Experiment: 36hr PHYTO1 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS17345 and BPHYT_RS17350 are separated by 50 nucleotides BPHYT_RS17350 and BPHYT_RS17355 are separated by 2 nucleotides BPHYT_RS17355 and BPHYT_RS17360 are separated by 150 nucleotides
BPHYT_RS17345: BPHYT_RS17345 - bifunctional thiolase PaaJ: 3-oxo-5,6-didehydrosuberyl-CoA thiolase (EC 2.3.1.223); 3-oxoadipyl-CoA thiolase (EC 2.3.1.174) (from data) , at 3,932,532 to 3,933,734
_RS17345
BPHYT_RS17350: BPHYT_RS17350 - enoyl-CoA hydratase, at 3,933,785 to 3,934,576
_RS17350
BPHYT_RS17355: BPHYT_RS17355 - phenylacetic acid degradation protein, at 3,934,579 to 3,935,040
_RS17355
BPHYT_RS17360: BPHYT_RS17360 - phenylacetate-CoA ligase, at 3,935,191 to 3,936,495
_RS17360
Position (kb)
3934
3935
3936 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3933.735 kb on + strand at 3933.791 kb on + strand at 3933.791 kb on + strand at 3933.791 kb on + strand at 3933.791 kb on + strand at 3933.792 kb on - strand at 3933.846 kb on + strand at 3934.517 kb on + strand at 3934.652 kb on - strand, within BPHYT_RS17355 at 3934.700 kb on + strand, within BPHYT_RS17355 at 3934.825 kb on + strand, within BPHYT_RS17355 at 3934.825 kb on + strand, within BPHYT_RS17355 at 3934.825 kb on + strand, within BPHYT_RS17355 at 3934.825 kb on + strand, within BPHYT_RS17355 at 3934.826 kb on - strand, within BPHYT_RS17355 at 3934.826 kb on - strand, within BPHYT_RS17355 at 3934.826 kb on - strand, within BPHYT_RS17355 at 3934.826 kb on - strand, within BPHYT_RS17355 at 3934.826 kb on - strand, within BPHYT_RS17355 at 3934.892 kb on + strand, within BPHYT_RS17355 at 3934.893 kb on - strand, within BPHYT_RS17355 at 3934.942 kb on + strand, within BPHYT_RS17355 at 3934.942 kb on + strand, within BPHYT_RS17355 at 3934.943 kb on - strand, within BPHYT_RS17355 at 3934.943 kb on - strand, within BPHYT_RS17355 at 3935.151 kb on + strand at 3935.151 kb on + strand at 3935.151 kb on + strand at 3935.151 kb on + strand at 3935.152 kb on - strand at 3935.152 kb on - strand at 3935.152 kb on - strand at 3935.152 kb on - strand at 3935.152 kb on - strand at 3935.268 kb on + strand at 3935.269 kb on - strand at 3935.269 kb on - strand at 3935.291 kb on - strand at 3935.413 kb on + strand, within BPHYT_RS17360 at 3935.590 kb on - strand, within BPHYT_RS17360 at 3935.590 kb on - strand, within BPHYT_RS17360 at 3935.596 kb on + strand, within BPHYT_RS17360 at 3935.602 kb on + strand, within BPHYT_RS17360 at 3935.635 kb on + strand, within BPHYT_RS17360 at 3935.749 kb on + strand, within BPHYT_RS17360 at 3935.749 kb on + strand, within BPHYT_RS17360 at 3935.749 kb on + strand, within BPHYT_RS17360 at 3935.749 kb on + strand, within BPHYT_RS17360 