Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS31195

Experiment: 36hr PHYTO1 MOI 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS31190 and BPHYT_RS31195 overlap by 4 nucleotidesBPHYT_RS31195 and BPHYT_RS31200 are separated by 8 nucleotidesBPHYT_RS31200 and BPHYT_RS31205 overlap by 4 nucleotides BPHYT_RS31190: BPHYT_RS31190 - ABC transporter permease, at 2,555,341 to 2,556,312 _RS31190 BPHYT_RS31195: BPHYT_RS31195 - peptide ABC transporter permease, at 2,556,309 to 2,557,220 _RS31195 BPHYT_RS31200: BPHYT_RS31200 - peptide ABC transporter substrate-binding protein, at 2,557,229 to 2,558,206 _RS31200 BPHYT_RS31205: BPHYT_RS31205 - peptide ABC transporter ATP-binding protein, at 2,558,203 to 2,559,246 _RS31205 Position (kb) 2556 2557 2558Strain fitness (log2 ratio) -2 -1 0 1at 2555.408 kb on + strandat 2555.530 kb on + strand, within BPHYT_RS31190at 2555.531 kb on - strand, within BPHYT_RS31190at 2555.531 kb on - strand, within BPHYT_RS31190at 2555.670 kb on - strand, within BPHYT_RS31190at 2555.670 kb on - strand, within BPHYT_RS31190at 2555.996 kb on - strand, within BPHYT_RS31190at 2556.271 kb on + strandat 2556.317 kb on + strandat 2556.600 kb on + strand, within BPHYT_RS31195at 2556.838 kb on - strand, within BPHYT_RS31195at 2556.888 kb on + strand, within BPHYT_RS31195at 2556.889 kb on - strand, within BPHYT_RS31195at 2557.378 kb on + strand, within BPHYT_RS31200at 2557.847 kb on - strand, within BPHYT_RS31200at 2557.916 kb on + strand, within BPHYT_RS31200at 2557.980 kb on - strand, within BPHYT_RS31200at 2558.108 kb on + strand, within BPHYT_RS31200

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Per-strain Table

Position Strand Gene LocusTag Fraction 36hr PHYTO1 MOI 1
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2,555,408 + +0.9
2,555,530 + BPHYT_RS31190 0.19 -1.8
2,555,531 - BPHYT_RS31190 0.20 -0.3
2,555,531 - BPHYT_RS31190 0.20 -1.2
2,555,670 - BPHYT_RS31190 0.34 -0.8
2,555,670 - BPHYT_RS31190 0.34 +1.0
2,555,996 - BPHYT_RS31190 0.67 +0.3
2,556,271 + -0.6
2,556,317 + -1.3
2,556,600 + BPHYT_RS31195 0.32 -1.4
2,556,838 - BPHYT_RS31195 0.58 -0.7
2,556,888 + BPHYT_RS31195 0.63 -2.2
2,556,889 - BPHYT_RS31195 0.64 -1.6
2,557,378 + BPHYT_RS31200 0.15 +0.2
2,557,847 - BPHYT_RS31200 0.63 -0.3
2,557,916 + BPHYT_RS31200 0.70 -0.8
2,557,980 - BPHYT_RS31200 0.77 +0.6
2,558,108 + BPHYT_RS31200 0.90 -0.9

Or see this region's nucleotide sequence