640 at 151.833 kb on + strand at 151.833 kb on + strand, within BPHYT_RS00640 at 151.834 kb on - strand, within BPHYT_RS00640 at 151.933 kb on + strand, within BPHYT_RS00640 at 151.990 kb on + strand, within BPHYT_RS00640 at 151.990 kb on + strand, within BPHYT_RS00640 at 151.991 kb on - strand, within BPHYT_RS00640 at 152.070 kb on - strand, within BPHYT_RS00640 at 152.099 kb on - strand, within BPHYT_RS00640 at 152.099 kb on - strand, within BPHYT_RS00640 at 152.144 kb on - strand, within BPHYT_RS00640 at 152.166 kb on + strand, within BPHYT_RS00640 at 152.309 kb on - strand, within BPHYT_RS00640 at 152.309 kb on - strand, within BPHYT_RS00640 at 152.318 kb on + strand, within BPHYT_RS00640 at 152.319 kb on - strand, within BPHYT_RS00640 at 152.351 kb on - strand, within BPHYT_RS00640 at 152.650 kb on - strand at 152.650 kb on - strand at 152.880 kb on - strand, within BPHYT_RS00645 at 152.897 kb on - strand, within BPHYT_RS00645 at 152.897 kb on - strand, within BPHYT_RS00645 at 153.050 kb on + strand, within BPHYT_RS00645 at 153.050 kb on + strand, within BPHYT_RS00645 at 153.050 kb on + strand, within BPHYT_RS00645 at 153.050 kb on + strand, within BPHYT_RS00645 at 153.050 kb on + strand, within BPHYT_RS00645 at 153.050 kb on + strand, within BPHYT_RS00645 at 153.050 kb on + strand, within BPHYT_RS00645 at 153.051 kb on - strand, within BPHYT_RS00645 at 153.051 kb on - strand, within BPHYT_RS00645 at 153.068 kb on - strand, within BPHYT_RS00645 at 153.074 kb on + strand, within BPHYT_RS00645 at 153.075 kb on - strand, within BPHYT_RS00645 at 153.229 kb on + strand, within BPHYT_RS00645 at 153.229 kb on + strand, within BPHYT_RS00645 at 153.230 kb on - strand, within BPHYT_RS00645 at 153.707 kb on + strand, within BPHYT_RS00645 at 153.819 kb on - strand at 153.870 kb on + strand at 154.030 kb on + strand at 154.040 kb on + strand at 154.057 kb on + strand at 154.057 kb on + strand at 154.057 kb on + strand at 154.058 kb on - strand at 154.058 kb on - strand at 154.128 kb on + strand, within BPHYT_RS00650 at 154.128 kb on + strand, within BPHYT_RS00650 at 154.129 kb on - strand, within BPHYT_RS00650 at 154.279 kb on + strand, within BPHYT_RS00650 at 154.591 kb on + strand, within BPHYT_RS00650 at 154.591 kb on + strand, within BPHYT_RS00650 at 154.592 kb on - strand, within BPHYT_RS00650 at 154.667 kb on + strand, within BPHYT_RS00650 at 154.668 kb on - strand, within BPHYT_RS00650 at 154.678 kb on + strand, within BPHYT_RS00650 at 154.722 kb on - strand, within BPHYT_RS00650
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO2 MOI 1 remove 151,783 - BPHYT_RS00640 0.17 +1.9 151,833 + BPHYT_RS00640 0.22 +1.5 151,833 + +1.7 151,833 + BPHYT_RS00640 0.22 +0.5 151,834 - BPHYT_RS00640 0.22 +1.6 151,933 + BPHYT_RS00640 0.31 -0.2 151,990 + BPHYT_RS00640 0.36 -0.8 151,990 + BPHYT_RS00640 0.36 +0.3 151,991 - BPHYT_RS00640 0.36 -0.1 152,070 - BPHYT_RS00640 0.44 -0.5 152,099 - BPHYT_RS00640 0.46 -0.2 152,099 - BPHYT_RS00640 0.46 -0.9 152,144 - BPHYT_RS00640 0.50 -0.5 152,166 + BPHYT_RS00640 0.52 -1.4 152,309 - BPHYT_RS00640 0.65 -2.2 152,309 - BPHYT_RS00640 0.65 -0.5 152,318 + BPHYT_RS00640 0.66 +0.5 152,319 - BPHYT_RS00640 0.66 +1.5 152,351 - BPHYT_RS00640 0.69 -2.0 152,650 - -0.3 152,650 - +0.5 152,880 - BPHYT_RS00645 0.14 +0.1 152,897 - BPHYT_RS00645 0.15 -0.2 152,897 - BPHYT_RS00645 0.15 -0.6 153,050 + BPHYT_RS00645 0.28 -0.7 153,050 + BPHYT_RS00645 0.28 +0.2 153,050 + BPHYT_RS00645 0.28 -0.3 153,050 + BPHYT_RS00645 0.28 +0.1 153,050 + BPHYT_RS00645 0.28 -0.0 153,050 + BPHYT_RS00645 0.28 -0.6 153,050 + BPHYT_RS00645 0.28 +0.0 153,051 - BPHYT_RS00645 0.28 +0.4 153,051 - BPHYT_RS00645 0.28 -1.3 153,068 - BPHYT_RS00645 0.30 +0.8 153,074 + BPHYT_RS00645 0.30 -1.3 153,075 - BPHYT_RS00645 0.30 +0.3 153,229 + BPHYT_RS00645 0.43 -0.3 153,229 + BPHYT_RS00645 0.43 -1.7 153,230 - BPHYT_RS00645 0.43 -0.3 153,707 + BPHYT_RS00645 0.82 -2.1 153,819 - +0.3 153,870 + +4.7 154,030 + -0.7 154,040 + -0.0 154,057 + -0.5 154,057 + +0.0 154,057 + -0.1 154,058 - -1.8 154,058 - -0.5 154,128 + BPHYT_RS00650 0.13 +0.1 154,128 + BPHYT_RS00650 0.13 -1.1 154,129 - BPHYT_RS00650 0.13 -0.6 154,279 + BPHYT_RS00650 0.22 +0.2 154,591 + BPHYT_RS00650 0.42 +0.3 154,591 + BPHYT_RS00650 0.42 +1.0 154,592 - BPHYT_RS00650 0.42 -1.1 154,667 + BPHYT_RS00650 0.46 -0.4 154,668 - BPHYT_RS00650 0.46 +0.3 154,678 + BPHYT_RS00650 0.47 +0.1 154,722 - BPHYT_RS00650 0.50 -0.3
Or see this region's nucleotide sequence