Experiment: 36hr PHYTO1 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS19990 and BPHYT_RS19995 are separated by 205 nucleotides BPHYT_RS19995 and BPHYT_RS20000 are separated by 6 nucleotides
BPHYT_RS19990: BPHYT_RS19990 - MFS transporter, at 27,774 to 29,207
_RS19990
BPHYT_RS19995: BPHYT_RS19995 - response regulator receiver, at 29,413 to 29,763
_RS19995
BPHYT_RS20000: BPHYT_RS20000 - serine/threonine protein kinase, at 29,770 to 31,245
_RS20000
Position (kb) 29
30 Strain fitness (log2 ratio) -2
-1
0
1
2 at 28.661 kb on - strand, within BPHYT_RS19990 at 28.726 kb on + strand, within BPHYT_RS19990 at 28.726 kb on + strand, within BPHYT_RS19990 at 28.726 kb on + strand, within BPHYT_RS19990 at 28.727 kb on - strand, within BPHYT_RS19990 at 28.727 kb on - strand, within BPHYT_RS19990 at 28.991 kb on - strand, within BPHYT_RS19990 at 29.023 kb on + strand, within BPHYT_RS19990 at 29.024 kb on - strand, within BPHYT_RS19990 at 29.044 kb on + strand, within BPHYT_RS19990 at 29.045 kb on - strand, within BPHYT_RS19990 at 29.288 kb on + strand at 29.289 kb on - strand at 29.323 kb on + strand at 29.324 kb on - strand at 29.324 kb on - strand at 29.327 kb on + strand at 29.328 kb on - strand at 29.415 kb on - strand at 29.475 kb on - strand, within BPHYT_RS19995 at 29.641 kb on + strand, within BPHYT_RS19995 at 29.641 kb on + strand, within BPHYT_RS19995 at 29.642 kb on - strand, within BPHYT_RS19995 at 29.686 kb on - strand, within BPHYT_RS19995 at 29.687 kb on + strand, within BPHYT_RS19995
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO1 MOI 1 remove 28,661 - BPHYT_RS19990 0.62 -1.2 28,726 + BPHYT_RS19990 0.66 +0.1 28,726 + BPHYT_RS19990 0.66 -0.5 28,726 + BPHYT_RS19990 0.66 +0.8 28,727 - BPHYT_RS19990 0.66 -0.5 28,727 - BPHYT_RS19990 0.66 -1.2 28,991 - BPHYT_RS19990 0.85 +1.3 29,023 + BPHYT_RS19990 0.87 -0.8 29,024 - BPHYT_RS19990 0.87 -0.3 29,044 + BPHYT_RS19990 0.89 -1.4 29,045 - BPHYT_RS19990 0.89 -1.1 29,288 + -0.7 29,289 - -0.4 29,323 + -0.2 29,324 - +1.8 29,324 - -0.2 29,327 + +0.5 29,328 - -0.0 29,415 - -0.5 29,475 - BPHYT_RS19995 0.18 -0.0 29,641 + BPHYT_RS19995 0.65 -2.0 29,641 + BPHYT_RS19995 0.65 +2.3 29,642 - BPHYT_RS19995 0.65 -0.1 29,686 - BPHYT_RS19995 0.78 -0.8 29,687 + BPHYT_RS19995 0.78 -0.1
Or see this region's nucleotide sequence