Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS10640

Experiment: 36hr PHYTO1 MOI 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS10635 and BPHYT_RS10640 are separated by 123 nucleotidesBPHYT_RS10640 and BPHYT_RS10645 are separated by 39 nucleotidesBPHYT_RS10645 and BPHYT_RS10650 are separated by 197 nucleotides BPHYT_RS10635: BPHYT_RS10635 - transcriptional regulator, at 2,423,710 to 2,424,624 _RS10635 BPHYT_RS10640: BPHYT_RS10640 - carbonic anhydrase, at 2,424,748 to 2,425,407 _RS10640 BPHYT_RS10645: BPHYT_RS10645 - cyanate hydratase, at 2,425,447 to 2,425,917 _RS10645 BPHYT_RS10650: BPHYT_RS10650 - membrane protein, at 2,426,115 to 2,426,846 _RS10650 Position (kb) 2424 2425 2426Strain fitness (log2 ratio) -1 0 1at 2423.832 kb on + strand, within BPHYT_RS10635at 2424.435 kb on - strand, within BPHYT_RS10635at 2425.120 kb on + strand, within BPHYT_RS10640at 2425.429 kb on - strandat 2425.756 kb on + strand, within BPHYT_RS10645at 2426.055 kb on + strandat 2426.166 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 36hr PHYTO1 MOI 1
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2,423,832 + BPHYT_RS10635 0.13 -0.5
2,424,435 - BPHYT_RS10635 0.79 +0.5
2,425,120 + BPHYT_RS10640 0.56 +0.9
2,425,429 - +0.1
2,425,756 + BPHYT_RS10645 0.66 +1.5
2,426,055 + +1.2
2,426,166 - +0.4

Or see this region's nucleotide sequence