Experiment: 36hr PHYTO1 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS12625 and BPHYT_RS12630 are separated by 6 nucleotides BPHYT_RS12630 and BPHYT_RS12635 are separated by 47 nucleotides BPHYT_RS12635 and BPHYT_RS12640 are separated by 130 nucleotides
BPHYT_RS12625: BPHYT_RS12625 - tryptophanyl-tRNA synthetase, at 2,880,871 to 2,882,073
_RS12625
BPHYT_RS12630: BPHYT_RS12630 - peptidase M50, at 2,882,080 to 2,882,742
_RS12630
BPHYT_RS12635: BPHYT_RS12635 - translation factor Sua5, at 2,882,790 to 2,883,425
_RS12635
BPHYT_RS12640: BPHYT_RS12640 - phosphoesterase, at 2,883,556 to 2,884,386
_RS12640
Position (kb)
2882
2883
2884 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 2881.922 kb on + strand, within BPHYT_RS12625 at 2882.058 kb on + strand at 2882.058 kb on + strand at 2882.059 kb on - strand at 2882.077 kb on + strand at 2882.078 kb on - strand at 2882.756 kb on - strand at 2883.137 kb on - strand, within BPHYT_RS12635 at 2883.137 kb on - strand, within BPHYT_RS12635 at 2883.313 kb on + strand, within BPHYT_RS12635 at 2883.314 kb on - strand, within BPHYT_RS12635 at 2883.416 kb on - strand at 2883.416 kb on - strand at 2883.416 kb on - strand at 2883.460 kb on + strand at 2883.460 kb on + strand at 2883.461 kb on - strand at 2883.501 kb on - strand at 2883.695 kb on + strand, within BPHYT_RS12640 at 2883.801 kb on - strand, within BPHYT_RS12640 at 2883.892 kb on + strand, within BPHYT_RS12640 at 2883.893 kb on - strand, within BPHYT_RS12640 at 2883.893 kb on - strand, within BPHYT_RS12640 at 2883.897 kb on - strand, within BPHYT_RS12640 at 2884.013 kb on - strand, within BPHYT_RS12640 at 2884.013 kb on - strand, within BPHYT_RS12640 at 2884.016 kb on + strand, within BPHYT_RS12640 at 2884.017 kb on - strand, within BPHYT_RS12640 at 2884.017 kb on - strand, within BPHYT_RS12640 at 2884.017 kb on - strand, within BPHYT_RS12640 at 2884.017 kb on - strand, within BPHYT_RS12640 at 2884.017 kb on - strand, within BPHYT_RS12640 at 2884.017 kb on - strand, within BPHYT_RS12640 at 2884.017 kb on - strand, within BPHYT_RS12640 at 2884.017 kb on - strand, within BPHYT_RS12640 at 2884.017 kb on - strand, within BPHYT_RS12640 at 2884.164 kb on + strand, within BPHYT_RS12640 at 2884.164 kb on + strand, within BPHYT_RS12640 at 2884.215 kb on - strand, within BPHYT_RS12640 at 2884.215 kb on - strand, within BPHYT_RS12640 at 2884.418 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO1 MOI 1 remove 2,881,922 + BPHYT_RS12625 0.87 +0.9 2,882,058 + +0.4 2,882,058 + -1.2 2,882,059 - -0.4 2,882,077 + +0.6 2,882,078 - -0.2 2,882,756 - +1.4 2,883,137 - BPHYT_RS12635 0.55 +0.8 2,883,137 - BPHYT_RS12635 0.55 +2.0 2,883,313 + BPHYT_RS12635 0.82 +3.2 2,883,314 - BPHYT_RS12635 0.82 +0.6 2,883,416 - +0.3 2,883,416 - +0.7 2,883,416 - -0.5 2,883,460 + -0.6 2,883,460 + -1.5 2,883,461 - +1.3 2,883,501 - +0.1 2,883,695 + BPHYT_RS12640 0.17 -2.2 2,883,801 - BPHYT_RS12640 0.29 +0.5 2,883,892 + BPHYT_RS12640 0.40 -0.4 2,883,893 - BPHYT_RS12640 0.41 +0.9 2,883,893 - BPHYT_RS12640 0.41 -1.4 2,883,897 - BPHYT_RS12640 0.41 -0.2 2,884,013 - BPHYT_RS12640 0.55 -0.5 2,884,013 - BPHYT_RS12640 0.55 +0.3 2,884,016 + BPHYT_RS12640 0.55 +3.8 2,884,017 - BPHYT_RS12640 0.55 -0.1 2,884,017 - BPHYT_RS12640 0.55 +0.3 2,884,017 - BPHYT_RS12640 0.55 +0.2 2,884,017 - BPHYT_RS12640 0.55 +0.2 2,884,017 - BPHYT_RS12640 0.55 +1.1 2,884,017 - BPHYT_RS12640 0.55 +0.2 2,884,017 - BPHYT_RS12640 0.55 +1.1 2,884,017 - BPHYT_RS12640 0.55 -0.1 2,884,017 - BPHYT_RS12640 0.55 +1.0 2,884,164 + BPHYT_RS12640 0.73 +3.2 2,884,164 + BPHYT_RS12640 0.73 +1.2 2,884,215 - BPHYT_RS12640 0.79 -0.1 2,884,215 - BPHYT_RS12640 0.79 +1.7 2,884,418 - +0.5
Or see this region's nucleotide sequence