at 3935.750 kb on - strand, within BPHYT_RS17360 at 3935.750 kb on - strand, within BPHYT_RS17360 at 3935.899 kb on + strand, within BPHYT_RS17360 at 3935.899 kb on + strand, within BPHYT_RS17360 at 3935.899 kb on + strand, within BPHYT_RS17360 at 3935.899 kb on + strand, within BPHYT_RS17360 at 3935.899 kb on + strand, within BPHYT_RS17360 at 3935.899 kb on + strand, within BPHYT_RS17360 at 3935.899 kb on + strand, within BPHYT_RS17360 at 3935.900 kb on - strand, within BPHYT_RS17360 at 3935.900 kb on - strand, within BPHYT_RS17360 at 3935.900 kb on - strand, within BPHYT_RS17360 at 3935.900 kb on - strand, within BPHYT_RS17360 at 3935.900 kb on - strand, within BPHYT_RS17360 at 3935.900 kb on - strand, within BPHYT_RS17360
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO1 MOI 1 remove 3,933,735 + +0.8 3,933,791 + +0.4 3,933,791 + +0.5 3,933,791 + -0.9 3,933,791 + -1.7 3,933,792 - -2.6 3,933,846 + -1.6 3,934,517 + -1.3 3,934,652 - BPHYT_RS17355 0.16 -0.3 3,934,700 + BPHYT_RS17355 0.26 -1.2 3,934,825 + BPHYT_RS17355 0.53 -1.1 3,934,825 + BPHYT_RS17355 0.53 +0.2 3,934,825 + BPHYT_RS17355 0.53 +0.4 3,934,825 + BPHYT_RS17355 0.53 +2.2 3,934,826 - BPHYT_RS17355 0.53 +0.8 3,934,826 - BPHYT_RS17355 0.53 -0.7 3,934,826 - BPHYT_RS17355 0.53 +0.1 3,934,826 - BPHYT_RS17355 0.53 -0.1 3,934,826 - BPHYT_RS17355 0.53 -0.1 3,934,892 + BPHYT_RS17355 0.68 -0.1 3,934,893 - BPHYT_RS17355 0.68 +0.3 3,934,942 + BPHYT_RS17355 0.79 -1.8 3,934,942 + BPHYT_RS17355 0.79 +1.4 3,934,943 - BPHYT_RS17355 0.79 +1.2 3,934,943 - BPHYT_RS17355 0.79 -0.0 3,935,151 + -0.4 3,935,151 + -1.6 3,935,151 + -0.2 3,935,151 + -0.2 3,935,152 - -0.4 3,935,152 - -0.9 3,935,152 - +0.1 3,935,152 - +0.4 3,935,152 - -0.0 3,935,268 + -0.4 3,935,269 - +2.4 3,935,269 - +0.2 3,935,291 - -0.1 3,935,413 + BPHYT_RS17360 0.17 -0.7 3,935,590 - BPHYT_RS17360 0.31 -1.2 3,935,590 - BPHYT_RS17360 0.31 -1.0 3,935,596 + BPHYT_RS17360 0.31 -0.6 3,935,602 + BPHYT_RS17360 0.31 -1.6 3,935,635 + BPHYT_RS17360 0.34 -1.5 3,935,749 + BPHYT_RS17360 0.43 -0.2 3,935,749 + BPHYT_RS17360 0.43 -1.2 3,935,749 + BPHYT_RS17360 0.43 +0.2 3,935,749 + BPHYT_RS17360 0.43 -0.2 3,935,750 - BPHYT_RS17360 0.43 -0.4 3,935,750 - BPHYT_RS17360 0.43 -0.1 3,935,899 + BPHYT_RS17360 0.54 +0.6 3,935,899 + BPHYT_RS17360 0.54 -0.2 3,935,899 + BPHYT_RS17360 0.54 -0.3 3,935,899 + BPHYT_RS17360 0.54 -1.1 3,935,899 + BPHYT_RS17360 0.54 +0.4 3,935,899 + BPHYT_RS17360 0.54 -0.1 3,935,899 + BPHYT_RS17360 0.54 -0.5 3,935,900 - BPHYT_RS17360 0.54 +0.3 3,935,900 - BPHYT_RS17360 0.54 +0.7 3,935,900 - BPHYT_RS17360 0.54 -0.2 3,935,900 - BPHYT_RS17360 0.54 +0.3 3,935,900 - BPHYT_RS17360 0.54 +0.6 3,935,900 - BPHYT_RS17360 0.54 +1.2
Or see this region's nucleotide sequence