Experiment: 36hr PHYTO1 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS22095 and BPHYT_RS22100 are separated by 418 nucleotides BPHYT_RS22100 and BPHYT_RS22105 are separated by 273 nucleotides
BPHYT_RS22095: BPHYT_RS22095 - porin, at 514,363 to 515,544
_RS22095
BPHYT_RS22100: BPHYT_RS22100 - ribonuclease BN, at 515,963 to 517,294
_RS22100
BPHYT_RS22105: BPHYT_RS22105 - tRNA synthetase, at 517,568 to 518,107
_RS22105
Position (kb) 515
516
517
518 Strain fitness (log2 ratio) -2
-1
0
1
2 at 515.012 kb on + strand, within BPHYT_RS22095 at 515.012 kb on + strand at 515.013 kb on - strand, within BPHYT_RS22095 at 515.013 kb on - strand, within BPHYT_RS22095 at 515.013 kb on - strand, within BPHYT_RS22095 at 515.054 kb on + strand, within BPHYT_RS22095 at 515.055 kb on - strand, within BPHYT_RS22095 at 515.200 kb on - strand, within BPHYT_RS22095 at 515.432 kb on + strand at 515.433 kb on - strand at 515.459 kb on + strand at 515.523 kb on + strand at 515.567 kb on - strand at 515.592 kb on + strand at 515.592 kb on + strand at 515.760 kb on + strand at 515.760 kb on + strand at 515.760 kb on + strand at 515.761 kb on - strand at 515.796 kb on + strand at 515.797 kb on - strand at 515.805 kb on + strand at 515.805 kb on + strand at 515.805 kb on + strand at 516.064 kb on - strand at 516.489 kb on + strand, within BPHYT_RS22100 at 516.489 kb on + strand, within BPHYT_RS22100 at 516.562 kb on - strand, within BPHYT_RS22100 at 516.608 kb on + strand, within BPHYT_RS22100 at 516.736 kb on - strand, within BPHYT_RS22100 at 516.916 kb on + strand, within BPHYT_RS22100 at 517.164 kb on + strand at 517.165 kb on - strand at 517.461 kb on - strand at 517.461 kb on - strand at 517.461 kb on - strand at 517.461 kb on - strand at 517.462 kb on + strand at 517.463 kb on - strand at 517.463 kb on - strand at 517.532 kb on + strand at 517.533 kb on - strand at 517.553 kb on + strand at 517.554 kb on - strand at 517.554 kb on - strand at 517.568 kb on - strand at 517.587 kb on + strand at 517.588 kb on - strand at 517.772 kb on + strand, within BPHYT_RS22105 at 518.052 kb on + strand, within BPHYT_RS22105 at 518.053 kb on - strand, within BPHYT_RS22105 at 518.053 kb on - strand, within BPHYT_RS22105 at 518.105 kb on + strand at 518.209 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO1 MOI 1 remove 515,012 + BPHYT_RS22095 0.55 -0.8 515,012 + +0.7 515,013 - BPHYT_RS22095 0.55 -0.6 515,013 - BPHYT_RS22095 0.55 +0.7 515,013 - BPHYT_RS22095 0.55 -1.0 515,054 + BPHYT_RS22095 0.58 +0.5 515,055 - BPHYT_RS22095 0.59 -0.8 515,200 - BPHYT_RS22095 0.71 -0.6 515,432 + -0.6 515,433 - +1.0 515,459 + +1.2 515,523 + -0.0 515,567 - -1.0 515,592 + +0.1 515,592 + -0.9 515,760 + -1.8 515,760 + +0.7 515,760 + -0.2 515,761 - -0.3 515,796 + +0.5 515,797 - -0.5 515,805 + +0.8 515,805 + -0.5 515,805 + +0.3 516,064 - +0.0 516,489 + BPHYT_RS22100 0.39 -0.3 516,489 + BPHYT_RS22100 0.39 -1.1 516,562 - BPHYT_RS22100 0.45 +1.3 516,608 + BPHYT_RS22100 0.48 -1.2 516,736 - BPHYT_RS22100 0.58 -1.3 516,916 + BPHYT_RS22100 0.72 -0.7 517,164 + +0.2 517,165 - -1.2 517,461 - +0.3 517,461 - -1.0 517,461 - +1.3 517,461 - +0.2 517,462 + -0.6 517,463 - +1.9 517,463 - -0.3 517,532 + -1.1 517,533 - +0.2 517,553 + -1.6 517,554 - -0.4 517,554 - +1.2 517,568 - -0.1 517,587 + +0.6 517,588 - +0.2 517,772 + BPHYT_RS22105 0.38 +0.1 518,052 + BPHYT_RS22105 0.90 -0.8 518,053 - BPHYT_RS22105 0.90 +0.5 518,053 - BPHYT_RS22105 0.90 +0.2 518,105 + -0.1 518,209 - +0.1
Or see this region's nucleotide sequence