Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS27980

Experiment: 36hr PHYTO1 MOI 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS27975 and BPHYT_RS27980 are separated by 67 nucleotidesBPHYT_RS27980 and BPHYT_RS27985 are separated by 114 nucleotides BPHYT_RS27975: BPHYT_RS27975 - ABC transporter permease, at 1,840,504 to 1,841,460 _RS27975 BPHYT_RS27980: BPHYT_RS27980 - ABC transporter substrate-binding protein, at 1,841,528 to 1,842,859 _RS27980 BPHYT_RS27985: BPHYT_RS27985 - LacI family transcriptional regulator, at 1,842,974 to 1,843,978 _RS27985 Position (kb) 1841 1842 1843Strain fitness (log2 ratio) -1 0 1at 1840.821 kb on - strand, within BPHYT_RS27975at 1841.783 kb on + strand, within BPHYT_RS27980at 1842.151 kb on - strand, within BPHYT_RS27980at 1842.257 kb on + strand, within BPHYT_RS27980at 1843.516 kb on - strand, within BPHYT_RS27985at 1843.516 kb on - strand, within BPHYT_RS27985at 1843.702 kb on - strand, within BPHYT_RS27985at 1843.824 kb on - strand, within BPHYT_RS27985at 1843.824 kb on - strand, within BPHYT_RS27985

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Per-strain Table

Position Strand Gene LocusTag Fraction 36hr PHYTO1 MOI 1
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1,840,821 - BPHYT_RS27975 0.33 +0.1
1,841,783 + BPHYT_RS27980 0.19 -0.5
1,842,151 - BPHYT_RS27980 0.47 -0.5
1,842,257 + BPHYT_RS27980 0.55 -1.0
1,843,516 - BPHYT_RS27985 0.54 +1.1
1,843,516 - BPHYT_RS27985 0.54 -1.7
1,843,702 - BPHYT_RS27985 0.72 -1.7
1,843,824 - BPHYT_RS27985 0.85 -1.0
1,843,824 - BPHYT_RS27985 0.85 +0.2

Or see this region's nucleotide sequence