Experiment: 36hr PHYTO1 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS20010 and BPHYT_RS20015 are separated by 61 nucleotides BPHYT_RS20015 and BPHYT_RS20020 are separated by 331 nucleotides BPHYT_RS20020 and BPHYT_RS20025 are separated by 137 nucleotides
BPHYT_RS20010: BPHYT_RS20010 - membrane protein, at 33,343 to 34,482
_RS20010
BPHYT_RS20015: BPHYT_RS20015 - MFS transporter, at 34,544 to 35,791
_RS20015
BPHYT_RS20020: BPHYT_RS20020 - MarR family transcriptional regulator, at 36,123 to 36,623
_RS20020
BPHYT_RS20025: BPHYT_RS20025 - crotonase, at 36,761 to 37,588
_RS20025
Position (kb) 34
35
36 Strain fitness (log2 ratio) -2
-1
0
1 at 33.560 kb on + strand, within BPHYT_RS20010 at 33.561 kb on - strand, within BPHYT_RS20010 at 33.998 kb on + strand, within BPHYT_RS20010 at 34.046 kb on + strand, within BPHYT_RS20010 at 34.070 kb on + strand, within BPHYT_RS20010 at 34.112 kb on + strand, within BPHYT_RS20010 at 34.370 kb on + strand at 34.371 kb on - strand at 34.389 kb on + strand at 34.881 kb on + strand, within BPHYT_RS20015 at 34.881 kb on + strand, within BPHYT_RS20015 at 34.882 kb on - strand, within BPHYT_RS20015 at 35.046 kb on + strand, within BPHYT_RS20015 at 35.070 kb on + strand, within BPHYT_RS20015 at 35.325 kb on + strand, within BPHYT_RS20015 at 36.093 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO1 MOI 1 remove 33,560 + BPHYT_RS20010 0.19 +0.5 33,561 - BPHYT_RS20010 0.19 +0.4 33,998 + BPHYT_RS20010 0.57 -0.1 34,046 + BPHYT_RS20010 0.62 -0.2 34,070 + BPHYT_RS20010 0.64 -1.4 34,112 + BPHYT_RS20010 0.67 -0.4 34,370 + -0.4 34,371 - +0.4 34,389 + -2.0 34,881 + BPHYT_RS20015 0.27 +0.3 34,881 + BPHYT_RS20015 0.27 -0.9 34,882 - BPHYT_RS20015 0.27 -2.1 35,046 + BPHYT_RS20015 0.40 -2.1 35,070 + BPHYT_RS20015 0.42 -0.9 35,325 + BPHYT_RS20015 0.63 -2.6 36,093 + +0.2
Or see this region's nucleotide sequence