Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS31305

Experiment: 36hr PHYTO1 MOI 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS31300 and BPHYT_RS31305 are separated by 81 nucleotidesBPHYT_RS31305 and BPHYT_RS31310 are separated by 94 nucleotidesBPHYT_RS31310 and BPHYT_RS31315 are separated by 245 nucleotides BPHYT_RS31300: BPHYT_RS31300 - Crp/Fnr family transcriptional regulator, at 2,581,378 to 2,582,166 _RS31300 BPHYT_RS31305: BPHYT_RS31305 - alcohol dehydrogenase, at 2,582,248 to 2,583,234 _RS31305 BPHYT_RS31310: BPHYT_RS31310 - hypothetical protein, at 2,583,329 to 2,583,604 _RS31310 BPHYT_RS31315: BPHYT_RS31315 - cytochrome C, at 2,583,850 to 2,584,185 _RS31315 Position (kb) 2582 2583 2584Strain fitness (log2 ratio) -2 -1 0 1at 2581.313 kb on + strandat 2581.519 kb on - strand, within BPHYT_RS31300at 2581.643 kb on - strand, within BPHYT_RS31300at 2581.765 kb on - strand, within BPHYT_RS31300at 2582.677 kb on - strand, within BPHYT_RS31305at 2582.732 kb on + strand, within BPHYT_RS31305at 2583.221 kb on - strandat 2583.734 kb on - strandat 2584.195 kb on + strandat 2584.220 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 36hr PHYTO1 MOI 1
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2,581,313 + -0.2
2,581,519 - BPHYT_RS31300 0.18 -0.3
2,581,643 - BPHYT_RS31300 0.34 +1.1
2,581,765 - BPHYT_RS31300 0.49 -0.7
2,582,677 - BPHYT_RS31305 0.43 -2.3
2,582,732 + BPHYT_RS31305 0.49 -1.4
2,583,221 - -0.1
2,583,734 - +0.6
2,584,195 + -0.3
2,584,220 + -1.3

Or see this region's nucleotide sequence