Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS33385

Experiment: 36hr PHYTO1 MOI 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS33380 and BPHYT_RS33385 are separated by 50 nucleotidesBPHYT_RS33385 and BPHYT_RS33390 are separated by 18 nucleotides BPHYT_RS33380: BPHYT_RS33380 - 2-nitropropane dioxygenase, at 3,072,246 to 3,073,295 _RS33380 BPHYT_RS33385: BPHYT_RS33385 - major facilitator superfamily protein, at 3,073,346 to 3,074,617 _RS33385 BPHYT_RS33390: BPHYT_RS33390 - phosphate ABC transporter substrate-binding protein, at 3,074,636 to 3,075,670 _RS33390 Position (kb) 3073 3074 3075Strain fitness (log2 ratio) -1 0 1 2 3at 3072.576 kb on + strand, within BPHYT_RS33380at 3073.102 kb on - strand, within BPHYT_RS33380at 3073.111 kb on + strand, within BPHYT_RS33380at 3073.111 kb on + strand, within BPHYT_RS33380at 3073.144 kb on - strand, within BPHYT_RS33380at 3073.155 kb on + strand, within BPHYT_RS33380at 3073.403 kb on + strandat 3073.403 kb on + strandat 3073.511 kb on + strand, within BPHYT_RS33385at 3074.451 kb on - strand, within BPHYT_RS33385at 3074.728 kb on + strandat 3074.838 kb on + strand, within BPHYT_RS33390at 3074.839 kb on - strand, within BPHYT_RS33390at 3074.998 kb on + strand, within BPHYT_RS33390at 3075.056 kb on + strand, within BPHYT_RS33390at 3075.056 kb on + strand, within BPHYT_RS33390at 3075.093 kb on - strand, within BPHYT_RS33390at 3075.297 kb on + strand, within BPHYT_RS33390at 3075.298 kb on - strand, within BPHYT_RS33390at 3075.298 kb on - strand, within BPHYT_RS33390at 3075.346 kb on + strand, within BPHYT_RS33390at 3075.599 kb on - strandat 3075.600 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 36hr PHYTO1 MOI 1
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3,072,576 + BPHYT_RS33380 0.31 +0.2
3,073,102 - BPHYT_RS33380 0.82 -0.9
3,073,111 + BPHYT_RS33380 0.82 -0.5
3,073,111 + BPHYT_RS33380 0.82 +0.3
3,073,144 - BPHYT_RS33380 0.86 -0.0
3,073,155 + BPHYT_RS33380 0.87 +1.2
3,073,403 + +0.1
3,073,403 + +0.2
3,073,511 + BPHYT_RS33385 0.13 -1.2
3,074,451 - BPHYT_RS33385 0.87 -0.3
3,074,728 + -0.5
3,074,838 + BPHYT_RS33390 0.20 +0.5
3,074,839 - BPHYT_RS33390 0.20 +0.5
3,074,998 + BPHYT_RS33390 0.35 +2.3
3,075,056 + BPHYT_RS33390 0.41 +0.5
3,075,056 + BPHYT_RS33390 0.41 +1.9
3,075,093 - BPHYT_RS33390 0.44 -0.4
3,075,297 + BPHYT_RS33390 0.64 -0.6
3,075,298 - BPHYT_RS33390 0.64 +3.7
3,075,298 - BPHYT_RS33390 0.64 -0.1
3,075,346 + BPHYT_RS33390 0.69 -1.4
3,075,599 - +0.4
3,075,600 + +1.3

Or see this region's nucleotide sequence