Experiment: 36hr PHYTO1 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS34015 and BPHYT_RS34020 overlap by 1 nucleotides BPHYT_RS34020 and BPHYT_RS34025 overlap by 4 nucleotides BPHYT_RS34025 and BPHYT_RS34030 are separated by 234 nucleotides
BPHYT_RS34015: BPHYT_RS34015 - hypothetical protein, at 3,230,709 to 3,232,235
_RS34015
BPHYT_RS34020: BPHYT_RS34020 - hypothetical protein, at 3,232,235 to 3,233,119
_RS34020
BPHYT_RS34025: BPHYT_RS34025 - hypothetical protein, at 3,233,116 to 3,233,835
_RS34025
BPHYT_RS34030: BPHYT_RS34030 - pilus assembly protein CpaB, at 3,234,070 to 3,235,170
_RS34030
Position (kb)
3233
3234 Strain fitness (log2 ratio)
-2
-1
0
1 at 3232.249 kb on + strand at 3232.249 kb on + strand at 3232.484 kb on + strand, within BPHYT_RS34020 at 3232.485 kb on - strand, within BPHYT_RS34020 at 3232.507 kb on - strand, within BPHYT_RS34020 at 3232.507 kb on - strand, within BPHYT_RS34020 at 3232.721 kb on - strand, within BPHYT_RS34020 at 3232.721 kb on - strand, within BPHYT_RS34020 at 3232.934 kb on - strand, within BPHYT_RS34020 at 3233.025 kb on - strand, within BPHYT_RS34020 at 3233.091 kb on + strand at 3233.702 kb on - strand, within BPHYT_RS34025 at 3233.982 kb on - strand at 3234.116 kb on + strand at 3234.137 kb on + strand at 3234.567 kb on - strand, within BPHYT_RS34030 at 3234.569 kb on - strand, within BPHYT_RS34030 at 3234.740 kb on - strand, within BPHYT_RS34030
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO1 MOI 1 remove 3,232,249 + +0.3 3,232,249 + +0.4 3,232,484 + BPHYT_RS34020 0.28 -1.0 3,232,485 - BPHYT_RS34020 0.28 +1.2 3,232,507 - BPHYT_RS34020 0.31 +0.0 3,232,507 - BPHYT_RS34020 0.31 +0.1 3,232,721 - BPHYT_RS34020 0.55 -1.6 3,232,721 - BPHYT_RS34020 0.55 -0.4 3,232,934 - BPHYT_RS34020 0.79 -2.2 3,233,025 - BPHYT_RS34020 0.89 -0.4 3,233,091 + -0.3 3,233,702 - BPHYT_RS34025 0.81 +0.7 3,233,982 - -0.4 3,234,116 + -0.5 3,234,137 + -0.8 3,234,567 - BPHYT_RS34030 0.45 +0.4 3,234,569 - BPHYT_RS34030 0.45 +1.0 3,234,740 - BPHYT_RS34030 0.61 -0.2
Or see this region's nucleotide sequence