Experiment: 36hr PHYTO1 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS31185 and BPHYT_RS31190 are separated by 141 nucleotides BPHYT_RS31190 and BPHYT_RS31195 overlap by 4 nucleotides BPHYT_RS31195 and BPHYT_RS31200 are separated by 8 nucleotides
BPHYT_RS31185: BPHYT_RS31185 - LysR family transcriptional regulator, at 2,554,288 to 2,555,199
_RS31185
BPHYT_RS31190: BPHYT_RS31190 - ABC transporter permease, at 2,555,341 to 2,556,312
_RS31190
BPHYT_RS31195: BPHYT_RS31195 - peptide ABC transporter permease, at 2,556,309 to 2,557,220
_RS31195
BPHYT_RS31200: BPHYT_RS31200 - peptide ABC transporter substrate-binding protein, at 2,557,229 to 2,558,206
_RS31200
Position (kb)
2555
2556
2557 Strain fitness (log2 ratio)
-2
-1
0
1 at 2554.390 kb on + strand, within BPHYT_RS31185 at 2554.390 kb on + strand, within BPHYT_RS31185 at 2554.687 kb on + strand, within BPHYT_RS31185 at 2554.809 kb on - strand, within BPHYT_RS31185 at 2554.809 kb on - strand, within BPHYT_RS31185 at 2554.809 kb on - strand, within BPHYT_RS31185 at 2555.220 kb on - strand at 2555.220 kb on - strand at 2555.220 kb on - strand at 2555.220 kb on - strand at 2555.408 kb on + strand at 2555.530 kb on + strand, within BPHYT_RS31190 at 2555.531 kb on - strand, within BPHYT_RS31190 at 2555.531 kb on - strand, within BPHYT_RS31190 at 2555.670 kb on - strand, within BPHYT_RS31190 at 2555.670 kb on - strand, within BPHYT_RS31190 at 2555.996 kb on - strand, within BPHYT_RS31190 at 2556.271 kb on + strand at 2556.317 kb on + strand at 2556.600 kb on + strand, within BPHYT_RS31195 at 2556.838 kb on - strand, within BPHYT_RS31195 at 2556.888 kb on + strand, within BPHYT_RS31195 at 2556.889 kb on - strand, within BPHYT_RS31195
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO1 MOI 1 remove 2,554,390 + BPHYT_RS31185 0.11 +0.9 2,554,390 + BPHYT_RS31185 0.11 -0.8 2,554,687 + BPHYT_RS31185 0.44 -0.2 2,554,809 - BPHYT_RS31185 0.57 -0.5 2,554,809 - BPHYT_RS31185 0.57 -0.5 2,554,809 - BPHYT_RS31185 0.57 -1.2 2,555,220 - -1.2 2,555,220 - -0.2 2,555,220 - +0.1 2,555,220 - +0.8 2,555,408 + +0.9 2,555,530 + BPHYT_RS31190 0.19 -1.8 2,555,531 - BPHYT_RS31190 0.20 -0.3 2,555,531 - BPHYT_RS31190 0.20 -1.2 2,555,670 - BPHYT_RS31190 0.34 -0.8 2,555,670 - BPHYT_RS31190 0.34 +1.0 2,555,996 - BPHYT_RS31190 0.67 +0.3 2,556,271 + -0.6 2,556,317 + -1.3 2,556,600 + BPHYT_RS31195 0.32 -1.4 2,556,838 - BPHYT_RS31195 0.58 -0.7 2,556,888 + BPHYT_RS31195 0.63 -2.2 2,556,889 - BPHYT_RS31195 0.64 -1.6
Or see this region's nucleotide sequence