Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS00075

Experiment: 36hr PHYTO2 MOI 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS00070 and BPHYT_RS00075 overlap by 4 nucleotidesBPHYT_RS00075 and BPHYT_RS00080 overlap by 8 nucleotidesBPHYT_RS00080 and BPHYT_RS00085 are separated by 2 nucleotides BPHYT_RS00070: BPHYT_RS00070 - hypothetical protein, at 19,944 to 21,122 _RS00070 BPHYT_RS00075: BPHYT_RS00075 - hypothetical protein, at 21,119 to 24,493 _RS00075 BPHYT_RS00080: BPHYT_RS00080 - hypothetical protein, at 24,486 to 25,133 _RS00080 BPHYT_RS00085: BPHYT_RS00085 - hypothetical protein, at 25,136 to 26,590 _RS00085 Position (kb) 21 22 23 24 25Strain fitness (log2 ratio) -2 -1 0 1 2 3 4at 20.355 kb on - strand, within BPHYT_RS00070at 20.475 kb on - strand, within BPHYT_RS00070at 20.478 kb on + strand, within BPHYT_RS00070at 20.478 kb on + strand, within BPHYT_RS00070at 20.478 kb on + strand, within BPHYT_RS00070at 20.478 kb on + strand, within BPHYT_RS00070at 20.594 kb on - strand, within BPHYT_RS00070at 20.640 kb on + strand, within BPHYT_RS00070at 20.640 kb on + strand, within BPHYT_RS00070at 20.640 kb on + strand, within BPHYT_RS00070at 20.641 kb on - strand, within BPHYT_RS00070at 20.641 kb on - strand, within BPHYT_RS00070at 20.642 kb on + strand, within BPHYT_RS00070at 20.643 kb on - strand, within BPHYT_RS00070at 20.643 kb on - strand, within BPHYT_RS00070at 20.644 kb on + strand, within BPHYT_RS00070at 20.644 kb on + strand, within BPHYT_RS00070at 20.645 kb on - strand, within BPHYT_RS00070at 20.645 kb on - strand, within BPHYT_RS00070at 20.682 kb on + strand, within BPHYT_RS00070at 20.682 kb on + strand, within BPHYT_RS00070at 20.683 kb on - strand, within BPHYT_RS00070at 20.683 kb on - strand, within BPHYT_RS00070at 20.683 kb on - strand, within BPHYT_RS00070at 20.683 kb on - strand, within BPHYT_RS00070at 20.683 kb on - strand, within BPHYT_RS00070at 20.683 kb on - strand, within BPHYT_RS00070at 20.683 kb on - strand, within BPHYT_RS00070at 20.876 kb on + strand, within BPHYT_RS00070at 20.876 kb on + strand, within BPHYT_RS00070at 21.041 kb on + strandat 21.042 kb on - strandat 21.222 kb on - strandat 21.240 kb on + strandat 21.240 kb on + strandat 21.241 kb on - strandat 21.241 kb on - strandat 21.378 kb on - strandat 21.430 kb on + strandat 21.430 kb on + strandat 21.430 kb on + strandat 21.431 kb on - strandat 21.431 kb on - strandat 21.431 kb on - strandat 21.433 kb on - strandat 21.480 kb on + strand, within BPHYT_RS00075at 21.758 kb on - strand, within BPHYT_RS00075at 21.759 kb on + strand, within BPHYT_RS00075at 21.760 kb