Experiment: 36hr PHYTO1 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS16115 and BPHYT_RS16120 are separated by 72 nucleotides BPHYT_RS16120 and BPHYT_RS16125 are separated by 332 nucleotides BPHYT_RS16125 and BPHYT_RS16130 are separated by 59 nucleotides
BPHYT_RS16115: BPHYT_RS16115 - ABC transporter for D-Sorbitol, periplasmic substrate-binding component (from data) , at 3,655,894 to 3,657,216
_RS16115
BPHYT_RS16120: BPHYT_RS16120 - sorbitol dehydrogenase, D-fructose forming (EC 1.1.1.14) (from data) , at 3,657,289 to 3,658,071
_RS16120
BPHYT_RS16125: BPHYT_RS16125 - Fur family transcriptional regulator, at 3,658,404 to 3,658,898
_RS16125
BPHYT_RS16130: BPHYT_RS16130 - cation ABC transporter substrate-binding protein, at 3,658,958 to 3,659,926
_RS16130
Position (kb)
3657
3658
3659 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 3656.400 kb on - strand, within BPHYT_RS16115 at 3656.515 kb on + strand, within BPHYT_RS16115 at 3656.528 kb on + strand, within BPHYT_RS16115 at 3656.529 kb on - strand, within BPHYT_RS16115 at 3656.529 kb on - strand, within BPHYT_RS16115 at 3656.627 kb on + strand at 3656.675 kb on + strand, within BPHYT_RS16115 at 3656.730 kb on - strand, within BPHYT_RS16115 at 3656.786 kb on + strand, within BPHYT_RS16115 at 3656.786 kb on + strand, within BPHYT_RS16115 at 3656.786 kb on + strand, within BPHYT_RS16115 at 3656.787 kb on - strand, within BPHYT_RS16115 at 3656.807 kb on + strand, within BPHYT_RS16115 at 3656.808 kb on - strand, within BPHYT_RS16115 at 3656.808 kb on - strand, within BPHYT_RS16115 at 3656.822 kb on + strand, within BPHYT_RS16115 at 3656.822 kb on + strand, within BPHYT_RS16115 at 3657.240 kb on + strand at 3657.244 kb on + strand at 3657.244 kb on + strand at 3657.244 kb on + strand at 3657.461 kb on + strand, within BPHYT_RS16120 at 3657.461 kb on + strand, within BPHYT_RS16120 at 3657.501 kb on + strand, within BPHYT_RS16120 at 3657.576 kb on - strand, within BPHYT_RS16120 at 3657.651 kb on + strand, within BPHYT_RS16120 at 3657.651 kb on + strand, within BPHYT_RS16120 at 3657.652 kb on - strand, within BPHYT_RS16120 at 3657.915 kb on - strand, within BPHYT_RS16120 at 3657.915 kb on - strand, within BPHYT_RS16120 at 3657.993 kb on - strand at 3658.253 kb on + strand at 3658.253 kb on + strand at 3658.254 kb on - strand at 3658.254 kb on - strand at 3658.254 kb on - strand at 3658.254 kb on - strand at 3658.257 kb on - strand at 3658.322 kb on - strand at 3658.507 kb on - strand, within BPHYT_RS16125 at 3658.507 kb on - strand, within BPHYT_RS16125 at 3658.545 kb on + strand, within BPHYT_RS16125 at 3658.546 kb on - strand, within BPHYT_RS16125 at 3658.600 kb on + strand, within BPHYT_RS16125 at 3658.600 kb on + strand, within BPHYT_RS16125 at 3658.600 kb on + strand, within BPHYT_RS16125 at 3658.600 kb on + strand, within BPHYT_RS16125 at 3658.600 kb on + strand, within BPHYT_RS16125 at 3658.914 kb on + strand at 3658.914 kb on + strand at 3658.914 kb on + strand at 3658.914 kb on + strand at 3658.915 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO1 MOI 1 remove 3,656,400 - BPHYT_RS16115 0.38 +1.1 3,656,515 + BPHYT_RS16115 0.47 -0.1 3,656,528 + BPHYT_RS16115 0.48 +0.5 3,656,529 - BPHYT_RS16115 0.48 -0.4 3,656,529 - BPHYT_RS16115 0.48 +0.2 3,656,627 + -1.1 3,656,675 + BPHYT_RS16115 0.59 -0.1 3,656,730 - BPHYT_RS16115 0.63 -1.2 3,656,786 + BPHYT_RS16115 0.67 -0.8 3,656,786 + BPHYT_RS16115 0.67 -1.5 3,656,786 + BPHYT_RS16115 0.67 +0.5 3,656,787 - BPHYT_RS16115 0.67 +2.2 3,656,807 + BPHYT_RS16115 0.69 -0.4 3,656,808 - BPHYT_RS16115 0.69 -0.9 3,656,808 - BPHYT_RS16115 0.69 +0.5 3,656,822 + BPHYT_RS16115 0.70 +0.3 3,656,822 + BPHYT_RS16115 0.70 +1.2 3,657,240 + +0.5 3,657,244 + +0.2 3,657,244 + +0.2 3,657,244 + -0.1 3,657,461 + BPHYT_RS16120 0.22 +0.3 3,657,461 + BPHYT_RS16120 0.22 -0.3 3,657,501 + BPHYT_RS16120 0.27 +2.6 3,657,576 - BPHYT_RS16120 0.37 -0.6 3,657,651 + BPHYT_RS16120 0.46 -0.9 3,657,651 + BPHYT_RS16120 0.46 +3.8 3,657,652 - BPHYT_RS16120 0.46 +2.2 3,657,915 - BPHYT_RS16120 0.80 +0.6 3,657,915 - BPHYT_RS16120 0.80 +0.1 3,657,993 - +0.9 3,658,253 + +0.8 3,658,253 + +0.2 3,658,254 - -1.8 3,658,254 - -0.7 3,658,254 - +1.1 3,658,254 - -0.9 3,658,257 - +0.1 3,658,322 - +1.7 3,658,507 - BPHYT_RS16125 0.21 +0.2 3,658,507 - BPHYT_RS16125 0.21 +1.0 3,658,545 + BPHYT_RS16125 0.28 -0.1 3,658,546 - BPHYT_RS16125 0.29 +0.2 3,658,600 + BPHYT_RS16125 0.40 -0.8 3,658,600 + BPHYT_RS16125 0.40 +1.0 3,658,600 + BPHYT_RS16125 0.40 -1.1 3,658,600 + BPHYT_RS16125 0.40 -0.5 3,658,600 + BPHYT_RS16125 0.40 -0.1 3,658,914 + +2.2 3,658,914 + -0.1 3,658,914 + -0.0 3,658,914 + -1.6 3,658,915 - -1.4
Or see this region's nucleotide sequence