Experiment: 36hr PHYTO1 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS33800 and BPHYT_RS33805 are separated by 181 nucleotides BPHYT_RS33805 and BPHYT_RS33810 overlap by 19 nucleotides BPHYT_RS33810 and BPHYT_RS33815 are separated by 59 nucleotides
BPHYT_RS33800: BPHYT_RS33800 - glycosyl hydrolase, at 3,174,620 to 3,177,475
_RS33800
BPHYT_RS33805: BPHYT_RS33805 - hypothetical protein, at 3,177,657 to 3,177,866
_RS33805
BPHYT_RS33810: BPHYT_RS33810 - membrane protein, at 3,177,848 to 3,178,570
_RS33810
BPHYT_RS33815: BPHYT_RS33815 - hypothetical protein, at 3,178,630 to 3,179,973
_RS33815
Position (kb)
3177
3178
3179 Strain fitness (log2 ratio)
-2
-1
0
1 at 3177.153 kb on - strand, within BPHYT_RS33800 at 3177.407 kb on + strand at 3177.510 kb on + strand at 3177.788 kb on - strand, within BPHYT_RS33805 at 3178.114 kb on - strand, within BPHYT_RS33810 at 3178.114 kb on - strand, within BPHYT_RS33810 at 3178.356 kb on + strand, within BPHYT_RS33810 at 3178.599 kb on - strand at 3179.066 kb on + strand, within BPHYT_RS33815 at 3179.067 kb on - strand, within BPHYT_RS33815 at 3179.067 kb on - strand, within BPHYT_RS33815 at 3179.067 kb on - strand, within BPHYT_RS33815 at 3179.432 kb on + strand, within BPHYT_RS33815 at 3179.520 kb on - strand, within BPHYT_RS33815 at 3179.541 kb on - strand, within BPHYT_RS33815
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO1 MOI 1 remove 3,177,153 - BPHYT_RS33800 0.89 -0.4 3,177,407 + +0.1 3,177,510 + +0.2 3,177,788 - BPHYT_RS33805 0.62 -2.5 3,178,114 - BPHYT_RS33810 0.37 -0.8 3,178,114 - BPHYT_RS33810 0.37 -0.6 3,178,356 + BPHYT_RS33810 0.70 +0.9 3,178,599 - -0.8 3,179,066 + BPHYT_RS33815 0.32 +0.3 3,179,067 - BPHYT_RS33815 0.33 -1.0 3,179,067 - BPHYT_RS33815 0.33 -0.1 3,179,067 - BPHYT_RS33815 0.33 -0.8 3,179,432 + BPHYT_RS33815 0.60 -1.6 3,179,520 - BPHYT_RS33815 0.66 +0.7 3,179,541 - BPHYT_RS33815 0.68 +1.1
Or see this region's nucleotide sequence