on - strand, within BPHYT_RS00075at 21.774 kb on + strand, within BPHYT_RS00075at 21.822 kb on - strand, within BPHYT_RS00075at 21.898 kb on + strand, within BPHYT_RS00075at 22.195 kb on - strand, within BPHYT_RS00075at 22.195 kb on - strand, within BPHYT_RS00075at 22.246 kb on + strand, within BPHYT_RS00075at 22.247 kb on - strand, within BPHYT_RS00075at 22.534 kb on + strand, within BPHYT_RS00075at 22.612 kb on + strand, within BPHYT_RS00075at 22.613 kb on - strand, within BPHYT_RS00075at 22.632 kb on + strand, within BPHYT_RS00075at 22.632 kb on + strand, within BPHYT_RS00075at 22.800 kb on + strand, within BPHYT_RS00075at 22.801 kb on - strand, within BPHYT_RS00075at 22.801 kb on - strand, within BPHYT_RS00075at 22.897 kb on - strand, within BPHYT_RS00075at 23.063 kb on + strand, within BPHYT_RS00075at 23.063 kb on + strand, within BPHYT_RS00075at 23.063 kb on + strand, within BPHYT_RS00075at 23.064 kb on - strand, within BPHYT_RS00075at 23.064 kb on - strand, within BPHYT_RS00075at 23.064 kb on - strand, within BPHYT_RS00075at 23.631 kb on - strand, within BPHYT_RS00075at 23.631 kb on - strand, within BPHYT_RS00075at 23.841 kb on - strand, within BPHYT_RS00075at 23.910 kb on + strand, within BPHYT_RS00075at 24.138 kb on + strand, within BPHYT_RS00075at 24.138 kb on + strand, within BPHYT_RS00075at 24.139 kb on - strand, within BPHYT_RS00075at 24.266 kb on - strandat 24.267 kb on + strandat 24.267 kb on + strandat 24.267 kb on + strandat 24.267 kb on + strandat 24.267 kb on + strandat 24.268 kb on - strandat 24.409 kb on + strandat 24.485 kb on - strandat 24.533 kb on - strandat 24.779 kb on + strand, within BPHYT_RS00080at 24.779 kb on + strand, within BPHYT_RS00080at 24.963 kb on + strand, within BPHYT_RS00080at 24.988 kb on + strand, within BPHYT_RS00080at 25.051 kb on + strand, within BPHYT_RS00080at 25.137 kb on + strandat 25.138 kb on - strandat 25.247 kb on - strandat 25.255 kb on + strandat 25.304 kb on + strand, within BPHYT_RS00085at 25.360 kb on + strand, within BPHYT_RS00085at 25.448 kb on + strand, within BPHYT_RS00085at 25.449 kb on - strand, within BPHYT_RS00085at 25.488 kb on + strand, within BPHYT_RS00085

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Per-strain Table

Position Strand Gene LocusTag Fraction 36hr PHYTO2 MOI 1
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20,355 - BPHYT_RS00070 0.35 -0.7
20,475 - BPHYT_RS00070 0.45 -0.8
20,478 + BPHYT_RS00070 0.45 +1.0
20,478 + BPHYT_RS00070 0.45 -0.5
20,478 + BPHYT_RS00070 0.45 -0.2
20,478 + BPHYT_RS00070 0.45 -0.3
20,594 - BPHYT_RS00070 0.55 +0.6
20,640 + BPHYT_RS00070 0.59 -0.7
