Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS29225

Experiment: 36hr PHYTO1 MOI 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS29220 and BPHYT_RS29225 are separated by 186 nucleotidesBPHYT_RS29225 and BPHYT_RS29230 are separated by 126 nucleotidesBPHYT_RS29230 and BPHYT_RS29235 are separated by 24 nucleotides BPHYT_RS29220: BPHYT_RS29220 - hypothetical protein, at 2,111,904 to 2,112,644 _RS29220 BPHYT_RS29225: BPHYT_RS29225 - diguanylate phosphodiesterase, at 2,112,831 to 2,114,900 _RS29225 BPHYT_RS29230: BPHYT_RS29230 - hypothetical protein, at 2,115,027 to 2,115,833 _RS29230 BPHYT_RS29235: BPHYT_RS29235 - cell shape determination protein CcmA, at 2,115,858 to 2,116,292 _RS29235 Position (kb) 2112 2113 2114 2115Strain fitness (log2 ratio) -1 0 1 2at 2112.160 kb on + strand, within BPHYT_RS29220at 2112.207 kb on - strand, within BPHYT_RS29220at 2112.208 kb on + strand, within BPHYT_RS29220at 2112.252 kb on - strand, within BPHYT_RS29220at 2112.458 kb on - strand, within BPHYT_RS29220at 2112.560 kb on - strand, within BPHYT_RS29220at 2112.578 kb on - strandat 2112.772 kb on - strandat 2112.829 kb on - strandat 2112.894 kb on + strandat 2113.101 kb on + strand, within BPHYT_RS29225at 2113.114 kb on - strand, within BPHYT_RS29225at 2113.349 kb on + strand, within BPHYT_RS29225at 2113.401 kb on + strand, within BPHYT_RS29225at 2114.050 kb on - strand, within BPHYT_RS29225at 2114.050 kb on - strand, within BPHYT_RS29225at 2114.999 kb on - strandat 2115.040 kb on + strandat 2115.436 kb on - strand, within BPHYT_RS29230at 2115.442 kb on + strand, within BPHYT_RS29230at 2115.442 kb on + strand, within BPHYT_RS29230

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Per-strain Table

Position Strand Gene LocusTag Fraction 36hr PHYTO1 MOI 1
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2,112,160 + BPHYT_RS29220 0.35 +0.3
2,112,207 - BPHYT_RS29220 0.41 -0.5
2,112,208 + BPHYT_RS29220 0.41 -0.8
2,112,252 - BPHYT_RS29220 0.47 -1.1
2,112,458 - BPHYT_RS29220 0.75 +0.1
2,112,560 - BPHYT_RS29220 0.89 -0.2
2,112,578 - -0.1
2,112,772 - +0.3
2,112,829 - +0.1
2,112,894 + -0.2
2,113,101 + BPHYT_RS29225 0.13 -0.2
2,113,114 - BPHYT_RS29225 0.14 -1.3
2,113,349 + BPHYT_RS29225 0.25 +0.1
2,113,401 + BPHYT_RS29225 0.28 +2.6
2,114,050 - BPHYT_RS29225 0.59 -0.1
2,114,050 - BPHYT_RS29225 0.59 -0.8
2,114,999 - -0.8
2,115,040 + +0.1
2,115,436 - BPHYT_RS29230 0.51 -0.2
2,115,442 + BPHYT_RS29230 0.51 +0.1
2,115,442 + BPHYT_RS29230 0.51 +0.3

Or see this region's nucleotide sequence