Experiment: 36hr PHYTO2 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS00390 and BPHYT_RS00395 are separated by 259 nucleotides BPHYT_RS00395 and BPHYT_RS00400 are separated by 510 nucleotides BPHYT_RS00400 and BPHYT_RS00405 are separated by 17 nucleotides
BPHYT_RS00390: BPHYT_RS00390 - AraC family transcriptional regulator, at 97,797 to 98,831
_RS00390
BPHYT_RS00395: BPHYT_RS00395 - aldehyde dehydrogenase, at 99,091 to 100,518
_RS00395
BPHYT_RS00400: BPHYT_RS00400 - hypothetical protein, at 101,029 to 101,316
_RS00400
BPHYT_RS00405: BPHYT_RS00405 - hypothetical protein, at 101,334 to 101,687
_RS00405
Position (kb) 99
100
101 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 98.176 kb on - strand, within BPHYT_RS00390 at 98.235 kb on + strand, within BPHYT_RS00390 at 98.409 kb on + strand, within BPHYT_RS00390 at 98.434 kb on + strand, within BPHYT_RS00390 at 98.498 kb on - strand, within BPHYT_RS00390 at 98.498 kb on - strand, within BPHYT_RS00390 at 98.659 kb on - strand, within BPHYT_RS00390 at 98.990 kb on + strand at 98.991 kb on - strand at 99.014 kb on - strand at 99.069 kb on + strand at 99.070 kb on - strand at 99.099 kb on + strand at 99.351 kb on - strand, within BPHYT_RS00395 at 99.351 kb on - strand, within BPHYT_RS00395 at 99.360 kb on + strand, within BPHYT_RS00395 at 99.393 kb on - strand at 99.447 kb on + strand, within BPHYT_RS00395 at 99.452 kb on - strand, within BPHYT_RS00395 at 99.859 kb on + strand, within BPHYT_RS00395 at 100.250 kb on - strand, within BPHYT_RS00395 at 100.461 kb on - strand at 100.579 kb on - strand at 100.681 kb on - strand at 100.985 kb on - strand at 101.083 kb on - strand, within BPHYT_RS00400 at 101.132 kb on + strand, within BPHYT_RS00400 at 101.133 kb on - strand, within BPHYT_RS00400 at 101.177 kb on - strand, within BPHYT_RS00400 at 101.308 kb on - strand at 101.339 kb on + strand at 101.340 kb on - strand at 101.340 kb on - strand at 101.371 kb on + strand, within BPHYT_RS00405 at 101.372 kb on - strand, within BPHYT_RS00405 at 101.372 kb on - strand, within BPHYT_RS00405 at 101.373 kb on + strand, within BPHYT_RS00405 at 101.373 kb on + strand, within BPHYT_RS00405 at 101.373 kb on + strand, within BPHYT_RS00405 at 101.373 kb on + strand, within BPHYT_RS00405 at 101.374 kb on - strand, within BPHYT_RS00405 at 101.374 kb on - strand, within BPHYT_RS00405 at 101.374 kb on - strand, within BPHYT_RS00405 at 101.513 kb on + strand, within BPHYT_RS00405 at 101.513 kb on + strand, within BPHYT_RS00405 at 101.514 kb on - strand, within BPHYT_RS00405
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO2 MOI 1 remove 98,176 - BPHYT_RS00390 0.37 +0.8 98,235 + BPHYT_RS00390 0.42 +1.8 98,409 + BPHYT_RS00390 0.59 +0.5 98,434 + BPHYT_RS00390 0.62 -1.0 98,498 - BPHYT_RS00390 0.68 +0.4 98,498 - BPHYT_RS00390 0.68 +1.9 98,659 - BPHYT_RS00390 0.83 +0.3 98,990 + -0.2 98,991 - +1.2 99,014 - +1.2 99,069 + +0.7 99,070 - -0.5 99,099 + -1.9 99,351 - BPHYT_RS00395 0.18 +0.2 99,351 - BPHYT_RS00395 0.18 +0.1 99,360 + BPHYT_RS00395 0.19 -0.3 99,393 - -0.0 99,447 + BPHYT_RS00395 0.25 +0.0 99,452 - BPHYT_RS00395 0.25 -0.1 99,859 + BPHYT_RS00395 0.54 +1.5 100,250 - BPHYT_RS00395 0.81 -1.1 100,461 - +0.6 100,579 - +1.0 100,681 - +0.4 100,985 - +0.2 101,083 - BPHYT_RS00400 0.19 -0.8 101,132 + BPHYT_RS00400 0.36 +0.1 101,133 - BPHYT_RS00400 0.36 -0.8 101,177 - BPHYT_RS00400 0.51 -0.6 101,308 - -0.7 101,339 + -2.3 101,340 - -1.5 101,340 - -0.9 101,371 + BPHYT_RS00405 0.10 +0.1 101,372 - BPHYT_RS00405 0.11 +0.1 101,372 - BPHYT_RS00405 0.11 +0.6 101,373 + BPHYT_RS00405 0.11 -2.1 101,373 + BPHYT_RS00405 0.11 -0.9 101,373 + BPHYT_RS00405 0.11 -0.3 101,373 + BPHYT_RS00405 0.11 +0.4 101,374 - BPHYT_RS00405 0.11 +0.3 101,374 - BPHYT_RS00405 0.11 -3.0 101,374 - BPHYT_RS00405 0.11 -0.7 101,513 + BPHYT_RS00405 0.51 -0.4 101,513 + BPHYT_RS00405 0.51 -1.0 101,514 - BPHYT_RS00405 0.51 +0.0
Or see this region's nucleotide sequence