Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS04285

Experiment: 36hr PHYTO1 MOI 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS04275 and BPHYT_RS04280 are separated by 9 nucleotidesBPHYT_RS04280 and BPHYT_RS04285 overlap by 19 nucleotidesBPHYT_RS04285 and BPHYT_RS04290 are separated by 327 nucleotides BPHYT_RS04275: BPHYT_RS04275 - dTDP-4-dehydrorhamnose 3,5-epimerase, at 979,311 to 979,862 _RS04275 BPHYT_RS04280: BPHYT_RS04280 - dTDP-4-dehydrorhamnose reductase, at 979,872 to 980,768 _RS04280 BPHYT_RS04285: BPHYT_RS04285 - rhamnosyltransferase, at 980,750 to 981,661 _RS04285 BPHYT_RS04290: BPHYT_RS04290 - glycosyl transferase family 2, at 981,989 to 983,914 _RS04290 Position (kb) 980 981 982Strain fitness (log2 ratio) -1 0 1 2 3at 981.138 kb on - strand, within BPHYT_RS04285at 981.468 kb on - strand, within BPHYT_RS04285at 981.468 kb on - strand, within BPHYT_RS04285at 981.652 kb on + strandat 981.659 kb on + strandat 982.091 kb on + strandat 982.196 kb on + strand, within BPHYT_RS04290at 982.587 kb on - strand, within BPHYT_RS04290

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Per-strain Table

Position Strand Gene LocusTag Fraction 36hr PHYTO1 MOI 1
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981,138 - BPHYT_RS04285 0.43 +3.3
981,468 - BPHYT_RS04285 0.79 +2.3
981,468 - BPHYT_RS04285 0.79 +1.3
981,652 + -1.1
981,659 + -0.6
982,091 + +1.3
982,196 + BPHYT_RS04290 0.11 +1.3
982,587 - BPHYT_RS04290 0.31 +2.3

Or see this region's nucleotide sequence