20,640 + BPHYT_RS00070 0.59 +1.7
20,640 + BPHYT_RS00070 0.59 +1.6
20,641 - BPHYT_RS00070 0.59 +1.0
20,641 - BPHYT_RS00070 0.59 +0.4
20,642 + BPHYT_RS00070 0.59 -1.0
20,643 - BPHYT_RS00070 0.59 +0.1
20,643 - BPHYT_RS00070 0.59 -0.9
20,644 + BPHYT_RS00070 0.59 -0.6
20,644 + BPHYT_RS00070 0.59 -1.0
20,645 - BPHYT_RS00070 0.59 -0.6
20,645 - BPHYT_RS00070 0.59 -0.2
20,682 + BPHYT_RS00070 0.63 +0.5
20,682 + BPHYT_RS00070 0.63 -0.4
20,683 - BPHYT_RS00070 0.63 -0.3
20,683 - BPHYT_RS00070 0.63 +1.3
20,683 - BPHYT_RS00070 0.63 -2.2
20,683 - BPHYT_RS00070 0.63 -0.5
20,683 - BPHYT_RS00070 0.63 -0.4
20,683 - BPHYT_RS00070 0.63 +0.2
20,683 - BPHYT_RS00070 0.63 +0.2
20,876 + BPHYT_RS00070 0.79 -0.4
20,876 + BPHYT_RS00070 0.79 +0.1
21,041 + +1.2
21,042 - +0.4
21,222 - +0.1
21,240 + +0.1
21,240 + -0.5
21,241 - -0.1
21,241 - -0.8
21,378 - -0.6
21,430 + +0.3
21,430 + +0.9
21,430 + +1.1
21,431 - -0.5
21,431 - -0.4
21,431 - -1.0
21,433 - +0.1
21,480 + BPHYT_RS00075 0.11 +0.4
21,758 - BPHYT_RS00075 0.19 +0.4
21,759 + BPHYT_RS00075 0.19 -0.0
21,760 - BPHYT_RS00075 0.19 -0.5
21,774 + BPHYT_RS00075 0.19 +4.0
21,822 - BPHYT_RS00075 0.21 +0.6
21,898 + BPHYT_RS00075 0.23 -0.0
22,195 - BPHYT_RS00075 0.32 -2.1
22,195 - BPHYT_RS00075 0.32 -1.5
22,246 + BPHYT_RS00075 0.33 +0.8
22,247 - BPHYT_RS00075 0.33 +0.6
22,534 + BPHYT_RS00075 0.42 -0.2
22,612 + BPHYT_RS00075 0.44 -0.0
22,613 - BPHYT_RS00075 0.44 -1.6
22,632 + BPHYT_RS00075 0.45 +0.1
22,632 + BPHYT_RS00075 0.45 -1.5
22,800 + BPHYT_RS00075 0.50 -0.7
22,801 - BPHYT_RS00075 0.50 +1.0
22,801 - BPHYT_RS00075 0.50 +0.1
22,897 - BPHYT_RS00075 0.53 -0.0
23,063 + BPHYT_RS00075 0.58 +1.8
23,063 + BPHYT_RS00075 0.58 -0.7
23,063 + BPHYT_RS00075 0.58 -0.7
23,064 - BPHYT_RS00075 0.58 +1.3
23,064 - BPHYT_RS00075 0.58 +0.3
23,064 - BPHYT_RS00075 0.58 -2.0
23,631 - BPHYT_RS00075 0.74 +0.3
23,631 - BPHYT_RS00075 0.74 +0.0
23,841 - BPHYT_RS00075 0.81 +0.9
23,910 + BPHYT_RS00075 0.83 +0.6
24,138 + BPHYT_RS00075 0.89 +1.1
24,138 + BPHYT_RS00075 0.89 +0.7
24,139 - BPHYT_RS00075 0.89 +0.1
24,266 - -1.0
24,267 + +0.1
24,267 + +0.2
24,267 + -1.5
24,267 + +0.5
24,267 + +0.4
24,268 - +0.9
24,409 + -0.9
24,485 - +0.8
24,533 - +1.9
24,779 + BPHYT_RS00080 0.45 -2.1
24,779 + BPHYT_RS00080 0.45 +0.3
24,963 + BPHYT_RS00080 0.74 -0.7
24,988 + BPHYT_RS00080 0.77 -0.7
25,051 + BPHYT_RS00080 0.87 -2.3
25,137 + +1.2
25,138 - +1.4
25,247 - +1.7
25,255 + +0.2
25,304 + BPHYT_RS00085 0.12 -0.3
25,360 + BPHYT_RS00085 0.15 +0.5
25,448 + BPHYT_RS00085 0.21 +1.2
25,449 - BPHYT_RS00085 0.22 -1.6
25,488 + BPHYT_RS00085 0.24 +1.9

Or see this region's nucleotide sequence