Experiment: 36hr PHYTO1 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS21475 and BPHYT_RS21480 overlap by 25 nucleotides BPHYT_RS21480 and BPHYT_RS21485 are separated by 73 nucleotides BPHYT_RS21485 and BPHYT_RS21490 are separated by 175 nucleotides
BPHYT_RS21475: BPHYT_RS21475 - hypothetical protein, at 378,359 to 379,297
_RS21475
BPHYT_RS21480: BPHYT_RS21480 - hypothetical protein, at 379,273 to 379,863
_RS21480
BPHYT_RS21485: BPHYT_RS21485 - hypothetical protein, at 379,937 to 380,521
_RS21485
BPHYT_RS21490: BPHYT_RS21490 - ABC transporter permease, at 380,697 to 382,637
_RS21490
Position (kb) 379
380
381 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4 at 379.771 kb on + strand, within BPHYT_RS21480 at 379.772 kb on - strand, within BPHYT_RS21480 at 379.772 kb on - strand, within BPHYT_RS21480 at 379.774 kb on - strand, within BPHYT_RS21480 at 380.048 kb on - strand, within BPHYT_RS21485 at 380.575 kb on + strand at 380.576 kb on - strand at 380.576 kb on - strand at 380.580 kb on + strand at 380.580 kb on + strand at 380.580 kb on + strand at 380.581 kb on - strand at 380.827 kb on - strand at 380.842 kb on - strand at 380.851 kb on - strand at 380.877 kb on - strand at 380.970 kb on + strand, within BPHYT_RS21490 at 380.970 kb on + strand, within BPHYT_RS21490 at 380.970 kb on + strand, within BPHYT_RS21490 at 380.971 kb on - strand, within BPHYT_RS21490 at 381.129 kb on + strand, within BPHYT_RS21490 at 381.355 kb on + strand, within BPHYT_RS21490
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO1 MOI 1 remove 379,771 + BPHYT_RS21480 0.84 +0.9 379,772 - BPHYT_RS21480 0.84 +0.7 379,772 - BPHYT_RS21480 0.84 -0.5 379,774 - BPHYT_RS21480 0.85 +0.0 380,048 - BPHYT_RS21485 0.19 -2.1 380,575 + -0.2 380,576 - +0.2 380,576 - -0.6 380,580 + +0.3 380,580 + +0.9 380,580 + +0.7 380,581 - -0.3 380,827 - +1.3 380,842 - -0.5 380,851 - +4.6 380,877 - -1.4 380,970 + BPHYT_RS21490 0.14 +1.2 380,970 + BPHYT_RS21490 0.14 +0.5 380,970 + BPHYT_RS21490 0.14 -1.1 380,971 - BPHYT_RS21490 0.14 -0.1 381,129 + BPHYT_RS21490 0.22 +0.3 381,355 + BPHYT_RS21490 0.34 -0.2
Or see this region's nucleotide sequence