Experiment: 36hr PHYTO1 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS11790 and BPHYT_RS11795 are separated by 53 nucleotides BPHYT_RS11795 and BPHYT_RS11800 overlap by 8 nucleotides BPHYT_RS11800 and BPHYT_RS11805 are separated by 45 nucleotides BPHYT_RS11805 and BPHYT_RS11810 are separated by 351 nucleotides
BPHYT_RS11790: BPHYT_RS11790 - fumarylacetoacetase, at 2,673,401 to 2,674,723
_RS11790
BPHYT_RS11795: BPHYT_RS11795 - membrane protein, at 2,674,777 to 2,675,151
_RS11795
BPHYT_RS11800: BPHYT_RS11800 - acetate permease, at 2,675,144 to 2,676,730
_RS11800
BPHYT_RS11805: BPHYT_RS11805 - hypothetical protein, at 2,676,776 to 2,677,369
_RS11805
BPHYT_RS11810: BPHYT_RS11810 - LysR family transcriptional regulator, at 2,677,721 to 2,678,599
_RS11810
Position (kb)
2675
2676
2677 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2674.302 kb on - strand, within BPHYT_RS11790 at 2675.047 kb on - strand, within BPHYT_RS11795 at 2675.463 kb on - strand, within BPHYT_RS11800 at 2675.463 kb on - strand, within BPHYT_RS11800 at 2675.654 kb on + strand, within BPHYT_RS11800 at 2675.688 kb on - strand, within BPHYT_RS11800 at 2675.860 kb on + strand, within BPHYT_RS11800 at 2676.000 kb on + strand, within BPHYT_RS11800 at 2676.067 kb on + strand, within BPHYT_RS11800 at 2676.068 kb on - strand, within BPHYT_RS11800 at 2676.224 kb on - strand, within BPHYT_RS11800 at 2676.693 kb on + strand at 2676.781 kb on + strand at 2676.818 kb on - strand at 2677.080 kb on - strand, within BPHYT_RS11805 at 2677.226 kb on + strand, within BPHYT_RS11805 at 2677.311 kb on + strand at 2677.721 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO1 MOI 1 remove 2,674,302 - BPHYT_RS11790 0.68 -0.0 2,675,047 - BPHYT_RS11795 0.72 -0.2 2,675,463 - BPHYT_RS11800 0.20 -0.3 2,675,463 - BPHYT_RS11800 0.20 +0.3 2,675,654 + BPHYT_RS11800 0.32 +0.4 2,675,688 - BPHYT_RS11800 0.34 +0.8 2,675,860 + BPHYT_RS11800 0.45 +0.3 2,676,000 + BPHYT_RS11800 0.54 -0.3 2,676,067 + BPHYT_RS11800 0.58 -1.1 2,676,068 - BPHYT_RS11800 0.58 +0.3 2,676,224 - BPHYT_RS11800 0.68 -1.3 2,676,693 + -0.4 2,676,781 + +1.4 2,676,818 - +0.6 2,677,080 - BPHYT_RS11805 0.51 +0.1 2,677,226 + BPHYT_RS11805 0.76 -2.9 2,677,311 + -1.3 2,677,721 - -0.8
Or see this region's nucleotide sequence