Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS00355

Experiment: 36hr PHYTO1 MOI 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS00355 and BPHYT_RS00360 are separated by 317 nucleotides BPHYT_RS00355: BPHYT_RS00355 - porin, at 89,804 to 90,931 _RS00355 BPHYT_RS00360: BPHYT_RS00360 - protoporphyrinogen oxidase, at 91,249 to 92,604 _RS00360 Position (kb) 89 90 91Strain fitness (log2 ratio) -2 -1 0 1 2at 88.973 kb on - strandat 89.116 kb on + strandat 89.187 kb on - strandat 89.190 kb on + strandat 89.191 kb on - strandat 89.322 kb on - strandat 89.341 kb on - strandat 89.419 kb on - strandat 89.422 kb on + strandat 89.423 kb on - strandat 89.423 kb on - strandat 89.700 kb on - strandat 89.830 kb on - strandat 89.830 kb on - strandat 90.129 kb on - strand, within BPHYT_RS00355at 90.457 kb on + strand, within BPHYT_RS00355at 90.457 kb on + strand, within BPHYT_RS00355at 90.458 kb on - strand, within BPHYT_RS00355at 90.458 kb on - strand, within BPHYT_RS00355at 90.512 kb on - strand, within BPHYT_RS00355at 90.516 kb on - strand, within BPHYT_RS00355at 90.516 kb on - strand, within BPHYT_RS00355at 90.696 kb on - strand, within BPHYT_RS00355at 90.797 kb on + strandat 90.840 kb on - strandat 90.923 kb on - strandat 91.319 kb on + strandat 91.320 kb on - strandat 91.320 kb on - strandat 91.545 kb on - strand, within BPHYT_RS00360at 91.574 kb on + strand, within BPHYT_RS00360at 91.739 kb on - strand, within BPHYT_RS00360at 91.854 kb on + strand, within BPHYT_RS00360at 91.874 kb on + strand, within BPHYT_RS00360at 91.874 kb on + strand, within BPHYT_RS00360at 91.875 kb on - strand, within BPHYT_RS00360

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Per-strain Table

Position Strand Gene LocusTag Fraction 36hr PHYTO1 MOI 1
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88,973 - -0.6
89,116 + -1.3
89,187 - -0.2
89,190 + -1.5
89,191 - +0.1
89,322 - -0.2
89,341 - -0.4
89,419 - +0.4
89,422 + +1.3
89,423 - -0.5
89,423 - +0.9
89,700 - -0.0
89,830 - -1.6
89,830 - -0.6
90,129 - BPHYT_RS00355 0.29 -1.1
90,457 + BPHYT_RS00355 0.58 -0.2
90,457 + BPHYT_RS00355 0.58 +0.0
90,458 - BPHYT_RS00355 0.58 +0.4
90,458 - BPHYT_RS00355 0.58 -0.9
90,512 - BPHYT_RS00355 0.63 +0.2
90,516 - BPHYT_RS00355 0.63 -0.4
90,516 - BPHYT_RS00355 0.63 -2.5
90,696 - BPHYT_RS00355 0.79 -0.8
90,797 + +2.3
90,840 - +2.6
90,923 - +0.0
91,319 + -0.3
91,320 - -0.6
91,320 - -0.3
91,545 - BPHYT_RS00360 0.22 -0.2
91,574 + BPHYT_RS00360 0.24 -0.2
91,739 - BPHYT_RS00360 0.36 -0.3
91,854 + BPHYT_RS00360 0.45 -0.6
91,874 + BPHYT_RS00360 0.46 +0.5
91,874 + BPHYT_RS00360 0.46 +0.0
91,875 - BPHYT_RS00360 0.46 +2.3

Or see this region's nucleotide sequence